Role of the 9-O-acetyl GD3 in subventricular zone neuroblast migration

Miyakoshi, L.M.; Todeschini, Adriane R.; Mendez-Otero, Rosália; Hedin-Pereira, Cecília

doi:10.1016/j.mcn.2011.08.010

Cited by 18 publications

(15 citation statements)

References 47 publications

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“…Also, in a previous study (Miyakoshi et al, 2012) we observed that the immunoblockade of the 9-O-acetyl GD3 ganglioside, a glycolipid GPI anchored to the plasma membrane, caused effects to the neuroblast migration that are similar to the ones described in this study. We detected the same formation of cellular clusters, decrease of cell mobility, impaired distinction of individual cells within chains and retraction of these chains back to the explants.…”

Section: Discussionsupporting

confidence: 88%

“…In most cases, the distal chains which shoot out at 1 DIV were no longer detectable; the migratory halos presented a compact organization, often forming clusters in the proximity of the explants (arrows in Figures 3A,B). We then quantified the effects of the different treatments on the migratory halos as described in Miyakoshi et al (2012). …”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have shown that common mechanisms can regulate both neuronal and cancer cell migration, such as metalloproteinases (Lee et al, 2006; Akter et al, 2015), gangliosides (Miyakoshi et al, 2012; Ohkawa et al, 2015), tyrosine kinase, and integrin receptors (Anton et al, 2004; Sidera et al, 2008). A variety of chaperones and associated proteins have already been implicated in the regulation of cell migration (Sims et al, 2011; El Hamidieh et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Similar to Miyakoshi et al (2012), P0-P7 mice were sacrificed and brains removed from the head. Parasagittal slices 350 μm thick were obtained with a tissue chopper (Mcllwain) and anterior SVZ was dissected out under scope inspection and were further cut into small pieces of approximately 100 μm diameter.…”

Section: Methodsmentioning

confidence: 99%

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Evidence of a Cell Surface Role for Hsp90 Complex Proteins Mediating Neuroblast Migration in the Subventricular Zone

Miyakoshi

Marques‐Coelho

Souza

et al. 2017

Front. Cell. Neurosci.

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In most mammalian brains, the subventricular zone (SVZ) is a germinative layer that maintains neurogenic activity throughout adulthood. Neuronal precursors arising from this region migrate through the rostral migratory stream (RMS) and reach the olfactory bulbs where they differentiate and integrate into the local circuitry. Recently, studies have shown that heat shock proteins have an important role in cancer cell migration and blocking Hsp90 function was shown to hinder cell migration in the developing cerebellum. In this work, we hypothesize that chaperone complexes may have an important function regulating migration of neuronal precursors from the subventricular zone. Proteins from the Hsp90 complex are present in the postnatal SVZ as well as in the RMS. Using an in vitro SVZ explant model, we have demonstrated the expression of Hsp90 and Hop/STI1 by migrating neuroblasts. Treatment with antibodies against Hsp90 and co-chaperone Hop/STI1, as well as Hsp90 and Hsp70 inhibitors hinder neuroblast chain migration. Time-lapse videomicroscopy analysis revealed that cell motility and average migratory speed was decreased after exposure to both antibodies and inhibitors. Antibodies recognizing Hsp90, Hsp70, and Hop/STI1 were found bound to the membranes of cells from primary SVZ cultures and biotinylation assays demonstrated that Hsp70 and Hop/STI1 could be found on the external leaflet of neuroblast membranes. The latter could also be detected in conditioned medium samples obtained from cultivated SVZ cells. Our results suggest that chaperones Hsp90, Hsp70, and co-chaperone Hop/STI1, components of the Hsp90 complex, regulate SVZ neuroblast migration in a concerted manner through an extracellular mechanism.

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Section: Discussionsupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Evidence of a Cell Surface Role for Hsp90 Complex Proteins Mediating Neuroblast Migration in the Subventricular Zone

Miyakoshi

Marques‐Coelho

Souza

et al. 2017

Front. Cell. Neurosci.

