Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs

Miyaji, Hirofumi; Sugaya, Tsutomu; Ibe, Keisuke; Ishizuka, Ryousuke; Tokunaga, Ken; Kawanami, Masamitsu

doi:10.1111/j.1600-0765.2010.01281.x

Cited by 22 publications

(22 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, all groups employing the barrier membrane indicated more cementum regeneration than the control group. These findings were consistent with the previous studies (Miyaji et al, ; Ribeiro et al, ). Notably, the height of cementum was lower than the height of the bone in the BMP‐2/BioCaP + barrier membrane group.…”

Section: Discussionsupporting

confidence: 94%

Periodontal regeneration using bone morphogenetic protein 2 incorporated biomimetic calcium phosphate in conjunction with barrier membrane: A pre‐clinical study in dogs

Wei

Teng

Deng

et al. 2019

J Clinic Periodontology

View full text Add to dashboard Cite

AimTo evaluate the effect of bone morphogenetic protein 2 (BMP‐2) incorporated biomimetic calcium phosphate (BMP‐2/BioCaP) in conjunction with barrier membrane on periodontal regeneration in chronic periodontitis experimental model.Material and MethodsChronic periodontitis experimental model with critical‐sized supra‐alveolar defects was created in 15 dogs’ mandibles. After the initial periodontal therapy, the defects were randomly assigned to the following groups: (a) control; (b) barrier membrane; (c) deproteinized bovine bone mineral + barrier membrane; (d) BioCaP + barrier membrane and (e) BMP‐2/BioCaP + barrier membrane (6 quadrants with 18 teeth per group). Eight weeks later, clinical examinations, micro‐CT, and histomorphometric analyses were performed.ResultsClinical examinations, including plaque index, bleeding index, and probing depth, were similar for all groups. In contrast, the clinical attachment loss was significantly lower in defects grafted with BMP‐2/BioCaP and barrier membrane. The micro‐CT results showed that the height of mineralized tissue in defects grafted with BMP‐2/BioCaP and barrier membrane was significantly higher. For histometric analysis, the defects grafted with BMP‐2/BioCaP and barrier membrane exhibited significantly more connective tissue height, new cementum height, new bone height and area, as well as less down‐growth of junctional epithelium.ConclusionBMP‐2/BioCaP could be a promising bone substitute for periodontal regeneration.

show abstract

Section: Discussionsupporting

confidence: 94%

Periodontal regeneration using bone morphogenetic protein 2 incorporated biomimetic calcium phosphate in conjunction with barrier membrane: A pre‐clinical study in dogs

Wei

Teng

Deng

et al. 2019

J Clinic Periodontology

View full text Add to dashboard Cite

show abstract

“…self‐reorganization) is extremely difficult to control completely. For example, bone morphogenetic protein strongly promotes bone augmentation, but regeneration of the periodontal ligament is rarely observed, and ankylosis is frequently detected . In contrast, creation of fibrous tissue, such as periodontal membrane, and the absence of ankylosis were apparent in this study.…”

Section: Discussionmentioning

confidence: 51%

Periodontal tissue engineering by nano beta‐tricalcium phosphate scaffold and fibroblast growth factor‐2 in one‐wall infrabony defects of dogs

Ogawa

Miyaji

Kato

et al. 2016

J of Periodontal Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…38,39 The application of nanoparticle-modified structures might increase the surface area and subsequent signaling molecule adsorption, thereby improving cell behaviors. [10][11][12]40 The present SEM observation in 1 µg/mL GO scaffold demonstrated that wrinkling structures covered by GO sheets were formed frequently on the collagen strut of the scaffold. The GO sheet possesses many functional groups including hydroxyl (OH), epoxy (C-O-C), and carboxyl (COOH) species on its surface.…”

mentioning

confidence: 51%

Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket

Nishida¹,

Miyaji²,

Kato³

et al. 2016

IJN

View full text Add to dashboard Cite

Graphene oxide (GO) consisting of a carbon monolayer has been widely investigated for tissue engineering platforms because of its unique properties. For this study, we fabricated a GO-applied scaffold and assessed the cellular and tissue behaviors in the scaffold. A preclinical test was conducted to ascertain whether the GO scaffold promoted bone induction in dog tooth extraction sockets. For this study, GO scaffolds were prepared by coating the surface of a collagen sponge scaffold with 0.1 and 1 μg/mL GO dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), physical testing, cell seeding, and rat subcutaneous implant testing. Then a GO scaffold was implanted into a dog tooth extraction socket. Histological observations were made at 2 weeks postsurgery. SEM observations show that GO attached to the surface of collagen scaffold struts. The GO scaffold exhibited an interconnected structure resembling that of control subjects. GO application improved the physical strength, enzyme resistance, and adsorption of calcium and proteins. Cytocompatibility tests showed that GO application significantly increased osteoblastic MC3T3-E1 cell proliferation. In addition, an assessment of rat subcutaneous tissue response revealed that implantation of 1 μg/mL GO scaffold stimulated cellular ingrowth behavior, suggesting that the GO scaffold exhibited good biocompatibility. The tissue ingrowth area and DNA contents of 1 μg/mL GO scaffold were, respectively, approximately 2.5-fold and 1.4-fold greater than those of the control. Particularly, the infiltration of ED2-positive (M2) macrophages and blood vessels were prominent in the GO scaffold. Dog bone-formation tests showed that 1 μg/mL GO scaffold implantation enhanced bone formation. New bone formation following GO scaffold implantation was enhanced fivefold compared to that in control subjects. These results suggest that GO was biocompatible and had high bone-formation capability for the scaffold. The GO scaffold is expected to be beneficial for bone tissue engineering therapy

show abstract

Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs

Cited by 22 publications

References 24 publications

Periodontal regeneration using bone morphogenetic protein 2 incorporated biomimetic calcium phosphate in conjunction with barrier membrane: A pre‐clinical study in dogs

Periodontal regeneration using bone morphogenetic protein 2 incorporated biomimetic calcium phosphate in conjunction with barrier membrane: A pre‐clinical study in dogs

Periodontal tissue engineering by nano beta‐tricalcium phosphate scaffold and fibroblast growth factor‐2 in one‐wall infrabony defects of dogs

Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket

Contact Info

Product

Resources

About