Roughness statistical influence on cell adhesion using profilometry and multiscale analysis

Giljean, Sylvain; Bigerelle, Maxence; Anselme, Karine

doi:10.1002/sca.21061

Cited by 28 publications

(22 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, similar effects of the nanoscale EP and the roughness of the CP coatings on SCs were studied using HLPSCs. As discussed in previous studies [ 22 , 23 , 24 ], an original statistical approach was developed for characterizing the relationship between surface roughness and cell adhesion. For two materials, 316L stainless steel and TiAl6V4 titanium alloy, a relationship between R a and cell adhesion has not been found.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Nanoscale Electrical Potential and Roughness of a Calcium Phosphate Surface Promotes the Osteogenic Phenotype of Stromal Cells

et al. 2018

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) and osteoblasts respond to the surface electrical charge and topography of biomaterials. This work focuses on the connection between the roughness of calcium phosphate (CP) surfaces and their electrical potential (EP) at the micro- and nanoscales and the possible role of these parameters in jointly affecting human MSC osteogenic differentiation and maturation in vitro. A microarc CP coating was deposited on titanium substrates and characterized at the micro- and nanoscale. Human adult adipose-derived MSCs (hAMSCs) or prenatal stromal cells from the human lung (HLPSCs) were cultured on the CP surface to estimate MSC behavior. The roughness, nonuniform charge polarity, and EP of CP microarc coatings on a titanium substrate were shown to affect the osteogenic differentiation and maturation of hAMSCs and HLPSCs in vitro. The surface EP induced by the negative charge increased with increasing surface roughness at the microscale. The surface relief at the nanoscale had an impact on the sign of the EP. Negative electrical charges were mainly located within the micro- and nanosockets of the coating surface, whereas positive charges were detected predominantly at the nanorelief peaks. HLPSCs located in the sockets of the CP surface expressed the osteoblastic markers osteocalcin and alkaline phosphatase. The CP multilevel topography induced charge polarity and an EP and overall promoted the osteoblast phenotype of HLPSCs. The negative sign of the EP and its magnitude at the micro- and nanosockets might be sensitive factors that can trigger osteoblastic differentiation and maturation of human stromal cells.

show abstract

Section: Discussionmentioning

confidence: 99%

“…No relation was found between the roughness amplitude, R a , and cell adhesion. The parameter that better discriminates adhesion is the mean distance between asperities ( S m ); however, this correlation is insufficiently strict, and several authors [ 22 , 23 , 24 ] have developed a new parameter called “adhesion power”.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale Electrical Potential and Roughness of a Calcium Phosphate Surface Promotes the Osteogenic Phenotype of Stromal Cells

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Grinding processes differ from 1000 to 4000 mesh papers and are only seldom followed by diamond paste polishing. Surface treatments such as mechanical polishing or electrolytic polishing enhance the corrosion resistance, while an increased surface roughness amplitude with a low surface organization increases the corrosion rate [38]. It has been previously demonstrated that surface roughness amplitude has an influence on the corrosion rate of biodegradable materials and consequently the concentration of released species [39,40].…”

Section: Amentioning

confidence: 99%

Are Fe-Based Stenting Materials Biocompatible? A Critical Review of In Vitro and In Vivo Studies

Scarcello

Lison

2019

JFB

View full text Add to dashboard Cite

Fe-based materials have increasingly been considered for the development of biodegradable cardiovascular stents. A wide range of in vitro and in vivo studies should be done to fully evaluate their biocompatibility. In this review, we summarized and analyzed the findings and the methodologies used to assess the biocompatibility of Fe materials. The majority of investigators drew conclusions about in vitro Fe toxicity based on indirect contact results. The setup applied in these tests seems to overlook the possible effects of Fe corrosion and does not allow for understanding of the complexity of released chemical forms and their possible impact on tissue. It is in particular important to ensure that test setups or interpretations of in vitro results do not hide some important mechanisms, leading to inappropriate subsequent in vivo experiments. On the other hand, the sample size of existing in vivo implantations is often limited, and effects such as local toxicity or endothelial function are not deeply scrutinized. The main advantages and limitations of in vitro design strategies applied in the development of Fe-based alloys and the correlation with in vivo studies are discussed. It is evident from this literature review that we are not yet ready to define an Fe-based material as safe or biocompatible.

show abstract

“…Several techniques involving etching and particle impact can be used to change topography and wettability [5]. A recent study involving isotropic abrasion of both 316L stainless steel and TiAl6V4 and introduction of human mesenchymal stem cells found no statistical effect of roughness on cell number or coverage at 48 h [6]. The main applications as a biomaterial are for femoral heads for total hip replacement where it has been used for more than a decade [7] and dental implants.…”

Section: Introductionmentioning

confidence: 98%

Osteoblast response to zirconia surfaces with different topographies

Herath

Silvio

Evans

2015

Materials Science and Engineering: C

View full text Add to dashboard Cite

Zirconia-3 mol% yttria ceramics were prepared with as-sintered, abraded, polished, and porous surfaces in order to explore the attachment, proliferation and differentiation of osteoblast-like cells. After modification, all surfaces were heated to 600°C to extinguish traces of organic contamination. All surfaces supported cell attachment, proliferation and differentiation but the surfaces with grain boundary grooves or abraded grooves provided conditions for enhanced initial cell attachment. Nevertheless, overall cell proliferation and total DNA were highest on the polished surface. Zirconia sintered at a lower temperature (1300°C vs. 1450°C) had open porosity and presented reduced proliferation as assessed by alamarBlue™ assay, possibly because the openness of the pores prevented cells developing a local microenvironment. All cells retained the typical polygonal morphology of osteoblast-like cells with variations attributable to the underlying surface notably alignment along the grooves of the abraded surface.

show abstract

Roughness statistical influence on cell adhesion using profilometry and multiscale analysis

Cited by 28 publications

References 30 publications

Nanoscale Electrical Potential and Roughness of a Calcium Phosphate Surface Promotes the Osteogenic Phenotype of Stromal Cells

Nanoscale Electrical Potential and Roughness of a Calcium Phosphate Surface Promotes the Osteogenic Phenotype of Stromal Cells

Are Fe-Based Stenting Materials Biocompatible? A Critical Review of In Vitro and In Vivo Studies

Osteoblast response to zirconia surfaces with different topographies

Contact Info

Product

Resources

About