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“…Glycosphingolipids (GSLs) are glycolipids expressed on membranes of mammalian cells and involved in specific neuronal, metabolic and immune functions [2] where they participate in cell-cell and cell-substrate recognition [3], adhesion and migration [4], serve as receptors for bioactive molecules [5], modulators of signal transduction [6] regulators of cellular fate processes [7]. GSLs are particularly highly expressed in skin [7] and nervous system [8], constituting up to 12% of the total lipid content of neuronal membranes compared to up to only 2% in other tissues (AOCS lipid library).…”

Section: Introductionmentioning

confidence: 99%

Stimulation of adult neural stem cells with a novel glycolipid biosurfactant

2013

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Glycolipids are amphipatic molecules which are highly expressed on cell membranes in skin and brain where they mediate several key cellular processes. Neural stem cells are defined as undifferentiated, proliferative, multipotential cells with extensive self-renewal and are responsive to brain injury. Di-rhamnolipid: α-L-rhamnopyranosyl-(1-2)α-L-rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoic acid, also referred to as di-rhamnolipid BAC-3, is a glycolipid isolated from bacteria Pseudomonas aeruginosa. In the previous studies di-rhamnolipid enhanced dermal tissue healing and regeneration. The present study provides the first assessment of di-rhamnolipid, and glycolipid-biosurfactants in general, on the nervous system. Treatment of neural stem cells isolated from the lateral ventricle of adult mice and cultured in defined media containing growth factors at 0.5 and 1 μg/ml of di-rhamnolipid increased the number of neurospheres (2.7 and 2.8 fold, respectively) compared to controls and this effect remained even after passaging in the absence of di-rhamnolipid. Additionally, neural stem cells treated with di-rhamnolipid at 50 and 100 μg/ml in defined media supplemented with fetal calf serum and without growth factors exhibited increased cell viability indicating an interaction between di-rhamnolipid and serum components in the regulation of neural stem cells and neuroprogenitors. Intracerebroventricular administration of di-rhamnolipid at 300 and 120 ng/day increased the number of neurospheres (1.3 and 1.63 fold, respectively) that could be derived from the anterior lateral ventricles of adult mice. These results indicate that di-rhamnolipid stimulates proliferation of neural stem cells and increases their endogenous pools which may have therapeutic potential in managing neurodegenerative or neuropsychiatric disorders and promoting nervous tissue regeneration following injury.

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Expression of ganglioside 9‐O acetyl GD3 in undifferentiated embryonic stem cells

Azevedo-Pereira

Morrot

Machado

et al. 2014

Cell Biology International

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Embryonic stem cells (ES cells) express a transient and heterogeneous pattern of molecules, which suggests a notable mechanism to control self-renewal avoid the differentiation into germ layers. We show that 9-O-acetyl GD3 (9OacGD3), a highly expressed b-series ganglioside in neural stem (NS) cells, is expressed in undifferentiated mouse ES cells in a heterogeneous fashion. After sorting, undifferentiated 9OacGD3(+) ES cell population had higher levels of nestin and Sox2 mRNA than the 9OacGD3(-) cells. Even with elevated expression of these neural transcription factors, 9OacGD3(+) cells did not give rise to more neural progenitors than 9OacGD3(-) cells. Expression of 9OacGD3 was recovered from 9OacGD3(-) cell population, demonstrating that expression of this ganglioside in mouse embryonic stem cells is transient, and does not reflect cell fate. Our findings show that the ganglioside 9OacGD3 is expressed heterogeneously and transiently in ES cells, and this expression corresponds to higher levels of Sox2 and Nestin transcripts.

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Role of the 9-O-acetyl GD3 in subventricular zone neuroblast migration

Cited by 18 publications

References 47 publications

Evidence of a Cell Surface Role for Hsp90 Complex Proteins Mediating Neuroblast Migration in the Subventricular Zone

Evidence of a Cell Surface Role for Hsp90 Complex Proteins Mediating Neuroblast Migration in the Subventricular Zone

Stimulation of adult neural stem cells with a novel glycolipid biosurfactant

Expression of ganglioside 9‐O acetyl GD3 in undifferentiated embryonic stem cells

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