Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds

Costa, Rafael Oliveira de Araújo; Matias, Lídia Leonize Rodrigues; Passos, Thaís Souza; Queiroz, Jaluza Luana Carvalho de; Carvalho, Fabiana Maria Coimbra de; Maciel, Bruna Leal Lima; Uchôa, Adriana Ferreira; Amado, Isabel Rodríguez; Gonçalves, Catarina; Pastrana, Lorenzo; Morais, Ana Heloneida de Araújo

doi:10.1016/j.fufo.2020.100001

Cited by 11 publications

(26 citation statements)

References 45 publications

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“…The obtained micrographs ( Figure 1 C,D) showed that both nanoencapsulated presented intact particles, spherical shape, smooth surface, and nanometric physical size. These results are in agreement with those found by Queiroz et al [ 28 ], Matias et al [ 30 ], and Costa et al [ 29 ].…”

Section: Resultssupporting

confidence: 94%

“…These results prove the obtaining of nanometric particles with homogeneous size distribution [ 31 ]. For ECW, these results are close to those obtained by Matias et al (117.4 nm; 0.37) [ 30 ] and Costa et al (118 nm; 0.373) [ 29 ]. For the CW, it was observed that the results obtained were moderately superior when compared to those obtained by Matias et al (123.9 nm; 0.23) [ 30 ].…”

Section: Resultssupporting

confidence: 90%

“…The 12.5% SDS-PAGE gel stained with silver nitrate ( Figure 1 B) confirms the isolation of TTI with the presence of protein bands with a predominance of molecular mass around 20 kDa. The isolation and characterization of trypsin inhibitors from tamarind seeds have been well reported in the literature [ 23 , 24 , 25 , 26 , 27 , 29 , 30 ]. Therefore, the biochemical characteristics obtained in this study corroborate with previous studies.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, new studies have been developed on the bioactive effects of a nanoformulation based on TTI isolate, chitosan, and whey protein isolate (ECW), which has a spherical shape, smooth surface, size close to 100 nm, and potentiation of antitrypsin activity of the TTI [ 28 ]. In addition, ECW presented safe in vitro and in vivo toxicological evaluation [ 29 ] and induced a significant reduction in glutamic oxalacetic transaminase (AST) in an experiment with Wistar rats fed the HGLI diet mentioned above. In a third study with the same experimental model, ECW demonstrated a hypoglycemic effect in vivo [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study

Aguiar

Queiroz

Santos

et al. 2021

IJMS

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Several studies have sought new therapies for obesity and liver diseases. This study investigated the effect of the trypsin inhibitor isolated from tamarind seeds (TTI), nanoencapsulated in chitosan and whey protein isolate (ECW), on the liver health status of the Wistar rats fed with a high glycemic index (HGLI) diet. The nanoformulations without TTI (CW) and ECW were obtained by nanoprecipitation technique, physically and chemically characterized, and then administered to the animals. The adult male Wistar rats (n = 20) were allocated to four groups: HGLI diet + water; standard diet + water; HGLI diet + ECW (12.5 mg/kg); and HGLI diet + CW (10.0 mg/kg), 1 mL per gagave, for ten days. They were evaluated using biochemical and hematological parameters, Fibrosis-4 Index for Liver Fibrosis (FIB-4), AST to Platelet Ratio Index (APRI) scores, and liver morphology. Both nanoparticles presented spherical shape, smooth surface, and nanometric size [120.7 nm (ECW) and 136.4 nm (CW)]. In animals, ECW reduced (p < 0.05) blood glucose (17%), glutamic oxalacetic transaminase (39%), and alkaline phosphatase (24%). Besides, ECW reduced (p < 0.05) APRI and FIB-4 scores and presented a better aspect of hepatic morphology. ECW promoted benefits over a liver injury caused by the HGLI diet.

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Section: Resultssupporting

confidence: 94%

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study

Aguiar

Queiroz

Santos

et al. 2021

IJMS

Self Cite

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“…To assess the safety of administration by gavage in addition to maintaining the inhibitory activity, the encapsulated TTI (ECW) was evaluated in a preclinical obesity study. Costa ( 67 ) also assessed cytotoxicity using the Caco-2 and CCD-18Co strains (human intestinal cells). The cytotoxicity assay exceeded 70% cell viability for Caco-2 and CCD-18Co when exposed to different concentrations of ECW.…”

Section: Resultsmentioning

confidence: 99%

Tamarind (Tamarindus indica L.) Seed a Candidate Protein Source with Potential for Combating SARS-CoV-2 Infection in Obesity

Morais

Medeiros

et al. 2021

dti

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Introduction: Obesity and coronavirus disease (COVID)-19 are overlapping pandemics, and one might worsen the other. Methods: This narrative review discusses one of the primary mechanisms to initiate acute respiratory distress syndrome, uncontrolled systemic inflammation in COVID-19, and presents a potential candidate for adjuvant treatment. Blocking the S protein binding to angiotensin-converting enzyme 2 (ACE-2) and the 3C-like protease (3CL pro) is an effective strategy against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Results: Host proteases such as FURIN, trypsin, and transmembrane serine protease 2 (TMPRSS) act in S protein activation. Tamarind trypsin inhibitor (TTI) shows several beneficial effects on the reduction of inflammatory markers (tumor necrosis factor α [TNF-α], leptin) and biochemical parameters (fasting glycemia, triglycerides, and very low-density lipoprotein [VLDL]), in addition to improving pancreatic function and mucosal integrity in an obesity model. TTI may inhibit the action of proteases that collaborate with SARS-CoV-2 infection and the neutrophil activity characteristic of lung injury promoted by the virus. Conclusion: Thus, TTI may contribute to combating two severe overlapping problems with high cost and social complex implications, obesity and COVID-19.

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Interaction between insulin receptor and a peptide derived from a trypsin inhibitor purified from tamarind seed: An in silico screening of insulin-like peptides

Francisca T. Gomes,

de Medeiros,

Bezerra

et al. 2024

Arabian Journal of Chemistry

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Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds

Cited by 11 publications

References 45 publications

Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study

Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study

Tamarind (Tamarindus indica L.) Seed a Candidate Protein Source with Potential for Combating SARS-CoV-2 Infection in Obesity

Interaction between insulin receptor and a peptide derived from a trypsin inhibitor purified from tamarind seed: An in silico screening of insulin-like peptides

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