Roles of plant growth substance in callus induction of Achyranthes bidentata

HongYing, Duan; Ding, Weikai; Jian-ying, Song; Xu, Jiaming; Wang, Huina; Zhu, Yanqiu; Liu, Wenxiao; Zhou, Yanqing

doi:10.19071/ripb.2016.v6.3079

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Tsatsai [ 37 ] but in contrast to the majority of previous studies which found proper exogenous auxin promotes rooting of in vitro shoots [ 35 ]. It is commonly accepted that exogenous auxin plays a key role for plant callus induction and proliferation, however, exogenous cytokinin, may play a coordinating role for callus induction and proliferation [ 35 , 38 ]. In addition, proliferation of habituated Arabidopsis callus is dependent on exogenous auxin but not on cytokinin [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

A novel micropropagation of Lycium ruthenicum and epigenetic fidelity assessment of three types of micropropagated plants in vitro and ex vitro

Gao

Wang

et al. 2021

PLoS ONE

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Lycium ruthenicum is an excellent eco-economic shrub. Numerous researches have been conducted for the function of its fruits but scarcely focused on the somaclonal variation and DNA methylation. An efficient micropropagation protocol from leaves and stems of L. ruthenicum was developed in this study, in which not only the leaf explants but also the stem explants of L. ruthenicum were dedifferentiated and produced adventitious buds/multiple shoots on one type of medium. Notably, the efficient indirect organogenesis of stem explants was independent of exogenous auxin, which is contrary to the common conclusion that induction and proliferation of calli is dependent on exogenous auxin. We proposed that sucrose supply might be the crucial regulator of stem callus induction and proliferation of L. ruthenicum. Furthermore, results of methylation-sensitive amplified polymorphism (MSAP) showed that DNA methylation somaclonal variation (MSV) of CNG decreased but that of CG increased after acclimatization. Three types of micropropagated plants (from leaf calli, stem calli and axillary buds) were epigenetically diverged more from each other after acclimatization and the ex vitro micropropagated plants should be selected to determine the fidelity. In summary, plants micropropagated from axillary buds and leaves of L. ruthenicum was more fidelity and might be suitable for preservation and propagation of elite germplasm. Also, leaf explants should be used in transformation. Meanwhile, plants from stem calli showed the highest MSV and might be used in somaclonal variation breeding. Moreover, one MSV hotspot was found based on biological replicates. The study not only provided foundations for molecular breeding, somaclonal variation breeding, preservation and propagation of elite germplasm, but also offered clues for further revealing novel mechanisms of both stem-explant dedifferentiation and MSV of L. ruthenicum.

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Section: Discussionmentioning

confidence: 99%

A novel micropropagation of Lycium ruthenicum and epigenetic fidelity assessment of three types of micropropagated plants in vitro and ex vitro

Gao

Wang

et al. 2021

PLoS ONE

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“…The bud formation from callus was highly influenced by auxins 2,4-D, NAA and IBA. The effect of 2,4-D was also significant for root induction (Duan et al 2012 ). The aim of the studies carried out by Sen et al ( 2014 ) was to regenerate the plantlets of A. aspera via indirect organogenesis.…”

Section: Ecdysteroid Production In Plant In Vitro Culturesmentioning

confidence: 99%

Ecdysteroids: production in plant in vitro cultures

et al. 2016

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Ecdysteroids are secondary metabolites, widely distributed in the animal and plant kingdoms. They have a wide range of pharmacological effects in vertebrates, including mammals, most of which are beneficial for humans. Therefore, they have become compounds of interest for the pharmaceutical industry due to their adaptogenic, anabolic, hypoglycaemic, hypocholesterolaemic and antimicrobial activities, which are still being researched. Nowadays, ecdysteroids are present as active ingredients in bodybuilding supplements. Because of their complex structures, their chemical synthesis seems unprofitable and impractical. Due to high content of ecdysteroids in many plants, they are primarily obtained by extraction of the plant material. Plant in vitro cultures provide an alternative source of these compounds, helping to avoid problems associated with field production—such as variable yield or dependence on environmental factors, as well as limited availability of natural resources. Plant cell and tissue cultures may be suggested as alternatives for the production of plant biomass rich in pharmaceutically active ecdysteroids. Moreover, the use of common biotechnological strategies, such as elicitation or precursor feeding, may further increase the yield and improve production of these compounds. In this paper, we describe general information about ecdysteroids: their structure, biosynthesis, distribution, role in plants, and we review recent studies on micropropagation of ecdysteroid-producing plants and cell cultures, and potential ability of ecdysteroids enhancement in in vitro cultures.

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Roles of plant growth substance in callus induction of Achyranthes bidentata

Cited by 2 publications

References 7 publications

A novel micropropagation of Lycium ruthenicum and epigenetic fidelity assessment of three types of micropropagated plants in vitro and ex vitro

A novel micropropagation of Lycium ruthenicum and epigenetic fidelity assessment of three types of micropropagated plants in vitro and ex vitro

Ecdysteroids: production in plant in vitro cultures

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