Rotavirus Cell Entry

Iša, Pavel; Gutiérrez, Michelle; Arias, Carlos F.; López, Susana

doi:10.2217/17460794.3.2.135

Cited by 12 publications

(19 citation statements)

References 97 publications

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“…The other three virus strains tested depend on clathrin-mediated endocytosis (caveolin-dependent or macropinocytosis uptake were ruled out). The findings in this work could help to clarify the different, sometimes even contradictory results which have been reported in the literature when characterizing different rotavirus strains (22). For instance, different results have been reported for the role of low pH on entry and for the role of the Ca 2ϩ concentration necessary for viral uncoating.…”

mentioning

confidence: 61%

“…Using as a model MA104 cells and the simian rotavirus RRV, we have proposed that rotavirus cell entry is a complex multistep process that involves the two virus surface proteins and several cell receptors, including sialic acids, gangliosides, integrins ␣2␤1, ␣4␤1, ␣v␤3, and ␣x␤2, and the heat shock cognate protein hsc70 (22). We have also shown that depletion of cholesterol from the cellular membrane severely impairs the infectivity of rotavirus (19,50) and have suggested that sphingolipid-and cholesterol-enriched membrane lipid microdomains might be involved in rotavirus cell entry, since the virus and its receptors associate with these domains at early times during infection (23).…”

mentioning

confidence: 99%

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Different Rotavirus Strains Enter MA104 Cells through Different Endocytic Pathways: the Role of Clathrin-Mediated Endocytosis

Gutiérrez¹,

Iša²,

Martin³

et al. 2010

J Virol

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111

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Rotaviruses, the single most important agents of acute severe gastroenteritis in children, are nonenveloped viruses formed by a three-layered capsid that encloses a genome formed by 11 segments of double-stranded RNA. The mechanism of entry of these viruses into the host cell is not well understood. The best-studied strain, RRV, which is sensitive to neuraminidase (NA) treatment of the cells, uses integrins ␣2␤1 and ␣v␤3 and the heat shock protein hsc70 as receptors and enters MA104 cells through a non-clathrin-, non-caveolin-mediated pathway that depends on a functional dynamin and on the presence of cholesterol on the cell surface. In this work, using a combination of pharmacological, biochemical, and genetic approaches, we compared the entry characteristics of four rotavirus strains known to have different receptor requirements. We chose four rotavirus strains that represent all phenotypic combinations of NA resistance or sensitivity and integrin dependence or independence. We found that even though all the strains share their requirements for hsc70, dynamin, and cholesterol, three of them differ from the simian strain RRV in the endocytic pathway used. The human strain Wa, porcine strain TFR-41, and bovine strain UK seem to enter the cell through clathrin-mediated endocytosis, since treatments that inhibit this pathway block their infectivity; consistent with this entry route, these strains were sensitive to changes in the endosomal pH. The inhibition of other endocytic mechanisms, such as macropinocytosis or caveola-mediated uptake, had no effect on the internalization of the rotavirus strains tested here.

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mentioning

confidence: 61%

mentioning

confidence: 99%

Different Rotavirus Strains Enter MA104 Cells through Different Endocytic Pathways: the Role of Clathrin-Mediated Endocytosis

Gutiérrez¹,

Iša²,

Martin³

et al. 2010

J Virol

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111

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“…Several proteins participate in the process that enables rotaviruses to enter cells (Isa et al, 2008). However, until now, research on rotavirus receptor proteins has only been carried out on MA104 and Caco-2 cell lines using rotaviruses adapted to them (Isa et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Integrin αvβ3 has been found in MA104 and Caco-2 cells (Guerrero et al, 2000a;Isa et al, 2008) and it has also been proposed as a rotavirus coreceptor, together with integrins α4β1, and αxβ2 (Coulson et al, 1997;Ciarlet et al, 2001;Graham et al, 2006). Th e Hsc70 and integrin αvβ3 are known to be located in the lipid raft microdomains of the cytoplasmic membrane (Isa et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Rotavirus receptor proteins Hsc70 and integrin αvβ3 are located in the lipid microdomains of animal intestinal cells

