Safety and bioactive potential of nanoparticles containing Cantaloupe melon (Cucumis melo L.) carotenoids in an experimental model of chronic inflammation

Medeiros, Isaiane; Oliveira, Grazielle Louise Ribeiro de; Queiroz, Jaluza Luana Carvalho de; Gomes, Camila de Carvalho; Carvalho, Fabiana Maria Coimbra de; Lima, Maíra Conceição Jerônimo de Souza; Serquiz, Alexandre Coelho; Santos, Pedro Paulo de Andrade; Camillo, Christina da Silva; Maciel, Bruna Leal Lima; Morais, Ana Heloneida de Araújo; Passos, Thaís Souza

doi:10.1016/j.btre.2020.e00567

Cited by 11 publications

(22 citation statements)

References 33 publications

(36 reference statements)

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“…Nanoparticles containing the crude extract rich in carotenoids from the cantaloupe melon pulp in porcine gelatin (EPG) have already been produced by Medeiros et al and Oliveira et al In this study, EPG showed the same physicochemical characteristics and incorporation efficiency of carotenoids demonstrated by Medeiros et al, Medeiros et al, and Oliveira et al even with the obtainment of new batches, which effectively contributed to the investigations in the present study. These findings showed that the replicated method used in this study achieved the desired characteristics, overcoming the difficulties related to reproducibility in obtaining nanoparticles.…”

Section: Discussionsupporting

confidence: 69%

“…40,41 An effective way to improve these barriers has been to use nanotechnology to promote the encapsulation of these compounds, obtaining engineered nanoparticles based on delivery systems. 42 Nanoparticles containing the crude extract rich in carotenoids from the cantaloupe melon pulp in porcine gelatin (EPG) have already been produced by Medeiros et al 20 and Oliveira et al 21 In this study, EPG showed the same physicochemical characteristics and incorporation efficiency of carotenoids demonstrated by Medeiros et al, 19 Medeiros et al, 20 and Oliveira et al 21 even with the obtainment of new batches, which effectively contributed to the investigations in the present study. These findings showed that the replicated method used in this study 1 Age-appropriate retinol concentration based on Blomhoff et al 29 2 Retinol excess for age based on Blomhoff et al 29 Images of the livers were recorded after immersion in formalin (4%) to evaluate the superficial color.…”

Section: ■ Discussionsupporting

confidence: 65%

“…achieved the desired characteristics, overcoming the difficulties related to reproducibility in obtaining nanoparticles. Because of the low toxicity and bioactive potential of EPG promoted in the liver, 20 the present study investigated the effect of EPG on dietary intake, retinol, and weight in Wistar rats with diet-induced obesity. Besides, retinol concentrations in the liver and liver function were analyzed by determining liver scores to evaluate the changes caused by the ingestion of these nanoparticles containing CE under the liver of animals with obesity.…”

Section: ■ Discussionmentioning

confidence: 99%

“…For the characterization of EPG, scanning electron microscopy (SEM) analysis was performed to assess the morphology of the particles, laser diffraction to determine the diameter of nanoparticles, and Fourier transform infrared spectroscopy (FTIR) to evaluate the chemical interactions present in the system obtained according to Medeiros et al 20 In addition, the determination of the incorporation efficiency (IE) of carote- Preclinical Study. This study evaluated the effect of CE and EPG on the concentration of retinol in the liver of Wistar rats (male adults) with obesity induced by the high glycemic index and high glycemic load (HGLI) diet.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Therefore, this study continues the experiment conducted by Medeiros et al, 20 evaluating the effect of EPG on dietary intake, retinol intake, and body weight and the influence on the concentration of hepatic vitamin A and liver damage assessment scores in Wistar rats (male adults) with obesity induced by a high glycemic index and high glycemic load diet (HGLI diet).…”

Section: ■ Introductionmentioning

confidence: 99%

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Nanoparticles Loaded with a Carotenoid-Rich Extract from Cantaloupe Melon Improved Hepatic Retinol Levels in a Diet-Induced Obesity Preclinical Model

et al. 2023

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The study evaluated the effect of the carotenoid-rich extract from cantaloupe melon (CE) nanoencapsulated in porcine gelatin (EPG) on hepatic retinol concentration and liver damage scores in Wistar rats with obesity induced by high glycemic index and high glycemic load diet (HGLI diet). For 17 days, animals were fed the HGLI diet. They were divided into three groups and treated for 10 days [HGLI diet + water, HGLI diet + CE (12.5 mg/kg), and HGLI diet + EPG (50 mg/kg)]. The groups were evaluated for dietary intake, retinol, weight variation, hematological parameters, fasting glucose, lipid profile, hepatic retinol concentration, AST/ALT ratio, FIB-4 (Fibrosis-4 Index for Liver Fibrosis), and APRI (AST to Platelet Ratio Index) scores to evaluate the effects on the liver. Animals treated with EPG showed a lower dietary intake (p < 0.05). No significant weight change was detected in the evaluated groups (p > 0.05). The EPG-treated group had significantly higher concentrations (p < 0.05) of hepatic retinol [266 (45) μg/g] than the untreated group [186 (23.8) μg/g] and the one treated with CE [175 (8.08) μg/g]. Liver damage assessment scores did not show significant differences, but the lowest means were observed in the group treated with EPG. The nanoencapsulation of the extract rich in beta-carotene promoted reduced food consumption and increased hepatic retinol without causing significant changes in liver damage scores. Thus, EPG is a candidate for future clinical studies to evaluate the beneficial effects of treating diseases involving vitamin A deficiencies.

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