Roles of ABF1, NPL3, and YCL54 in silencing in Saccharomyces cerevisiae.

Loo, Stephen; Laurenson, Patricia; Foss, Margit; Dillin, Andrew; Rine, Jasper

doi:10.1093/genetics/141.3.889

Cited by 61 publications

(7 citation statements)

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“…To the right of α2#2 is an essential gene, YCL054 . It has been identified as somehow being implicated in silencing (Loo et al ., 1995).…”

Section: Resultsmentioning

confidence: 99%

“…Thus, in α‐cells, an organized chromatin domain spanning 3.7 kb exists over and surrounding the RE region; a summary of all the data in α‐cells is shown in Figure 7. On both sides, the boundary of that domain is represented by the promoter region of genes YCL056 (Defoor et al ., 1992), to the left of α2#1, and YCL054 , an essential gene (Loo et al ., 1995), to the right of α2#2. Chromatin in tup1 mutant α‐cells is disrupted over at least the right portion of the domain (data not shown).…”

Section: Resultsmentioning

confidence: 99%

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Cell type-specific chromatin organization of the region that governs directionality of yeast mating type switching

Weiß

Simpson

1997

The EMBO Journal

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Switching of mating type in Saccharomyces cerevisiae is directional; MATα cells recombine to transfer information from HMRa while MATa cells switch using the silent cassette at HMLα. Genetic analysis recently has defined a 700 bp recombination enhancer ∼29 kb from the left end of chromosome III that is necessary for directionality. The chromatin structure of this region differs strikingly in a‐ and α‐cells. Matα2p organizes a 3.7 kb chromatin domain that opposes interaction of trans‐acting proteins with the enhancer. In a‐cells lacking the α2 repressor, two footprinted regions flank an ∼100 bp section having a unique DNA structure. This structural signature probably reflects interactions of proteins that result in directional mating type switching.

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“…To the right of α2#2 is an essential gene, YCL054 . It has been identified as somehow being implicated in silencing (Loo et al ., 1995).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Cell type-specific chromatin organization of the region that governs directionality of yeast mating type switching

Weiß

Simpson

1997

The EMBO Journal

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“…Construction of SY77. The HMR a gene of IH2531 ( ␣ ura3-52 ) was disrupted by introduction of pDR58 digested with EcoRV (Loo et al, 1995), creating SY77. The disruption was confirmed by Southern blot analysis.…”

Section: Isolation Of Axial-defective Mutantsmentioning

confidence: 99%

O-Glycosylation of Axl2/Bud10p by Pmt4p Is Required for Its Stability, Localization, and Function in Daughter Cells

Sanders

Gentzsch

Tanner

et al. 1999

The Journal of Cell Biology

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Cells of the yeast Saccharomyces cerevisiae choose bud sites in a manner that is dependent upon cell type: a and α cells select axial sites; a/α cells utilize bipolar sites. Mutants specifically defective in axial budding were isolated from an α strain using pseudohyphal growth as an assay. We found that a and α mutants defective in the previously identified PMT4 gene exhibit unipolar, rather than axial budding: mother cells choose axial bud sites, but daughter cells do not. PMT4 encodes a protein mannosyl transferase (pmt) required for O-linked glycosylation of some secretory and cell surface proteins (Immervoll, T., M. Gentzsch, and W. Tanner. 1995. Yeast. 11:1345–1351). We demonstrate that Axl2/Bud10p, which is required for the axial budding pattern, is an O-linked glycoprotein and is incompletely glycosylated, unstable, and mislocalized in cells lacking PMT4. Overexpression of AXL2 can partially restore proper bud-site selection to pmt4 mutants. These data indicate that Axl2/Bud10p is glycosylated by Pmt4p and that O-linked glycosylation increases Axl2/ Bud10p activity in daughter cells, apparently by enhancing its stability and promoting its localization to the plasma membrane.

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“…C) Quantitative mating of the wild type HMR cassette located at LEU2. Quantitative mating analyses of various regulatory elements driving expression of MATa1 were performed as described previously [126][127][128] . The data are presented as diploid CFU and are mean values from three independent experiments carried out in parallel.…”

Section: Bistability Versus Monostabilitymentioning

confidence: 99%

Yeast Heterochromatin Only Stably Silences Weak Regulatory Elements by Altering Burst Duration

Wu,

Dhillon,

Bajor

et al. 2023

Preprint

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The interplay between nucleosomes and transcription factors leads to programs of gene expression. Transcriptional silencing involves the generation of a chromatin state that represses transcription and is faithfully propagated through DNA replication and cell division. Using multiple reporter assays, including directly visualizing transcription in single cells, we investigated a diverse set of UAS enhancers and core promoters for their susceptibility to heterochromatic gene silencing. These results show that heterochromatin only stably silences weak and stress induced regulatory elements but is unable to stably repress housekeeping gene regulatory elements and the partial repression did not result in bistable expression states.Permutation analysis of different UAS enhancers and core promoters indicate that both elements function together to determine the susceptibility of regulatory sequences to repression. Specific histone modifiers and chromatin remodellers function in an enhancer specific manner to aid these elements to resist repression suggesting that Sir proteins likely function in part by reducing nucleosome mobility. We also show that the strong housekeeping regulatory elements can be repressed if silencer bound Sir1 is increased, suggesting that Sir1 is a limiting component in silencing.Together, our data suggest that the heterochromatic locus has been optimized to stably silence the weak mating type gene regulatory elements but not strong housekeeping gene regulatory sequences which could help explain why these genes are often found at the boundaries of silenced domains.

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Roles of ABF1, NPL3, and YCL54 in silencing in Saccharomyces cerevisiae.

Cited by 61 publications

References 0 publications

Cell type-specific chromatin organization of the region that governs directionality of yeast mating type switching

Cell type-specific chromatin organization of the region that governs directionality of yeast mating type switching

O-Glycosylation of Axl2/Bud10p by Pmt4p Is Required for Its Stability, Localization, and Function in Daughter Cells

Yeast Heterochromatin Only Stably Silences Weak Regulatory Elements by Altering Burst Duration

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