Rothia aerolata sp. nov., isolated from exhaust air of a pig barn

Kämpfer, Peter; Kleinhagauer, Tanita; Busse, Hans‐Jürgen; Klug, Kerstin; Jäckel, Udo; Glaeser, Stefanie P.

doi:10.1099/ijsem.0.001153

Cited by 11 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Rothia bacteria are all Gram-positive rods and to date 14 species have been identified ( https://www.bacterio.net/genus/rothia ): Rothia aeria [ 8 ] , Rothia aerolata [ 9 ], Rothia amarae [ 10 ] , Rothia arfidiae [ 11 ], Rothia dentocariosa [ 12 ], Rothia endophytica [ 13 ] , Rothia halotolerans [ 14 ], Rothia koreensis [ 14 ] , Rothia kristinae [ 14 ], Rothia marina [ 15 ] , Rothia mucilaginosa [ 16 ], Rothia nasimurium [ 16 ] , Rothia nasisuis [ 17 ] and Rothia terrae [ 18 ]. Furthermore, unclassified and uncultured Rothia genomes have been reconstructed from metagenomic data [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of Rothia species reveals diversity in novel biosynthetic gene clusters and ecological adaptation to different eukaryotic hosts and host niches

Oliveira

Baarlen

et al. 2022

Microbial Genomics

View full text Add to dashboard Cite

Rothia species are understudied members of the phylum Actinobacteria and prevalent colonizers of the human and animal upper respiratory tract and oral cavity. The oral cavity, including the palatine tonsils, is colonized by a complex microbial community, which compete for resources, actively suppress competitors and influence host physiology. We analysed genomic data from 43 new porcine Rothia isolates, together with 112 publicly available draft genome sequences of Rothia isolates from humans, animals and the environment. In all Rothia genomes, we identified biosynthetic gene clusters predicted to produce antibiotic non-ribosomal peptides, iron scavenging siderophores and other secondary metabolites that modulate microbe–microbe and potentially microbe–host interactions. In vitro overlay inhibition assays corroborated the hypothesis that specific strains produce natural antibiotics. Rothia genomes encode a large number of carbohydrate-active enzymes (CAZy), with varying CAZy activities among the species found in different hosts, host niches and environments. These findings reveal competition mechanisms and metabolic specializations linked to ecological adaptation of Rothia species in different hosts.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative genomics of Rothia species reveals diversity in novel biosynthetic gene clusters and ecological adaptation to different eukaryotic hosts and host niches

Oliveira

Baarlen

et al. 2022

Microbial Genomics

View full text Add to dashboard Cite

show abstract

“…The genus Rothia comprises 8 species (www.bacterio.net/rothia.html), Rothia aeria [1], Rothiaamarae [2], Rothia dentocariosa [3], Rothia mucilaginosa [4], Rothia nasimurium [4], Rothia terrae [5], Rothia endophytica [6], and Rothia saerolata [7], which were isolated from an air sample, sludge of a foul water sewer, a human oral cavity, a human pharynx, the nose of a healthy mouse, subtropical fields, plant tissues, and pig barn respectively. Among the genus Rothia, R. dentocariosa, R. mucilaginosa, and R. aeria are found in the oral cavity and pharynx of humans [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Novel Selective Medium for the Isolation of Rothia aeria, Which Is an Inhabitant of the Human Oral Cavity

