Role of the template layer in organizing self-assembled films: zirconium phosphonate monolayers and multilayers at a Langmuir-Blodgett template

Byrd, Houston; Whipps, Scott; Pike, John K.; Ma, Jingfei; Nagler, S. E.; Talham, Daniel R.

doi:10.1021/ja00080a034

Cited by 107 publications

(148 citation statements)

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“…An inorganic system that we have found to meet these requirements is a series of thin films of metal organophosphonates and organophosphates. [41][42][43][44][45][46][47] In the solid-state, many examples form layered structures with organic sheets separating continuous inorganic networks of metal ions coordinated by the phosphonate or phosphate oxygen atoms. [48] An example is the layered Zr(O 3 PC 6 H 5 ) 2 shown in Figure 4.…”

Section: Metal Phosphonate Modified Slidesmentioning

confidence: 99%

“…2005, 11, 1980 -1988 www.chemeurj.org 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim ated on hydrophobic supports by using Langmuir-Blodgett (LB) methods. [41][42][43][44][45][46] The LB process begins with an octadecylphosphonic acid (ODPA) Langmuir monolayer that is deposited onto the hydrophobic solid support in such a way that the hydrophilic acid group (PO 3 H 2 ) is directed away from the support (Figure 7). [41,42] The substrate is then removed from the LB trough and exposed to a solution of Zr 4 + ions that bind to give a monolayer of the zirconated octadecylphosphonic acid (ODPA-Zr).…”

Section: Metal Phosphonate Modified Slidesmentioning

confidence: 99%

“…The same situation arises in the zirconated LB films. The strongly binding zirconium ions cross-link the original monolayer, providing a well-defined interface of zirconium phosphonate sites [41,42] that sticks strongly to the surface, because it is no longer a traditional LB film of individual molecules physisorbed to the surface but rather a network or monolayer tape in which adhesion comes from the sum of all molecules in a cross-linked array. The zirconium phosphonate films are not soluble in organic solvents, and dissolve in water only below pH 1.…”

Section: Metal Phosphonate Modified Slidesmentioning

confidence: 99%

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Metal Phosphonates Applied to Biotechnologies: A Novel Approach to Oligonucleotide Microarrays

Bujoli

Lane

Nonglaton

et al. 2005

Chemistry A European J

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A new process for preparing oligonucleotide arrays is described that uses surface grafting chemistry which is fundamentally different from the electrostatic adsorption and organic covalent binding methods normally employed. Solid supports are modified with a mixed organic/inorganic zirconium phosphonate monolayer film providing a stable, well-defined interface. Oligonucleotide probes terminated with phosphate are spotted directly on to the zirconated surface forming a covalent linkage. Specific binding of terminal phosphate groups with minimal binding of the internal phosphate diesters has been demonstrated. The mixed organic/inorganic thin films have also been extended for use arraying DNA duplex probes, and therefore represent a viable general approach to DNA-based bioarrays. Ideas for interfacing mixed organic/inorganic interfaces to other bioapplications are also discussed.

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Section: Metal Phosphonate Modified Slidesmentioning

confidence: 99%

Section: Metal Phosphonate Modified Slidesmentioning

confidence: 99%

Section: Metal Phosphonate Modified Slidesmentioning

confidence: 99%

See 1 more Smart Citation

Metal Phosphonates Applied to Biotechnologies: A Novel Approach to Oligonucleotide Microarrays

Bujoli

Lane

Nonglaton

et al. 2005

Chemistry A European J

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“…Monolith with 150 s grafting gave similar result. It may suggest that each Zr 41 would bind one phosphate site (with two negative charges) in stoichiometry, resulting in opposite but equal charge on monolith surface after chelation [44]. However, the EOF mobility for 300-s grafting column was about 40% higher in magnitude after Zr 41 binding, indicating a higher charge density on porous surface of Zr 41 -monolith after chelating of Zr 41 .…”

