Salinity shock in jatropha curcas leaves is more pronounced during recovery than during stress time

Abstract

To verify the possible morphological and ultrastructural differences in the Jatropha curcas leaves, in response to high-intensity salt stress, three genotypes were evaluated (CNPAE183, JCAL171, and CNPAE218). In all the genotypes, 750mM NaCl, added to the nutrient solution, was applied to test its salt tolerance. For the analysis, the leaves were collected at three time points: (i) before stress (time 0 hour); (ii) during stress time (time 50 hours); and (iii) in the recovery period (time 914 hours) when the stressed plants recovered and demonstrated measurements of net photosynthetic with values similar to those demonstrated by the control plants. We showed that regardless of the genotype, saline shock caused an increase in the thickness of the mesophyll, and after the removal of NaCl, the thicker mesophyll remained in the JCAL171 and CNPAE218 genotypes, while the values observed in the CNPAE183 genotype were similar to those before stress. Scanning electron microscopy indicated that the stomata of CNPAE183 are smaller and have a stomatal index higher than the values demonstrated in JCAL171 and CNPAE218. Therefore, among the genotypes analyzed, CNPAE183 demonstrates that it could be considered a promising genotype for future studies of genetic improvement that seek elite genotypes tolerant to salinity.

Comparative analysis of salt-induced changes in the leaves proteome of two contrasting Jatropha curcas genotypes

“…To effect of comparison, 750 mM L -1 of NaCl, corresponding to 46.8 dS m -1 of electrical conductivity, obtained after linear regression between [NaCl] and its respective electrical conductivity. Silva-Santos, Corte-Real [11] corroborating this findings, describes the leaf anatomy of both J. curcas genotype studied here. These authors describes that CNPAE183 morphoanatomically and physiologically more plastic than CNPAE218.…”

“…The all experiments was performed under randomized block design containing two genotypes (CNPAE183, and CNPAE218), one saline concentrations (750 mM L -1 of NaCl) added to Hoagland nutrient solution and 4 replicates. The saline concentration used in this study merged after two previously study of our research group [10,11]. Corte-Real, Miranda [10] describes that while CNPAE183 readily recovered our net photosynthesis after salt stress alleviation, CNPAE218 never recovered our net photosynthesis, since even after 914 hours of salt alleviation its net photosynthesis were negative or almost zero.…”

“…Originally, this study was designed to compare six different genotypes of Jatropha curcas. However, based in the previous study of our research group [10,11],…”

“…The performance of J. curcas under salt stress has been the target of several physiological studies [9][10][11][12][13][14]. Recently, some studies [10][11][12] have been described J. curcas genotypes more tolerant do salinity than others.…”

“…The performance of J. curcas under salt stress has been the target of several physiological studies [9][10][11][12][13][14]. Recently, some studies [10][11][12] have been described J. curcas genotypes more tolerant do salinity than others. Although, J. curcas is a relatively drought-tolerant plant [15][16][17], it is also considered a salt-sensitive one [12,18,19], while others classify it as moderately tolerant to salt stress [13].…”