Rottlerin Exhibits Antiangiogenic Effects <i>In vitro</i>

Valacchi, Giuseppe; Pecorelli, Alessandra; Sticozzi, Claudia; Torricelli, Claudia; Muscettola, Michela; Aldinucci, Carlo

doi:10.1111/j.1747-0285.2011.01121.x

Cited by 28 publications

(22 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies showed that rottlerin inhibited cancer cell growth and induced apoptosis in a variety of human cancers [13, 14, 41, 42]. Moreover, rottlerin could suppress growth of human pancreatic tumors in nude mice [43, 44]. We also found that overexpression of Cdc20 enhanced glioma cell proliferation, whereas depletion of Cdc20 suppressed cell growth in glioma cells.…”

Section: Discussionsupporting

confidence: 50%

“…Rottlerin was also exhibited its anti-angiogenesis function via inhibition of human microvascular endothelial cell proliferation and tube formation on Matrigel [31]. Moreover, rottlerin was reported to enhance IL-1β (interleukin-1β)-induced COX-2 (cyclooxygenase-2) expression via activation of p38 MAPK (mitogen-activated protein kinases) in breast cancer cells [32].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Rottlerin inhibits cell growth and invasion via down-regulation of Cdc20 in glioma cells

et al. 2016

View full text Add to dashboard Cite

Rottlerin, isolated from a medicinal plant Mallotus phillippinensis, has been demonstrated to inhibit cellular growth and induce cytoxicity in glioblastoma cell lines through inhibition of calmodulin-dependent protein kinase III. Emerging evidence suggests that rottlerin exerts its antitumor activity as a protein kinase C inhibitor. Although further studies revealed that rottlerin regulated multiple signaling pathways to suppress tumor cell growth, the exact molecular insight on rottlerin-mediated tumor inhibition is not fully elucidated. In the current study, we determine the function of rottlerin on glioma cell growth, apoptosis, cell cycle, migration and invasion. We found that rottlerin inhibited cell growth, migration, invasion, but induced apoptosis and cell cycle arrest. Mechanistically, the expression of Cdc20 oncoprotein was measured by the RT-PCR and Western blot analysis in glioma cells treated with rottlerin. We observed that rottlerin significantly inhibited the expression of Cdc20 in glioma cells, implying that Cdc20 could be a novel target of rottlerin. In line with this, over-expression of Cdc20 decreased rottlerin-induced cell growth inhibition and apoptosis, whereas down-regulation of Cdc20 by its shRNA promotes rottlerin-induced anti-tumor activity. Our findings indicted that rottlerin could exert its tumor suppressive function by inhibiting Cdc20 pathway which is constitutively active in glioma cells. Therefore, down-regulation of Cdc20 by rottlerin could be a promising therapeutic strategy for the treatment of glioma.

show abstract

Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Rottlerin inhibits cell growth and invasion via down-regulation of Cdc20 in glioma cells

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The transcription factor NFκB, a key component for the expression of many cellular genes, has been implicated in the signaling pathways of several growth factors produced by platelets, controlling cytokine expression and regulating inflammatory response, with effects on corneal wound healing and angiogenesis [33]. Gene silencing of NFκB was shown to impair in vitro wound healing by keratinocytes [34]; moreover, natural products also used for wound healing, were able to exert potent antiproliferative and antiangiogenic activity by blocking the NFκB cascade in different cell types, including endothelial cells [35]. The interconnection of inflammation and angiogenesis was also demonstrated for substances able to reduce NFκB activation by VEGF and to inhibit the angiogenic cascade both in vitro and in vivo [36].…”

Section: Discussionmentioning

confidence: 99%

Effect of Platelet Lysate on Human Cells Involved in Different Phases of Wound Healing

et al. 2013

View full text Add to dashboard Cite

BackgroundPlatelets are rich in mediators able to positively affect cell activity in wound healing. Aim of this study was to characterize the effect of different concentrations of human pooled allogeneic platelet lysate on human cells involved in the different phases of wound healing (inflammatory phase, angiogenesis, extracellular matrix secretion and epithelialization). Methodology/Principal FindingsPlatelet lysate effect was studied on endothelial cells, monocytes, fibroblasts and keratinocytes, in terms of viability and proliferation, migration, angiogenesis, tissue repair pathway activation (ERK1/2) and inflammatory response evaluation (NFκB). Results were compared both with basal medium and with a positive control containing serum and growth factors. Platelet lysate induced viability and proliferation at the highest concentrations tested (10% and 20% v/v). Whereas both platelet lysate concentrations increased cell migration, only 20% platelet lysate was able to significantly promote angiogenic activity (p<0.05 vs. control), comparably to the positive control. Both platelet lysate concentrations activated important inflammatory pathways such as ERK1/2 and NFκB with the same early kinetics, whereas the effect was different for later time-points. Conclusion/SignificanceThese data suggest the possibility of using allogeneic platelet lysate as both an alternative to growth factors commonly used for cell culture and as a tool for clinical regenerative application for wound healing.

show abstract

“…In previous studies, we found that Rottlerin, a natural polyphenol purified from the kamala powder [2], may act as an antitumor agent by a variety of mechanisms, such as Akt and ERK-independent cell cycle arrest in MCF-7 cells [3], functional suppression of the transcription factor NF κ B in MCF-7, HT-29 cells [4], and in HaCaT keratinocytes [5] and angiogenesis inhibition [6]. Several other studies have reported the anticancer potential of Rottlerin in different human malignant tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Alternative Pathways of Cancer Cell Death by Rottlerin: Apoptosis versus Autophagy

Torricelli

Salvadori

Valacchi

et al. 2012

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Since the ability of cancer cells to evade apoptosis often limits the efficacy of radiotherapy and chemotherapy, autophagy is emerging as an alternative target to promote cell death. Therefore, we wondered whether Rottlerin, a natural polyphenolic compound with antiproliferative effects in several cell types, can induce cell death in MCF-7 breast cancer cells. The MCF-7 cell line is a good model of chemo/radio resistance, being both apoptosis and autophagy resistant, due to deletion of caspase 3 gene, high expression of the antiapoptotic protein Bcl-2, and low expression of the autophagic Beclin-1 protein. The contribution of autophagy and apoptosis to the cytotoxic effects of Rottlerin was examined by light, fluorescence, and electron microscopic examination and by western blotting analysis of apoptotic and autophagic markers. By comparing caspases-3-deficient (MCF-73def) and caspases-3-transfected MCF-7 cells (MCF-73trans), we found that Rottlerin induced a noncanonical, Bcl-2-, Beclin 1-, Akt-, and ERK-independent autophagic death in the former- and the caspases-mediated apoptosis in the latter, in not starved conditions and in the absence of any other treatment. These findings suggest that Rottlerin could be cytotoxic for different cancer cell types, both apoptosis competent and apoptosis resistant.

show abstract

Rottlerin Exhibits Antiangiogenic Effects In vitro

Cited by 28 publications

References 36 publications

Rottlerin inhibits cell growth and invasion via down-regulation of Cdc20 in glioma cells

Rottlerin inhibits cell growth and invasion via down-regulation of Cdc20 in glioma cells

Effect of Platelet Lysate on Human Cells Involved in Different Phases of Wound Healing

Alternative Pathways of Cancer Cell Death by Rottlerin: Apoptosis versus Autophagy

Contact Info

Product

Resources

About