(S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds

Kienesberger, Bernhard; Obermüller, Beate; Singer, Georg; Mittl, Barbara; Grabherr, Reingard; Mayrhofer, Sigrid; Heinl, Stefan; Stadlbauer, Vanessa; Horvath, Angela; Miekisch, Wolfram; Fuchs, Patricia; Klymiuk, Ingeborg; Till, Holger; Castellani, Christoph

doi:10.3390/ijms22126424

Cited by 5 publications

(3 citation statements)

References 50 publications

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“…Nonetheless, this feedback mechanism is unclear. Muribaculum may also be linked to in ammatory processes [53,54]. These ve bacterial genera are thought to mediate the effect of JGP.…”

Section: Discussionmentioning

confidence: 99%

Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles

Li,

Cui,

Zheng

et al. 2024

Preprint

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Objective Immunological liver injury (ILI) is a common liver disease associated with the microbiota-gut-liver axis. Jian Gan powder (JGP) has protective and therapeutic effects on hepatitis virus-induced ILI in the clinic; nonetheless, underlying mechanisms are unclear.We aim to investigate the hepatoprotective effect of JGP in a mouse model of ILI. Methods The mouse model was established using Bacillus Calmette-Guérin (BCG) plus lipopolysaccharide (LPS). After JGP treatment (7.5, 15, or 30 g/kg), serum, liver, and fresh fecal samples were analyzed. 16S rRNA gene sequencing and untargeted metabolomics profiling were performed to assess the role of JGP on the gut microbiota and its metabolites. Results JGP treatment markedly reduced serum IFN-γ, IL-6, IL-22, and hepatic p-STAT3 (phosphorylated transducer and activator of transcription-3) expression. In contrast, JGP increased the percentage of proliferating cell nuclear antigen-positive liver cells in treated mice. Fecal 16S rRNA gene sequencing revealed that JGP treatment restored the levels of Alloprevotella, Burkholderia-Caballeronia-Paraburkholderia, Muribaculum, Streptococcus, and Stenotrophomonas. Additionally, metabolomics analysis of fecal samples showed that JGP restored the levels of allylestrenol, eplerenone, phosphatidylethanolamine (PE) (P-20:0/0:0), sphingomyelin (SM) d27:1, soyasapogenol C, chrysin, and soyasaponin I. Conclusions JGP intervention improves ILI by restoring gut microbiota and modifying its metabolic profiles. These results provide a novel insight into the mechanism of JGP in treating ILI and the scientific basis to support its clinical application.

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“…Nonetheless, this feedback mechanism is unclear. Muribaculum may also be linked to in ammatory processes [53,54]. These ve bacterial genera are thought to mediate the effect of JGP.…”

Section: Discussionmentioning

confidence: 99%

Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles

Li,

Cui,

Zheng

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Nonetheless, this feedback mechanism is unclear. Muribaculum may also be linked to inflammatory processes [56,57]. These five bacterial genera are thought to mediate the effect of JGP.…”

Section: Discussionmentioning

confidence: 99%

Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles

Li,

Cui,

Zheng

et al. 2024

Eur J Med Res

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Background Immunological liver injury (ILI) is a common liver disease associated with the microbiota-gut-liver axis. Jian Gan powder (JGP) exhibits both protective and therapeutic effects on hepatitis virus-induced ILI in the clinic. However, the underlying mechanisms remain elusive. The aim of this study is to investigate the hepatoprotective effects and associated mechanisms of JGP in the context of gut microbiota, utilizing a mouse model of ILI. Methods The mouse model was established employing Bacillus Calmette-Guérin (BCG) plus lipopolysaccharide (LPS). Following treatment with JGP (7.5, 15, or 30 g/kg), serum, liver, and fresh fecal samples were analyzed. 16S rRNA gene sequencing and untargeted metabolomics profiling were performed to assess the role of JGP on the gut microbiota and its metabolites. Results JGP treatment markedly reduced serum IFN-γ, IL-6, IL-22, and hepatic p-STAT3 (phosphorylated transducer and activator of transcription-3) expression. In contrast, JGP increased the percentage of proliferating cell nuclear antigen-positive liver cells in treated mice. Fecal 16S rRNA gene sequencing revealed that JGP treatment restored the levels of Alloprevotella, Burkholderia-Caballeronia-Paraburkholderia, Muribaculum, Streptococcus, and Stenotrophomonas. Additionally, metabolomics analysis of fecal samples showed that JGP restored the levels of allylestrenol, eplerenone, phosphatidylethanolamine (PE) (P-20:0/0:0), sphingomyelin (SM) d27:1, soyasapogenol C, chrysin, and soyasaponin I. Conclusions JGP intervention improves ILI by restoring gut microbiota and modifying its metabolic profiles. These results provide a novel insight into the mechanism of JGP in treating ILI and the scientific basis to support its clinical application.

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“…All fecal samples were analyzed within 48 h. VOC samples were drawn from the headspace of the fecal samples, as previously reported [ 32 , 33 , 34 ]. VOCs were pre-concentrated with a commercially available solid phase micro extraction (SPME) fiber (carboxen/polymethylsiloxane, Supelco, Bellefonte, PA, USA), which was thermally desorbed and analyzed by gas chromatography/mass spectrometry (GC/MS).…”

Section: Methodsmentioning

confidence: 99%

Probiotic OMNi-BiOTiC® 10 AAD Reduces Cyclophosphamide-Induced Inflammation and Adipose Tissue Wasting in Mice

Obermüller,

Singer,

Kienesberger

et al. 2023

Nutrients

Self Cite

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Cancer therapy is often associated with severe side effects such as drug induced weight loss, also known as chemotherapy-induced cachexia. The aim of this study was to investigate the effects of a multispecies probiotic (OMNi-BiOTiC® 10 AAD) in a chemotherapy mouse model. A total of 24 male BALB/c mice were gavage-fed with the probiotic formulation or water, once a day for 3 weeks. In the third week, the mice received intraperitoneal cyclophosphamide. At euthanasia, the organs were dissected, and serum was sampled for cytokine analysis. Tight junction components, myosin light chain kinase, mucins, and apoptosis markers were detected in the ileum and colon using histological analyses and qRT-PCR. Lipolysis was analyzed by enzymatic activity assay, Western blotting analyses, and qRT-PCR in WAT. The fecal microbiome was measured with 16S-rRNA gene sequencing from stool samples, and fecal volatile organic compounds analysis was performed using gas chromatography/mass spectrometry. The probiotic-fed mice exhibited significantly less body weight loss and adipose tissue wasting associated with a reduced CGI58 mediated lipolysis. They showed significantly fewer pro-inflammatory cytokines and lower gut permeability compared to animals fed without the probiotic. The colons of the probiotic-fed animals showed lower inflammation scores and less goblet cell loss. qRT-PCR revealed no differences in regards to tight junction components, mucins, or apoptosis markers. No differences in microbiome alpha diversity, but differences in beta diversity, were observed between the treatment groups. Taxonomic analysis showed that the probiotic group had a lower relative abundance of Odoribacter and Ruminococcus-UCG014 and a higher abundance of Desulfovibrio. VOC analysis yielded no significant differences. The results of this study indicate that oral administration of the multispecies probiotic OMNi-BiOTiC® 10 AAD could mitigate cyclophosphamide-induced chemotherapy side effects.

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(S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds

Cited by 5 publications

References 50 publications

Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles

Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles

Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles

Probiotic OMNi-BiOTiC® 10 AAD Reduces Cyclophosphamide-Induced Inflammation and Adipose Tissue Wasting in Mice

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