Section: Immobilization and Regeneration Of Zr 41mentioning

confidence: 99%

Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides

Wang

Duan

et al. 2011

J of Separation Science

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To meet the demands of protein phosphorylation study, immobilized zirconium ion affinity chromatography (Zr 41 -IMAC) monolith was prepared by combining UV-initiated polymerization of monolithic support and subsequent photografting in both capillary columns and microchannels. Hydrophilic poly(2-hydroxyethyl methacrylate (HEMA)-coethylene dimethacrylate (EDMA)) monolithic support was prepared under UV irradiation at the wavelength of 365 nm with monomer HEMA, crosslinker EDMA and 2,2-dimethoxy-2-phenylacetophenone as photoinitiator in 1-decanol solution, which provides good biocompatibility and permeability for biomolecule analysis. To introduce chelating ligands, such as phosphate groups, on the pore surface of monolith for metal ion immobilization, photografting of ethylene glycol methacrylate phosphate with benzophenone as the photoinitiator was performed at 254 nm for 300 s. The grafting process and metal ion immobilization can be monitored by measuring the electroosmotic flow produced by the modified monolith, providing a quantitative evaluation of post-modification. This new method for the preparation of Zr 41 -IMAC monolith simplifies the optimization of monolith preparation and avoids the time-consuming chemical modification process. Additionally, advantages include facile preparation in microdevices, easy regenerability and good reproducibility. After optimization, the microchip-based Zr 41 -IMAC monolith was used for phosphopeptide analysis and showed good selectivity in phosphopeptide enrichment with matrix-assisted laser desorption ionization mass spectrometry detection. IntroductionIn recent years, protein phosphorylation has gained much attention because of its significant roles in cellular signal transduction processes [1,2]. However, the amount of phosphorylated proteins in eukaryotic cells often exists in substoichiometry level, which makes the identification of phosphorylation sites challenging [3]. To date, mass spectrometry (MS) analysis has become an effective and commonly used method for characterization of phosphoproteins [4]. To reduce the interference of non-phosphopeptides, selective enrichment strategies, such as immobilized metal ion affinity chromatography (IMAC) [5][6][7], metal oxide affinity chromatography (MOAC) [8,9], and ion exchange chromatography [10][11][12][13], are necessary before MS analysis.Recently, chip-based system has been introduced as a promising platform for phosphopeptide analysis, due to the benefits of low sample amount, high throughput, and easy integration [14]. Several microfluidic devices have been developed by integrating phosphopeptide enrichment with other functions, such as protein alkylation and reduction [15], protein digestion [16], peptide separation and on-line interface for MS instrument [17][18][19][20], which have shown great potentials in the field of phosphoproteome research. However, in these microchip devices, phosphopeptide enrichment components are usually fabricated by packing functionalized particles, such as TiO 2 or IMAC beads, into m...

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“…We expect that DMPA will form a strong complex with Zr 4+ based on a wealth of literature covering the formation of Zrphosphates and phosphonates [3,[12][13][14][15][16][17][18][19][20][21][22][23][25][26][27][28][29]31,[34][35][36][37][38]. For the other phospholipid head groups, intuition suggests that steric considerations would preclude the efficient formation of a ZP complex with a phospholipid.…”

Section: Resultsmentioning

confidence: 99%

Strategies for the growth of self-assembled phospholipid adlayers

Oberts¹,

Blanchard²

2010

Bioelectrochemistry

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Role of the template layer in organizing self-assembled films: zirconium phosphonate monolayers and multilayers at a Langmuir-Blodgett template

Cited by 107 publications

References 0 publications

Metal Phosphonates Applied to Biotechnologies: A Novel Approach to Oligonucleotide Microarrays

Metal Phosphonates Applied to Biotechnologies: A Novel Approach to Oligonucleotide Microarrays

Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides

Strategies for the growth of self-assembled phospholipid adlayers

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