2011
DOI: 10.1002/jbmr.504
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: We have recently shown that a 150-bp Col10a1 distal promoter (−4296 to −4147 bp) is sufficient to direct hypertrophic chondrocyte-specific reporter (LacZ) expression in vivo. More recently, through detailed sequence analysis we identified two putative tandem-repeat Runx2 binding sites within the 3′-end of this 150-bp region (TGTGGG-TGTGGC, −4187 to −4176 bp). Candidate electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation, and transfection studies demonstrate that these putative Runx2 site… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

8
70
1

Year Published

2012
2012
2023
2023

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 66 publications
(79 citation statements)
references
References 45 publications
8
70
1
Order By: Relevance
“…Previous studies have suggested that the col10a1 promoter of vertebrates may contain several components that direct its tissue-specific gene expression, such as the proximal basal regulatory region, and negative and positive regulatory elements (Higashikawa et al, 2009;Li et al, 2011;Simoes et al, 2006;Zheng et al, 2003). Our study also supports the existence of different regulatory elements within the zebrafish col10a1 promoter at least for the osteoblast-specific program.…”
Section: Discussionsupporting
confidence: 85%
See 2 more Smart Citations
“…Previous studies have suggested that the col10a1 promoter of vertebrates may contain several components that direct its tissue-specific gene expression, such as the proximal basal regulatory region, and negative and positive regulatory elements (Higashikawa et al, 2009;Li et al, 2011;Simoes et al, 2006;Zheng et al, 2003). Our study also supports the existence of different regulatory elements within the zebrafish col10a1 promoter at least for the osteoblast-specific program.…”
Section: Discussionsupporting
confidence: 85%
“…Additionally, a negative element present in the -1.2-kb region may act when no positive signal is delivered, which could be a general mechanism for suppressing col10a1 expression in most cell types. Consistent with this possibility, the ~1.1-kb col10a1 promoter (similar to our ~1.3-kb construct) showed only a small amount of transactivation of the luciferase reporter in the Xenopus A6 kidney epithelial cell line (Higashikawa et al, 2009;Li et al, 2011;Simoes et al, 2006;Zheng et al, 2003). Therefore, tissue-specific col10a1 expression in vertebrates may depend on the activity of different elements within the promoter.…”
Section: Discussionsupporting
confidence: 84%
See 1 more Smart Citation
“…Binding around Col10a1 highlighted a previously identified hypertrophic DNA enhancer module 4 kb upstream of Col10a1 that is sufficient to direct hypertrophic chondrocyte-restricted reporter gene expression in vivo (Fig. 2F) (Chambers et al, 2002;Gebhard et al, 2004;Leung et al, 2011;Li et al, 2011;Riemer et al, 2002;Zheng et al, 2009).…”
Section: Co-occupancy Of the Chondrocyte Genome By Sox9 And Junmentioning
confidence: 75%
“…By contrast, Runx2 is essential for specifying the osteoblast lineage and directly regulates another transcription factor, osterix (Osx) (8,9), but is also expressed by HCs. It regulates Col10a1 and matrix metalloproteinase-13 (Mmp13) expression in HCs (10,11). Osx is expressed in prehypertrophic chondrocytes and osteoblasts and is essential for preosteoblast differentiation (9): it directly transactivates Col1a1, which encodes collagen I, a marker of differentiated osteoblasts.…”
mentioning
confidence: 99%