Route of Infection in Melioidosis

Barnes, Jodie L.; Ketheesan, Natkunam

doi:10.3201/eid1104.041051

Cited by 62 publications

(76 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our observations of neurotropism are not unique because bacteria have been cultured from brain in oral, intranasal, intravenous and intraperitoneal models of B. pseudomallei infection. 10 Neurologic infection in humans has been well documented, particularly in Australia. 1 Although intranasal mouse infection models probably cause direct infection of the brain by the olfactory nerve, 17 our results suggest that B. pseudomallei acquired enterally can cross the blood-brain barrier.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pathogenicity of High-Dose Enteral Inoculation of Burkholderia pseudomallei to Mice

West¹,

Myers²,

Limmathurotsakul³

et al. 2010

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Melioidosis is a frequently lethal tropical infection caused by the environmental saprophyte Burkholderia pseudomallei. Although transcutaneous inoculation and inhalation are considered the primary routes of infection, suggestive clinical evidence implicates ingestion as a possible alternative route. We show that in BALB/c and C57BL/6 mice, direct gastric inoculation of high doses of B. pseudomallei causes systemic infection that may be lethal or cause chronic disseminated infection. Mice may shed bacteria in the stool for weeks after infection, and high titers of B. pseudomallei-specific IgG are detectable. This report of enteric murine melioidosis supports further consideration of this route of infection.

show abstract

Section: Discussionmentioning

confidence: 99%

“…6 Experimental enteric infection has received relatively little consideration since the description of melioidosis in 1913 by Whitmore. [7][8][9][10] We developed a model of enteric melioidosis in mice to shed light on ingestion as a route of infection in humans.…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of High-Dose Enteral Inoculation of Burkholderia pseudomallei to Mice

West¹,

Myers²,

Limmathurotsakul³

et al. 2010

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…B. pseudomallei is being isolated increasingly in other parts of the world as well, likely due to greater awareness and surveillance for the organism (7,8). Infection with B. pseudomallei can be contracted via several routes, including subcutaneous (s.c.) inoculation, inhalation, and likely ingestion (9,10). The route of B. pseudomallei infection is correlated with the severity of infection, with inhalational infection generally associated with a more rapid disease course.…”

mentioning

confidence: 94%

Correlates of Immune Protection following Cutaneous Immunization with an Attenuated Burkholderia pseudomallei Vaccine

et al. 2013

View full text Add to dashboard Cite

Infections with the Gram-negative bacterium Burkholderia pseudomallei (melioidosis) are associated with high mortality, and there is currently no approved vaccine to prevent the development of melioidosis in humans. Infected patients also do not develop protective immunity to reinfection, and some individuals will develop chronic, subclinical infections with B. pseudomallei. At present, our understanding of what constitutes effective protective immunity against B. pseudomallei infection remains incomplete. Therefore, we conducted a study to elucidate immune correlates of vaccine-induced protective immunity against acute B. pseudomallei infection. BALB/c and C57BL/6 mice were immunized subcutaneously with a highly attenuated, Select Agent-excluded purM deletion mutant of B. pseudomallei (strain Bp82) and then subjected to intranasal challenge with virulent B. pseudomallei strain 1026b. Immunization with Bp82 generated significant protection from challenge with B. pseudomallei, and protection was associated with a significant reduction in bacterial burden in lungs, liver, and spleen of immunized mice. Humoral immunity was critically important for vaccine-induced protection, as mice lacking B cells were not protected by immunization and serum from Bp82-vaccinated mice could transfer partial protection to nonvaccinated animals. In contrast, vaccineinduced protective immunity was found to be independent of both CD4 and CD8 T cells. Tracking studies demonstrated uptake of the Bp82 vaccine strain predominately by neutrophils in vaccine-draining lymph nodes and by smaller numbers of dendritic cells (DC) and monocytes. We concluded that protection following cutaneous immunization with a live attenuated Burkholderia vaccine strain was dependent primarily on generation of effective humoral immune responses.

show abstract

“…DC migration has also been shown to facilitate the rapid dissemination of Bacillus anthracis spores from the lungs to the thoracic lymph nodes (using transgenic mice developed to specifically express green fluorescent protein [GFP] in DC only (27,28). Similarly, B. pseudomallei is an intracellular pathogen capable of persistence within different host cells and rapid systemic spread to multiple organs within hours of exposure (6,29,30). Recently, colonization of the brain in a murine model of neurological melioidosis was found to occur via the transmigration of B. pseudomallei-infected CD11b ϩ phagocytes across endothelial cells (31).…”

Section: Both Human and Murine DC Stimulated With Heat-killed B Pseumentioning

confidence: 99%

Migration of Dendritic Cells Facilitates Systemic Dissemination of Burkholderia pseudomallei

et al. 2014

Self Cite

View full text Add to dashboard Cite

Burkholderia pseudomallei, the etiological agent for melioidosis, is an important cause of community-acquired sepsis in northern Australia and northeast Thailand. Due to the rapid dissemination of disease in acute melioidosis, we hypothesized that dendritic cells (DC) could act as a vehicle for dissemination of B. pseudomallei. Therefore, this study investigated the effect of B. pseudomallei infection on DC migration capacity and whether migration of DC enabled transportation of B. pseudomallei from the site of infection. B. pseudomallei stimulated significantly increased migration of bone marrow-derived DC (BMDC), both in vitro and in vivo, compared to uninfected BMDC. Furthermore, migration of BMDC enabled significantly increased in vitro trafficking of B. pseudomallei and in vivo dissemination of B. pseudomallei to secondary lymphoid organs and lungs of C57BL/6 mice. DC within the footpad infection site of C57BL/6 mice also internalized B. pseudomallei and facilitated dissemination. Although DC have previously been shown to kill intracellular B. pseudomallei in vitro, the findings of this study demonstrate that B. pseudomallei-infected DC facilitate the systemic spread of this pathogen.

show abstract

Route of Infection in Melioidosis

Cited by 62 publications

References 9 publications

Pathogenicity of High-Dose Enteral Inoculation of Burkholderia pseudomallei to Mice

Pathogenicity of High-Dose Enteral Inoculation of Burkholderia pseudomallei to Mice

Correlates of Immune Protection following Cutaneous Immunization with an Attenuated Burkholderia pseudomallei Vaccine

Migration of Dendritic Cells Facilitates Systemic Dissemination of Burkholderia pseudomallei

Contact Info

Product

Resources

About