Rotavirus infection in children with acute gastroenteritis in Aurangabad, Central Maharashtra

Maher, Ganesh; Pradhan, Gauri; Shetty, Sushmitha A.; Ranshing, Sujata S.; Damle, Ajit; Chitambar, Shobha D.

doi:10.1007/s13312-016-0900-6

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…On G and P combined typing G3P[8] was the most common genotype (133; 51.9%), followed by G1P[8] (44; 17.18%); this is different from other studies from various parts of the country where G1P[8] was the most prevalent strain. 9 22 23 24 Though G1P[8] was the most common genotype in the majority of the country, G3P[8] is the emerging strains in this region, which is similar to prior studies from this part of the country that showed G3P[8] as the most prevalent strain. 15 16 Another study from this belt suggested G1P[8] to be the most prevalent strain.…”

Section: Discussionsupporting

confidence: 80%

Clinical Profile and Characterization of Rotavirus Strains in Under-Five Children with Diarrhea: A Hospital-Based Study from Eastern Odisha, India

Prusty

Choudhury

Akhila

et al. 2022

J Pediatr Infect Dis

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Objective Diarrheal diseases are one of the most common causes of hospitalization in children under five. Rotavirus is the most common cause of acute diarrhea in younger children, and the prevalence decreases rapidly with increasing age. The objective of the study was to estimate the burden of rotavirus infection in acute gastroenteritis among under-five children admitted to a tertiary care hospital in eastern Odisha, for the clinical profile and identity of the prevalent strains. Methods This was a prospective observational study linked to the National Rotavirus Surveillance Network (NRSN), where 720 under-five children with diarrhea were enrolled. In total, 675 stool samples of eligible candidates were sent for rotavirus isolation, and identification of strains was done by identifying VP7 (G-type) and VP4 (P-type) genes by reverse transcription polymerase chain reaction. Results Categorical variables were presented as frequency and percentage, and continuous variables were expressed as mean ± standard deviation. Rotavirus was detected in 256 (37.92%) samples. Males outnumbered females. The most common affected age group was 7 to 12 months, followed by 13 to 18 months. G3P[8] was the most prevalent strain in this study. Conclusion Children between the age of 7 and 18 months were most vulnerable to rotavirus infection. The most prevalent strain varies from one region to another and continuous surveillance is needed.

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Section: Discussionsupporting

confidence: 80%

Clinical Profile and Characterization of Rotavirus Strains in Under-Five Children with Diarrhea: A Hospital-Based Study from Eastern Odisha, India

Prusty

Choudhury

Akhila

et al. 2022

J Pediatr Infect Dis

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“…In the present study, overall 39.87% and 39.24% samples were found to be positive for rotavirus by PAGE and RT-PCR, respectively. An earlier study from Western India reported 47% of samples from unvaccinated children collected from Pune and 11.9% samples from Aurangabad to be positive for rotavirus ( Jain et al., 2016 ; Maher et al., 2016 ). Higher positivity was observed in Goa than Meghalaya.…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, the proportion of G3 strains (P[8] and P[6]) was significantly higher with the P[8] as the dominant genotype (80.3%) followed by P[6] (14.6%) among patients admitted to the hospital with severe dehydrating diarrhea ( Athiyyah et al., 2019 ) was observed in Indonesia. Limited studies on rotaviral genotypes in Eastern India revealed G1 (57%) as the most predominant type followed by mixed types (20%), G2 (13%) and G4 (9%) (NICED, Annual Report, 2002–03), while, G1P[8] genotype was frequently isolated from Western India ( Chitamber et al., 2012 ; Jain et al., 2016 ; Maher et al., 2016 ). However, shift in dominating strains from the G1P[8] to G3P[8] was observed in 2016 from Eastern part of India ( Banerjee et al., 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

Raorane

Dubal

Ghatak

et al. 2020

Heliyon

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Introduction Rotavirus is the leading cause of diarrhoea in young children in India, responsible for an estimated 21357 mean numbers of deaths in 2010. Various genotypes of rotaviruses evolved due to mutational changes have been recognized. In this study, we determined the genotypes of rotaviruses involved in diarrhea in Goa and Meghalaya states of India. Methods The dsRNA of rotaviruses was extracted from stool samples and detected by Ribonucleic Acid-Polyacrylamide gel electrophoresis (RNA PAGE) and Reverse transcription-polymerase Chain Reaction (RT-PCR) targeting the partial VP7 gene. The full length VP7 and partial VP4 genes of rotavirus strains were amplified by RT-PCR followed by nucleotide sequencing. The RotaC classification tool was used to determine the genotypes. Results The positivity of rotavirus by PAGE and RT-PCR was observed to be 43.10% and 39.65% in Goa and 38% and 36% in Meghalaya, respectively. Though long electrophoretic profile was appeared to be the most predominant rotavirus type in circulation in these two states, 96% of long and 84.61% short electropherotype profiles could be detected by RT-PCR. The dsRNA of rotavirus extracted from 36 samples could be transcribed and amplified by beg9end9 primers for G genotyping, while, 41 by con3con2 primers for P genotyping. G1P[8] and G1P[6] genotypes were commonly circulated in Goa and G1P[8] and G1P[4] genotypes in Meghalaya. On nucleotide analysis, 6 samples from Goa showed G1 genotype specificity, while, 3 showed P[8] specificity indicating the G1P[8] rotavirus circulating in Goa. In Meghalaya state, 3 strains showed P[8] and 2 showed P[4] genotype specificity. The majority of the G and P genotypes were closely related to each other and G1 genotypes appeared in two separate clusters, while, P[8] and P[4] appeared in the respective clusters. Conclusion The circulation of G1P[8], G1P[6] genotypes in Goa and the presence of G1P[8] and G1P[4] genotypes in Meghalaya was observed.

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Clinical Profile, Risk-Factors, and Outcome of Rotaviral Diarrhea and Non-rotaviral Diarrhea Among Under-Five Children at Cuttack, Odisha, India

et al. 2021

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Rotavirus infection in children with acute gastroenteritis in Aurangabad, Central Maharashtra

Abstract: Rotavirus diarrhea was caused mainly by G1P[8] strains during 2012-2013 in Aurangabad, Central Maharashtra, India.

Cited by 6 publications

References 7 publications

Clinical Profile and Characterization of Rotavirus Strains in Under-Five Children with Diarrhea: A Hospital-Based Study from Eastern Odisha, India

Clinical Profile and Characterization of Rotavirus Strains in Under-Five Children with Diarrhea: A Hospital-Based Study from Eastern Odisha, India

Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

Clinical Profile, Risk-Factors, and Outcome of Rotaviral Diarrhea and Non-rotaviral Diarrhea Among Under-Five Children at Cuttack, Odisha, India

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