Role of the Interstitial Cells Distributed in the Myenteric Plexus in Neural Reflexes in the Mouse Ileum

Fujita, Akikazu; Okishio, Yutaka; Fujinami, Kaori; Nakagawa, Masashi; Takeuchi, Tadayoshi; Takewaki, Tadashi; Hata, Fumiaki

doi:10.1254/jphs.fp0040499

Cited by 11 publications

(4 citation statements)

References 41 publications

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“…Similarly, the tone of the internal anal sphincter was shown to be significantly lower in W/W v mice compared with wild type (12.8 vs. 9.5 mmHg) (31), although no significant difference was found in another study (21 vs. 15 mmHg) (8). The tone in the intestinal musculature of W/W v mice appears to be reduced (14). Although eNOS has been suggested to generate NO for regulation of sphincter tone, eNOS is unlikely to be different in wild-type and W/W v mice and may not contribute to the differences between wild-type and W/W v mice (31).…”

Section: Discussionmentioning

confidence: 86%

Interstitial cells of Cajal and adaptive relaxation in the mouse stomach

Dixit¹,

Zárate²,

Liu³

et al. 2006

American Journal of Physiology-Gastrointestinal and Liver Physi

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Interstitial cells of Cajal (ICC) are proposed to play a role in stretch activation of nerves and are under intense investigation for potential roles in enteric innervation. Most data to support such roles come from in vitro studies with muscle strips whereas data at the whole organ level are scarce. To obtain insight into the role of ICC in distention-induced motor patterns developing at the organ level, we studied distension-induced adaptive relaxation in the isolated whole stomach of wild-type and W/W(v) mice. A method was developed to assess gastric adaptive relaxation that gave quantitative information on rates of pressure development and maximal adaptive relaxation. Pressure development was monitored throughout infusion of 1 ml of solution over a 10-min period. The final intraluminal pressure was sensitive to blockade of nitric oxide synthase, in wild-type and W/W(v) mice to a similar extent, indicating NO-mediated relaxation in W/W(v) mice. Adaptive relaxation occurred between 0.2 and 0.5 ml of solution infusion; this reflex was abolished by TTX, was not sensitive to blockade of nitric oxide synthase, but was abolished by apamin, suggesting that ATP and not nitric oxide is the neurotransmitter responsible for this intrinsic reflex. Despite the absence of intramuscular ICC (ICC-IM), normal gastric adaptive relaxation occurred in the W/W(v) stomach. Because pressure development was significantly lower in W/W(v) mice compared with wild type in all the conditions studied, including in the presence of TTX, ICC-IM may play a role in development of myogenic tone. In conclusion, a mouse model was developed to assess the intrinsic component of gastric accommodation. This showed that ICC-IM are not essential for activation of intrinsic sensory nerves nor ATP-driven adaptive relaxation nor NO-mediated relaxation in the present model. ICC-IM may be involved in regulation of (distention-induced) myogenic tone.

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Section: Discussionmentioning

confidence: 86%

Interstitial cells of Cajal and adaptive relaxation in the mouse stomach

Dixit¹,

Zárate²,

Liu³

et al. 2006

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Within the intestinal phase of digestion, a series of reflexes act to control the distal and proximal motility of the gut (Brownlee, 2011). The myenteric reflex, driven by local distension of the gut wall by a bolus of digesta, results in a local increase in the number and strength of contractions mediated by the myenteric plexus within the smooth muscle layers (Fujita, Okishio, Fujinami, Nakagawa, Takeuchi, Takewaki, et al, 2004). The myenteric reflex is initiated by the interstitial cells of Cajal and is driven by release of acetylcholine (Klein, Seidler, Kettenberger, Sibaev, Rohn, Feil, et al, 2013), leading to the orchestration of contraction and relaxation events in the immediate vicinity.…”

Section: Dietary Fibres and The Commencement Of The Intestinal Phase mentioning

confidence: 99%

The impact of dietary fibres on the physiological processes governing small intestinal digestive processes

Chater

Wilcox

Pearson

et al. 2015

Bioactive Carbohydrates and Dietary Fibre

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“…The myenteric neurones have been identified and categorized according to their functional properties and neurochemical, morphometric and quantitative characteristics, which demonstrate the great neuronal variability existing in the MP among animal species and in different segments of the digestive tube (Balemba et al., 1999; Brehmer et al., 1999; Costa et al., 2000; Furness, 2000; Brookes, 2001; Hansen, 2003; Bornstein et al., 2004; Fujita et al., 2004).…”

Section: Introductionmentioning

confidence: 99%

Quantification and Morphometry of Myenteric Neurones in the Jejunum of Holtzman Rats (Rattus norvegicus)

Mari

Scoz

Germano

et al. 2011

Anatomia, Histologia, Embryologia

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The morphological pattern of the myenteric plexus (MP) is species-specific, and little is known about this pattern in Holtzman rats. The aim of the current experiment was the morphological and quantitative study of myenteric neurones in the Holtzman rat jejunum. Hematoxylin-Eosin and NADH-diaphorase (NADH-dp) staining were used to assess muscular layer thickness, neurone cell body area (CBA) and nuclei area (NA). Muscular layer thickness was found to be 114.77 ± 14.89 μm. Neuronal densities across the subregions of the jejunum were similar: mesenteric, 11.78 ± 2.89/mm(2) ; intermediate, 12.06 ± 2.69/mm(2) ; and antimesenteric, 10.67 ± 1.89/mm(2) . As expected, there was positive correlation between the CBA and NA of 79.19, 79.26 and 78.5% in the mesenteric, intermediate and antimesenteric subregions of the jejunum, respectively. Medium-sized neurones predominated in the ganglionic arrangement of the MP. These results indicate that the NADH-dp myenteric neurones in the jejunum of Holtzman rats are similar in many aspects to those found in the ileum of Holtzman rats and to those found in the small intestine of Wistar rats, including their location, ganglionic disposition and predominance of medium-sized CBA. However, neuronal density in the jejunum is lower than in the ileum. Based on these results showing morphological similarities to the MP of the Wistar rat, the Holtzman strain can be used to investigate the effects of adverse conditions on the morphology of the MP.

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Role of the Interstitial Cells Distributed in the Myenteric Plexus in Neural Reflexes in the Mouse Ileum

Cited by 11 publications

References 41 publications

Interstitial cells of Cajal and adaptive relaxation in the mouse stomach

Interstitial cells of Cajal and adaptive relaxation in the mouse stomach

The impact of dietary fibres on the physiological processes governing small intestinal digestive processes

Quantification and Morphometry of Myenteric Neurones in the Jejunum of Holtzman Rats (Rattus norvegicus)

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