Ca¹,

Lp²

2012

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Summary. -Several cell surface molecules such as integrins, sialic acid and Hsc70 have been reported to participate in the process of adsorption and penetration of rotaviruses into cells. Some of them have been found in susceptible cell lines but not in epithelial cells of natural hosts so that their real role in the infection process is unclear. In this study, we attempted to confi rm the presence of Hsc70 and integrin αvβ3 in the cytoplasmic membrane of isolated intestinal epithelial cells of pig, mouse and cow. Using immunocytochemistry, immunofl uorescence, co-immunoprecipitation, ELISA, Western blot analysis and fl ow cytometry we established that in these cells, (i) Hsc70 and integrin αvβ3 formed a complex in lipid raft microdomains of the cytoplasmatic membrane and (ii) Hsc70 levels increased aft er rotavirus infection. Th ese results indicate that these molecules act as receptors of rotaviruses in susceptible cells.

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“…The initial step in rotaviral infection is virus attachment, performed after several reactions between the viral surface spike protein VP4 and the respective receptors on the cell membrane of host cells, such as integrins (Coulson et al, 1997) and heat shock protein Hsc70 (Isa et al, 2008). Some strains of the virus require the presence of sialic acid on the cell surface for efficient binding, but the great majority (of animal or human origin) is sialic acid-independent (Ciarlet and Estes, 1999).…”

Section: Transmission Of the Virus And Patho-genesis Of The Infectionmentioning

confidence: 99%

Rotavirus infections in domestic animals

Chatzopoulos

Athanasiou

Spyrou

et al. 2017

J Hellenic Vet Med Soc

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ABSTRACT. Rotaviruses are major enteric pathogens of humans and a wide variety of animals. Rotavirus infections have a worldwide prevalence. The viral genome is composed of 11 double-stranded RNA segments with six structural and five or six non-structural proteins. Over 35,000 strains have been identified and classified into five main (A, B, C, D, E) and three additional tentative (F, G, H) serotype groups. A binary classification system has been proposed defining 'G' or 'P' types, with at least 27 G and 35 P genotypes reported thusfar. The virus is transmitted primarily by the faecal-oral and oral routes. After attachment, entry into the host cells occurs through direct entry, fusion or endocytosis. Main mechanisms of Rotavirus-induced diarrhoea involve extensive enterocyte losses and nutrient disdigestion and malabsorption. Clinical features of Rotavirus infections range from asymptomatic infections to fulminant disease leading to rapid death. In calves, lambs and kids, piglets and foals, salient sign of the disease is diarrhoea; diarrhoeic faeces are white, yellow or, in severe cases, blood-tinted or frank haemorrhagic. In dogs and cats, the infection occurs usually as self-limiting diarrhoea. Avian Rotavirus infections are characterized by enteritis, growth depression and/or growth retardation. Definitive diagnosis of the infection can only be achieved by laboratory tests, including electron microscopic examination, immunohistochemical examination, immunofluorescence, ELISA, latex agglutination and molecular techniques. There is no specific treatment against Rotavirus infections. Treatment is based in providing supportive care and managing clinical signs and potential complications. Effective vaccines, containing inactivated, recombinant or attenuated strains of the virus, are available. Challenge studies have shown the ease of the virus in cross-infecting various animal species; animal strains of the virus may cross species and infect humans. Due to the ability of the virus to overcome species barriers, animal strains may act as natural source of viral genomes, promoting mutations and creating new viral genotypes, whose virulence cannot be predictable.

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Rotavirus Cell Entry

Cited by 12 publications

References 97 publications

Different Rotavirus Strains Enter MA104 Cells through Different Endocytic Pathways: the Role of Clathrin-Mediated Endocytosis

Different Rotavirus Strains Enter MA104 Cells through Different Endocytic Pathways: the Role of Clathrin-Mediated Endocytosis

Rotavirus receptor proteins Hsc70 and integrin αvβ3 are located in the lipid microdomains of animal intestinal cells

Rotavirus infections in domestic animals

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