Rutin can replace the use of three other antioxidants in the culture medium, maintaining the viability of sheep isolated secondary follicles

Lins, T.L.B.G.; Cavalcante, A.Y.P.; Santos, J.M.S.; Menezes, Vanúzia Gonçalves; Barros, Vanessa Raquel Pinto de; Barberino, Ricássio de Sousa; Bezerra, M. É. S.; Macedo, T. J. S.; Matos, Maria Helena Tavares de

doi:10.1016/j.theriogenology.2016.11.019

Cited by 38 publications

(36 citation statements)

References 31 publications

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“…Antioxidant compounds have been used to minimize oxidative stress and to promote growth after in vitro culture of ovarian follicles (Lins et al, ; Silva et al, ), including medicinal plant extracts (Barberino et al, ; Gouveia et al, ). However, in this study, no significant results were found in the ROS formation.…”

Section: Discussionmentioning

confidence: 99%

“…Isolation, selection, culture and follicular evaluation were performed according to Lins et al (). The ovarian cortical slices (1–2 mm thick) were cut from the ovarian surface using a surgical blade under sterile conditions and subsequently placed in MEM‐HEPES.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, the oocytes were recovered and intracellular reduced glutathione (GSH), ROS levels and mitochondrial activity were measured as previously described (Lins et al, ). Briefly, 4‐chloromethyl‐6.8‐difluoro‐7‐hydroxycoumarin (CellTracker® Blue; CMF2HC; Invitrogen Corporation), 2′, 7′‐ dichlorodihydrofluorescein diacetate (H2DCFDA; Invitrogen Corporation) and MitoTracker Red (MitoTracker® Red, CMXRos, Molecular Probes, Melbourne, Australia) were used to detect intracellular GSH, ROS and mitochondrial activity levels as blue, green and red fluorescence, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels

Nascimento

Silva

Alves

et al. 2018

Reprod Domestic Animals

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Contents This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in the culture medium of isolated ovine preantral follicles. HERP was characterized by high‐performance liquid chromatography (HPLC) and determination of flavonoid content, and the nanoparticles by the mean particle diameter, polydispersity index (PI) and encapsulation efficiency (EE). The effect of HERP (10 and 20 ηg/ml—HERP10 and HERP20 groups) and nanoparticles (NP10 and NP20 groups) on isolated secondary follicles cultured in vitro for 12 days was observed by morphological evaluation, oxidative stress markers (reactive oxygen species—ROS and glutathione—GSH) and active mitochondria. HPLC showed formononetin as the major compound in the HERP (63.92 ± 0.21 μg/mL). The content of flavonoids ranged from 2.14% to 11.00%. The nanoparticles showed mean diameter of 287.5 ± 3.9 and 479 ± 18.1 ηm; PI of 0.117 ± 0.018 and 0.316 ± 0.039; and EE of 67.15% and 41%, respectively, for the NP10 and NP20 groups. After 12 days of culture, HERP20 and NP20 increased (p < 0.05) the percentage of normal follicles compared to NP10. HERP20 showed significantly higher percentages of antrum formation than control medium (MEM) and NP10 (p < 0.05). HERP20 also showed an increase (p < 0.05) in mitochondrial activity compared to the other treatments, except NP20 (p > 0.05), and increased GSH levels (p < 0.05) compared to MEM and HERP10. In conclusion, use of HERP (20 ηg/ml) on in vitro culture of isolated ovine preantral follicles can increase antrum formation, mitochondrial activity and GSH levels.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels

Nascimento

Silva

Alves

et al. 2018

Reprod Domestic Animals

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“…Recently, a study has shown that rutin decreases lipid peroxidation, ROS and DNA fragmentation in thawed deer spermatozoa after incubation at 37 ºC (MATA-CAMPUZANO et al, 2012). Other authors demonstrated that rutin preserves the viability and motility of human spermatozoa when used in the sperm selection by swim-up procedure (MORETTI et al, 2012), besides being able to replace the addition of transferrin, selenium and ascorbic acid during 12-days in vitro culture of ovine secondary follicles, maintaining follicular viability and increasing GSH levels (LINS et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Effect of ovarian tissue storage in Morus nigra extract on the morphology and DNA fragmentation of ovine preantral follicles

