2018
DOI: 10.7150/thno.22552
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Abstract: Rationale: Mxi1 is regarded as a potential tumor suppressor protein that antagonizes the transcriptional activity of proto-oncogene Myc. However, the clinical significances and underlying mechanisms by which Mxi1 is regulated in lung cancer remain poorly understood.Methods: Mass spectrometry analysis and immunoprecipitation assay were utilized to detect the protein-protein interaction. The phosphorylation of Mxi1 was evaluated by in vitro kinase assays. Poly-ubiquitination of Mxi1 was examined by in vivo ubiqu… Show more

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Cited by 34 publications
(45 citation statements)
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“…The siR-NAs ON-TARGETplus SMARTpool for Mxi1 were purchased from Dharmacon. The siRNAs targeting UBE2O and β-Trcp were as follows: si-UBE2O#1: 5-GGUUGUAGAGUUGAAAGUUTT-3; si-UBE2O#2: 5-CCACCCAGUGUGAAACCAATT-3; si-β-Trcp: 5-AAGUGGAAUUUGUGGAACAUC-3 [20,21].…”
Section: Rna Interferencementioning
confidence: 99%
“…The siR-NAs ON-TARGETplus SMARTpool for Mxi1 were purchased from Dharmacon. The siRNAs targeting UBE2O and β-Trcp were as follows: si-UBE2O#1: 5-GGUUGUAGAGUUGAAAGUUTT-3; si-UBE2O#2: 5-CCACCCAGUGUGAAACCAATT-3; si-β-Trcp: 5-AAGUGGAAUUUGUGGAACAUC-3 [20,21].…”
Section: Rna Interferencementioning
confidence: 99%
“…Cells Synchronization: HeLa cells were synchronized at the G1/S boundary by performing a double thymidine block-and-release treatment as previously described, [54,70] and then were released into normal media at indicated time: 3 h (S-phase cells), 6 h (G2-phase cells), 9 h with incubation of 100 ng mL −1 nocodazole (M-phase cells) and 11 h (G1-phase cells). Cells were harvested and subjected to indicated experiments.…”
Section: Methodsmentioning
confidence: 99%
“…[26] Phospho-Histone H3 and Phospho-Histone H2AX Staining: The procedure was performed as described previously. [53,55,70] Briefly, cells were incubated with CPT (10 × 10 −9 m) for 1 h or left untreated and subsequently grown in the presence of paclitaxel (Taxol; 2 × 10 −6 m) for indicated times and then the cells were harvested and fixed in 70% ethanol at −20°C overnight. The cells were resuspended in 1 mL of 0.25% Triton X-100 in PBS, and rotated at 4°C for 15 min.…”
Section: Methodsmentioning
confidence: 99%
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“…SCF b-TrCP ubiquitin ligase is also responsible for ATF4 degradation relying on a phosphorylation-dependent mechanism and then activates the cAMP pathway [44]. Besides, SCF b-TrCP ubiquitin ligase regulates gene expression to manage an array of physiological processes by regulating amounts of transcription factors for degradation, including CREB-H [45], C-MYC [46], FOXO3 [47] p53 [48], p63c [49], MXI1 [50], NRF1-3 [51][52][53], and Yan/Tel [54].…”
Section: Substrates Of Scf B-trcp Ubiquitin Ligase In Gene Transcriptionmentioning
confidence: 99%