1994
DOI: 10.1128/jcm.32.5.1408-1409.1994
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Abstract: Rotavirus serotype G5 in fecal specimens of 38 Brazilian children with diarrhea was identified by PCR and enzyme immunoassays. The strains exhibited long RNA electropherotypes and either subgroup II or nonsubgroup I-nonsubgroup II specificities. Serotype G5 has been found in piglets and horses but not yet in humans.

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Cited by 188 publications
(82 citation statements)
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“…Based on accumulated evidence, it is clear that GARs can transfer between pigs and humans, making pigs a potential reservoir for the emergence of unusual or novel strains of human GARs [13][14][15][16][17][18]. In fact, the novel G26 strain we found was most similar to the human 57vp7w strain, which can also be considered to be a G26 strain.…”
Section: Discussionmentioning
confidence: 92%
See 1 more Smart Citation
“…Based on accumulated evidence, it is clear that GARs can transfer between pigs and humans, making pigs a potential reservoir for the emergence of unusual or novel strains of human GARs [13][14][15][16][17][18]. In fact, the novel G26 strain we found was most similar to the human 57vp7w strain, which can also be considered to be a G26 strain.…”
Section: Discussionmentioning
confidence: 92%
“…The zoonotic potential of animal GARs is a great concern [12]. GARs with unusual G genotypes commonly found in pigs and cattle have been detected in sporadic and epidemic cases of diarrhea in human populations [13][14][15][16][17][18]. A full genome-based genotyping system proposed by Matthijnssens et al revealed the existence of reassortant strains and the close relationship between human and animal strains [2,3].…”
Section: Introductionmentioning
confidence: 99%
“…Currently 14 G and 20 P types have been described. Globally, G1-G4 are the most common G types although there is a tremendous diversity as, for example, while G8 has caused 30% of gastro-enteritis in Malawi [Cunliffe et al, 2001a], G5 was the G type most often isolated in South America [Gouvea et al, 1994], and other novel serotypes (e.g., G9) have been reported worldwide recently [Ramachandran et al, 2000;Cunliffe et al, 2002b].…”
Section: Introductionmentioning
confidence: 99%
“…A semi-nested, multiplex PCR reaction was performed using primers targeting the genotype-specific regions of the VP4 and VP7 genes [20][21][22][23][24].…”
Section: Multiplex Semi-nested Pcrmentioning
confidence: 99%
“…Two μl (of a 1:100 dilution of the first round PCR product) was used as the template from the second round of amplification along with 10 μl of 5 × reaction buffer, 6 μl 25 mM MgCl 2 , 2 μl dNTP (1.25 mM final concentration), 0.5 μl DNA Taq polymerase (5 U/μl), 1 μl of the 5'-common forward primer (20 pM) and 1 μl each of primers specific for bovine G-types (G5, G6, G8, G10 and G11) as reported previously by Gouvea et al, [22,23]. Amplification was performed using modified conditions described by Falcone et al, [24].…”
Section: G-type Determinationmentioning
confidence: 99%