Kobayashi¹,

Uchibori²,

Tsuzukibash³

et al. 2018

J Dents Dent Med

View full text Add to dashboard Cite

Roth aeria, Rothia dentocariosa, and Rothia mucilaginosa are isolated from the human oral cavity. Among them, R. aeria can cause severe systemic infection diseases (e.g., bronchitis, endocarditis, pneumonia, and sepsis). However, the veritable prevalence of this organism in the human oral cavity has not ever been known. Thus, the selective medium for the isolation and quantification of R. aeria is necessary to assess the prevalence of this organism, and to diagnose various R. aeria infection diseases. To investigate R. aeria distribution in oral cavities, a novel selective medium (RAESM) was developed for isolating and quantifying R. aeria. RAESM consists of sodium gluconate, tryptone, meat extract, sodium fluoride, acriflavin neutral, fosfomycin, lincomycin, colistin, aztreonam, and agar. Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 16S rDNA genes of R. aeria. The percentage of R. aeria in saliva samples collected from 20 subjects was examined. Moreover, we examined the antibiotic susceptibility of thirty isolates from six subjects. The average growth recovery of R. aeria on RAESM was 96.6% compared with that on Brain Heart Infusion supplemented with Yeast Extract (BHI-Y) agar. Growth of other representative oral bacteria, including other Rothia species, was remarkably inhibited on the selective medium. The PCR primers reacted to R. aeria and did not react to other Rothia species or representative oral bacteria. R. aeria was detected as 1.0% of the total bacteria, 5.9×10 7 CFU/ml, on BHI-Y agar in the oral cavities of all subjects. R. aeria isolates obtained in this study were susceptible to most antibiotics; however R. aeria isolates from one subject were highly resistant to erythromycin, lincomycin, and clindamycin. R. aeria may be a part of the normal flora in the human oral cavities. A novel selective medium, RAESM, was useful for isolating R. aeria. Moreover, it was indicated that RAESM was useful for diagnosing R. aeria infections.

show abstract

Rothia santali sp. nov., endophytic bacteria isolated from sandalwood (Santalum album L.) seedling

et al. 2022

View full text Add to dashboard Cite

A novel, 'mustard yellow' pigmented aerobic bacterial strain designated AR01 T was isolated from hypocotyl tissue of a sandalwood seedling from Bangalore, India. The 16S rRNA gene of strain AR01 T had the highest 98.97% sequence similarity with Rothia halotolerans YIM 90716 T (KCTC 19172) followed by Rothia kristinae PM 129 T (NBRC 15354 T ) (97.31%) and Rothia koreensis P31 T (JCM 15915) (97.11%), respectively. The strain AR01 T was coccoid-shaped, non-motile, non-spore-forming, oxidase-negative, and catalase-positive. The strain AR01 T has a genome size of 3.31 Mb containing 2993 protein-coding genes including 48 tRNA and 10 rRNAs spread across 84 contigs. The genomic DNA G + C content was 71.77 mol%. The calculated dDDH was 31.10%, and the OrthoANI value was 85.27% compared to its closest related type strain Rothia halotolerans YIM 90716 T . The predominant cellular fatty acids were C 16:0 iso (30.04%), C 15:0 anteiso (37.42%), and C 17:0 anteiso (21.78%). The strain AR01 T contains major polar lipids including diphosphatidylglycerol and phosphatidylglycerol. Based on the distinct physiological, biochemical characteristics and genotypic relatedness indicated that AR01 T represents a novel species of the genus Rothia, for which the name Rothia santali sp. nov. (Type strain AR01 T = MCC 4800 T = JCM 35593 T ) is proposed.The GenBank/EMBL/DDBJ accession number for the reference 16S rRNA gene sequences of the strain AR01 T is OM838448. The accession number of the whole-genome of AR01 T is JANAFB000000000

show abstract

Rothia aerolata sp. nov., isolated from exhaust air of a pig barn

Cited by 11 publications

References 31 publications

Comparative genomics of Rothia species reveals diversity in novel biosynthetic gene clusters and ecological adaptation to different eukaryotic hosts and host niches

Comparative genomics of Rothia species reveals diversity in novel biosynthetic gene clusters and ecological adaptation to different eukaryotic hosts and host niches

Novel Selective Medium for the Isolation of Rothia aeria, Which Is an Inhabitant of the Human Oral Cavity

Rothia santali sp. nov., endophytic bacteria isolated from sandalwood (Santalum album L.) seedling

Contact Info

Product

Resources

About