Cavalcante

Barberino

Gouveia

et al. 2017

SCA

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This study demonstrated the effect of Morus nigra leaf extract during ovine ovarian tissue transportation on the survival and apoptosis of preantral follicles in vitro. High Performance Liquid Chromatography (HPLC) was used to determine the fingerprint chromatogram of the crude ethanolic extract. Four pairs of ovaries from four sheep were collected. The ovarian cortex was fragmented and one fragment was fixed in 10% buffered formaldehyde and processed for histological and TUNEL analyses (fresh control). The other fragments were placed in Minimal Essential Medium (MEM -control medium) or M. nigra extract (0.025; 0.05 or 0.1 mg/mL) and stored (simulating transport) at 4ºC for 6, 12 or 24 h. Preserved fragments (6 h) were also destined to histological and TUNEL analyses. HPLC analysis confirmed the presence of antioxidant compounds (rutin, isoquercetin e kaempferitrin) in the extract. There was a decrease (P < 0.05) in the percentage of morphologically normal preantral follicles after preservation in all treatments compared to the fresh control. The percentage of normal preantral follicles after preservation in M. nigra at 0.05 mg/mL for 6 h was higher (P < 0.05) than in MEM or 0.025 mg/ mL M. nigra and similar (P > 0.05) to 0.1 mg/mL of the extract. Apoptosis increased (P < 0.05) after conservation for 6 h in all treatments compared to the fresh control. Moreover, TUNEL positive cells decreased (P < 0.05) after preservation in 0.05 or 0.1 mg/mL M. nigra compared to MEM or 0.025 mg/ mL M. nigra. In conclusion, 0.05 mg/mL M. nigra extract can be used as a preservation medium for ovine ovarian tissue at 4°C for up to 6 h. ResumoEste estudo demonstrou o efeito do extrato das folhas de Morus nigra durante o transporte na sobrevivência e apoptose de folículos pré-antrais in vitro. A CLAE (Cromatografia Líquida de Alta Eficiência) foi usada para determinar a impressão digital cromatográfica do extrato etanólico. Foram coletados 4 pares de ovários de 4 ovelhas. O córtex ovariano foi fragmentado e um dos fragmentos foi fixado em formaldeído tamponado a 10% e destinado às análises histológica e de TUNEL (controle fresco). Os demais fragmentos foram colocados em Meio Essencial Mínimo (MEM -meio controle) ou extrato de M. nigra (0,025, 0,05 ou 0,1 mg/mL) e conservados (simulando transporte) à 4 ºC por 6, 12 ou 24 h. Os fragmentos conservados (6 h) também foram destinados às análises histológica e de TUNEL. A análise de CLAE confirmou a presença de compostos antioxidantes (rutina, isoquercetina e canferitrina) no extrato. Houve uma redução (P < 0,05) na percentagem de folículos pré-antrais morfologicamente normais após conservação em todos os tratamentos em comparação com o controle fresco. A percentagem de folículos pré-antrais normais após conservação em M. nigra a 0,05 mg/mL por 6 h foi maior (P < 0,05) do que em MEM ou M. nigra a 0,025 mg/mL e similar (P > 0,05) ao extrato a 0,1 mg/mL. A apoptose aumentou (P < 0,05) após conservação durante 6 h em todos os tratamentos em comparação com o controle fresco. Além diss...

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“…The flavonoid rutin decreased lipid peroxidation, reactive oxygen species (ROS) production and DNA fragmentation in thawed deer spermatozoa after incubation at 37°C (Mata‐Campuzano et al., ). After 12 days of in vitro culture of sheep ovarian secondary follicles, rutin was effective in replacing three antioxidants added to the medium (transferrin, selenium and ascorbic acid), maintaining follicular viability (Lins et al., ). Furthermore, kaempferitrin exerts antioxidant activity with similar potential to quercetin (de Sousa et al., ).…”

Section: Discussionmentioning

confidence: 99%

Supplemented Morus nigra extract‐based medium associated with FSH enables the survival and growth of isolated ovine secondary ovarian follicles

Cavalcante

Lins

Santos

et al. 2017

Reprod Domestic Animals

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The effects of Morus nigra ethanolic extract, without or with addition of supplements associated or not with FSH, on in vitro culture of ovine secondary follicles were evaluated. In experiment 1, isolated secondary follicles were cultured for 12 days in α-MEM alone (control) or in different concentrations of M. nigra extract (MN 0.025; 0.05 or 0.1 mg/ml). In experiment 2, culture media were α-MEM supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine and ascorbic acid (α-MEM ) or this medium associated with FSH (α-MEM + FSH), or 0.1 mg/ml M. nigra without supplements (MN 0.1) or supplemented (MN 0.1 ) without or with FSH (MN 0.1 + FSH). In experiment 1, 0.1 mg/ml M. nigra showed the highest percentages (p < .05) of normal follicles and fully grown oocytes, besides a higher follicular diameter than α-MEM and other M. nigra extract concentrations. Moreover, MN 0.1 showed lower (p < .05) glutathione (GSH) levels and similar (p > .05) mitochondrial activity compared to α-MEM. In experiment 2, MN 0.1 + FSH showed similar results (p > .05) to α-MEM + FSH for all parameters evaluated, except for the daily growth rate, which was higher (p < .05) in MN 0.1 + FSH. The GSH levels were higher in MEM than all treatments. In addition, oocytes from follicles cultured in MN 0.1 + FSH showed ability to resume meiosis. In conclusion, M. nigra extract (0.1 mg/ml) added by supplements and FSH can be an efficient medium for ovine secondary follicle development.

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Rutin can replace the use of three other antioxidants in the culture medium, maintaining the viability of sheep isolated secondary follicles

Cited by 38 publications

References 31 publications

Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels

Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels

Effect of ovarian tissue storage in Morus nigra extract on the morphology and DNA fragmentation of ovine preantral follicles

Supplemented Morus nigra extract‐based medium associated with FSH enables the survival and growth of isolated ovine secondary ovarian follicles

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