Ruscogenin glycoside (Lm-3) isolated fromLiriope muscariinhibits lymphocyte adhesion to extracellular matrix

Abstract

We examined the effects of ruscogenin glycoside (Lm-3), isolated from Liriope muscari, on lymphocyte adhesion to extracellular matrix. Adhesion of Jurkat cells activated by anti-CD3 to type I collagen was inhibited by Lm-3 in a concentration- and time-dependent manner. Lm-3 also inhibited the cell attachment to fibronectin and laminin. However, the saponin did not influence anti-CD3-induced cell proliferation and Mn2+-induced adhesion. Protein kinase C activator, phorbol 12,13-dibutyrate, significantly enhanced, while its inhibitor, chlorpromazine, almost completely blocked, the adhesion of anti-CD3-activated Jurkat cells to collagen. Against phorbol 12,13-dibutyrate-activated Jurkat cells, Lm-3 treatment, either before or after activation, significantly inhibited the cell adhesion to collagen. Lm-3 also inhibited the adhesion activated by both anti-CD3 and phorbol 12,13-dibutyrate. Similar inhibition by Lm-3 of the phorbol 12,13-dibutyrate-induced adhesion to collagen was also observed in lymphocytes freshly isolated from mice with contact dermatitis. Furthermore, Lm-3 significantly decreased the leucocyte accumulation in an animal model of experimental pleurisy. These results suggest that the blockade of lymphocyte adhesion to extracellular matrix through interference with the protein kinase C pathway may be one of the mechanisms by which Lm-3 exerts anti-inflammatory activity.

Possible Mechanism of the Anti-inflammatory Activity of Ruscogenin: Role of Intercellular Adhesion Molecule-1 and Nuclear Factor-κB

“…In addition, ruscogenin is also a major steroidal sapogenin of Radix Ophiopogon japonicus, a Chinese herb that has been used to treat acute and chronic inflammatory and cardiovascular diseases for thousands of years (4). The related pharmacological studies have been confirmed activities of the ethanol extract from Ophiopogon japonicus or ruscogenin glycoside in various aspects such as antiarrhythmia (5), anti-ischaemia (6), anti-thrombosis (7,8) effects; improving liver injury (9); and inhibiting inflammation (10,11). Our previous studies also showed that ruscogenin significantly inhibited the adhesion of leukocytes to ECV304 cells activated by tumor necrosis factor-α (TNF-α), while it had no remarkable effect on leukocyte adherence to resting ECV304 cells and cell viability, and also inhibited leukocyte migration in vivo.…”

Pleurotus ostreatus and Ruscus aculeatus Extracts Cause Non-Apoptotic Jurkat Cell Death

“…Extracts from this plant have been used in traditional medicine (Guarrera, 2005). Studies have shown that R. aculeatus extracts are active against blood disorders such as venous insufficiencies, edema, orthostatic hypotension, and inflammation (Liu et al, 2002;Huang et al, 2008;Mimaki et al, 1998). Notably, two saponin compounds found in the alcoholic extract of R. aculeatus inhibit the growth of the human promyelocytic leukemia cell line HL-60 (Lindequist, Niedermeyer & Julich, 2005).…”

Pharmaceutical strategies to prevent ventilator-associated pneumonia

“…Mice were sensitized by painting 0.1 mL of 1% picryl chloride in ethanol on the shaved skin of their abdomens. Five days later, they were challenged by painting 30 ·L of 1% picryl chloride in olive oil on the right ear lobe (Liu et al 2002). Eighteen hours later, the thickness of the right ear was measured against the left with a digimatic micrometer (0.001 mm; Mitutoyo Co., Tokyo, Japan).…”