S-nitrosated keratin composite mats with NO release capacity for wound healing

Wan, Xiuzhen; Liu, Shuai; Xin, Xuanxuan; Li, Pengfei; Dou, Jun; Han, Xiao; Kang, Inn‐Kyu; Yuan, Jiang; Chi, Bo; Shen, Jian

doi:10.1016/j.cej.2020.125964

Cited by 64 publications

(59 citation statements)

References 62 publications

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“…Hence, we next first evaluated whether Ce6@Arg‐ADP can further generate trace amounts of NO in vivo after exerting its highly efficient antibacterial effect to promote angiogenesis and epithelialization. As the half‐life of NO is extremely short (only 1–5 s) [ 64,65 ] and there are many practical challenges for detecting NO generation in vivo in real‐time, the NO generation behavior was evaluated via an in vitro simulation experiment.…”

Section: Resultsmentioning

confidence: 99%

L‐Arg‐Rich Amphiphilic Dendritic Peptide as a Versatile NO Donor for NO/Photodynamic Synergistic Treatment of Bacterial Infections and Promoting Wound Healing

Zhu

Tian

Yang

et al. 2021

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The development of alternative strategies for the efficient treatment of subcutaneous abscesses that do not require the massive use of antibiotics and surgical intervention is urgently needed. Herein, a novel synergistic antibacterial strategy based on photodynamic (PDT) and NO gas therapy is reported, in which, a PDT‐driven NO controllable generation system (Ce6@Arg‐ADP) is developed with l‐Arg‐rich amphiphilic dendritic peptide (Arg‐ADP) as a carrier. This carrier not only displays superior bacterial association and biofilm penetration performance, but also acts as a versatile NO donor. Following efficient penetration into the interior of the biofilms, Ce6@Arg‐ADP can rapidly produce massive NO via utilizing the H2O2 generated during PDT to oxidize Arg‐ADP to NO and l‐citrulline, without affecting singlet oxygen (1O2) production. The combination of 1O2 and the reactive by‐products of NO offers notable synergistic antibacterial and biofilm eradication effects. Importantly, following efficient elimination of all bacteria from the abscess site, Arg‐ADP can further generate trace quantities of NO to facilitate the angiogenesis and epithelialization of the wound tissues, thereby notably promotes wound healing. Together, this study clearly suggests that Arg‐ADP is a versatile NO donor, and the combination of PDT and NO represents a promising strategy for the efficient treatment of subcutaneous abscesses.

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Section: Resultsmentioning

confidence: 99%

L‐Arg‐Rich Amphiphilic Dendritic Peptide as a Versatile NO Donor for NO/Photodynamic Synergistic Treatment of Bacterial Infections and Promoting Wound Healing

Zhu

Tian

Yang

et al. 2021

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“…Additionally, keratins contain cell adhesion motifs similar in structure to extracellular matrix proteins (such as collagen or fibronectin), arginine-glycine-aspartate (RGD) and leucine-aspartate-valine (LDV), which can support cell attachment and proliferation [ 5 ]. These unique structures and biological properties make keratin the focus of the biomedical field, including wound dressing, tissue engineering and drug delivery [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. Nevertheless, the shortcomings of brittleness, poor mechanical properties and processing properties limit the practical use of keratin [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, the shortcomings of brittleness, poor mechanical properties and processing properties limit the practical use of keratin [ 13 , 14 ]. Synthetic or natural polymers are usually added as plasticizers and crosslinkers to form composite materials to improve these defects of keratin, such as poly(vinyl alcohol) (PVA), polylactic acid, chitosan, gelatin, poly(ethylene oxide) (PEO) and polyurethane [ 8 , 11 , 15 , 16 , 17 , 18 , 19 ]. Electrospun nanofibers prepared by blending keratin with PEO and PVA can further promote tissue regeneration, because they simulate the structure of a natural extracellular matrix (ECM) [ 17 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation

et al. 2021

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The main core of wound treatment is cell growth and anti-infection. To accelerate the proliferation of fibroblasts in the wound and prevent wound infections, various strategies have been tried. It remains a challenge to obtain good cell proliferation and antibacterial effects. Here, human hair kerateine (HHK)/poly(ethylene oxide) (PEO)/poly(vinyl alcohol) (PVA) nanofibers were prepared using cysteine-rich HHK, and then, silver nanoparticles (AgNPs) were in situ anchored in the sulfur-containing amino acid residues of HHK. After the ultrasonic degradation test, HHK/PEO/PVA nanofibrous mats treated with 0.005-M silver nitrate were selected due to their relatively complete structures. It was observed by TEM-EDS that the sulfur-containing amino acids in HHK were the main anchor points of AgNPs. The results of FTIR, XRD and the thermal analysis suggested that the hydrogen bonds between PEO and PVA were broken by HHK and, further, by AgNPs. AgNPs could act as a catalyst to promote the thermal degradation reaction of PVA, PEO and HHK, which was beneficial for silver recycling and medical waste treatment. The antibacterial properties of AgNP-HHK/PEO/PVA nanofibers were examined by the disk diffusion method, and it was observed that they had potential antibacterial capability against Gram-positive bacteria, Gram-negative bacteria and fungi. In addition, HHK in the nanofibrous mats significantly improved the cell proliferation of NIH3T3 cells. These results illustrated that the AgNP-HHK/PEO/PVA nanofibrous mats exhibited excellent antibacterial activity and the ability to promote the proliferation of fibroblasts, reaching our target applications.

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“…This technology was tested on ischemic chronic wounds and demonstrated enhanced healing by pro-angiogenesis, immunomodulation, and enhanced collagen synthesis. Finally, PU/gelatin/keratin electrospun hydrogels were produced in 2020 by Wan et al [ 93 ]. In this development, further enhancement was achieved by N-nitrosation of keratin to form a coupled nitrosothiol NO-donor.…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

“…In the case of a novel carrier, its physicochemical characterization is performed first. For instance, electrospun functionalized dressings have been characterized by techniques including attenuated total reflection-Fourier transform infrared spectroscopy for the verification of functional coupling of the NO-donor to the polymer carrier; this is verified with scanning electron microscopy by visualizing the morphology of the fibers [ 35 , 93 ]. In the case of hydrogel matrices, further physicochemical characterization has been often carried-out to obtain parameters that describe the swelling capacity, degradation rate, rheological properties, and compression strength [ 94 ].…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

Synthetic, Natural, and Semisynthetic Polymer Carriers for Controlled Nitric Oxide Release in Dermal Applications: A Review

2021

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Nitric oxide (NO•) is a free radical gas, produced in the human body to regulate physiological processes, such as inflammatory and immune responses. It is required for skin health; therefore, a lack of NO• is known to cause or worsen skin conditions related to three biomedical applications— infection treatment, injury healing, and blood circulation. Therefore, research on its topical release has been increasing for the last two decades. The storage and delivery of nitric oxide in physiological conditions to compensate for its deficiency is achieved through pharmacological compounds called NO-donors. These are further incorporated into scaffolds to enhance therapeutic treatment. A wide range of polymeric scaffolds has been developed and tested for this purpose. Hence, this review aims to give a detailed overview of the natural, synthetic, and semisynthetic polymeric matrices that have been evaluated for antimicrobial, wound healing, and circulatory dermal applications. These matrices have already set a solid foundation in nitric oxide release and their future perspective is headed toward an enhanced controlled release by novel functionalized semisynthetic polymer carriers and co-delivery synergetic platforms. Finally, further clinical tests on patients with the targeted condition will hopefully enable the eventual commercialization of these systems.

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S-nitrosated keratin composite mats with NO release capacity for wound healing

Cited by 64 publications

References 62 publications

L‐Arg‐Rich Amphiphilic Dendritic Peptide as a Versatile NO Donor for NO/Photodynamic Synergistic Treatment of Bacterial Infections and Promoting Wound Healing

L‐Arg‐Rich Amphiphilic Dendritic Peptide as a Versatile NO Donor for NO/Photodynamic Synergistic Treatment of Bacterial Infections and Promoting Wound Healing

Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation

Synthetic, Natural, and Semisynthetic Polymer Carriers for Controlled Nitric Oxide Release in Dermal Applications: A Review

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