Role of surfactant proteins and phospholipids as markers of BOS

D’Ovidio, Frank; Ridsdale, Ross; Mura, Marco; Takahashi, Hidekazu; Gutiérrez, C.; Hutcheon, M.; Singer, L.G.; Pierre, Andrew; Hadjiliadis, Denis; Chaparro, Cecilia; Waddell, Thomas K.; Liu, M.; Post, Martin; Keshavjee, S.

doi:10.1016/j.healun.2004.11.082

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“…Reduced levels of SP-A and SP-D proteins in the bronchoalveolar lavage collected at surveillance bronchoscopies in lung transplant recipients are associated with the development of lung allograft dysfunction. 38…”

Section: Methylprednisolone Treatedmentioning

confidence: 99%

Surfactant protein A and D polymorphisms and methylprednisolone pharmacogenetics in donor lungs

Aramini

Geraghty

Lederer

et al. 2019

The Journal of Thoracic and Cardiovascular Surgery

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Objective: Surfactant proteins A and D are important molecules involved in lung allograft innate immunity. Genetic polymorphisms of surfactant proteins A and D are associated with various lung diseases. In this study, surfactant protein A and D expression responses were investigated during pharmacogenetics upon methylprednisolone treatment as observed during lung transplantation.Methods: A human cell line (NCI-H441) and precision-cut lung slices from 16 human donors were incubated with methylprednisolone, and surfactant protein A1, surfactant protein A2, and surfactant protein D messenger RNA and surfactant protein A protein expression were assayed. Surfactant protein A1, A2, and D polymorphisms and surfactant protein A gene and protein expressions were determined.Results: In NCI-H441 cells, methylprednisolone treatment at 10 À5 M and 10 À6 M reduced surfactant protein A1 and surfactant protein A2 messenger RNA and surfactant protein A protein expression (P<.05). A pharmacogenetic relationship was observed in human donor precision-cut lung slices between the surfactant protein A2 (1A x ) variants: Surfactant protein A1, A2, and D messenger RNA expression were greater for 1A 0 versus 1A 1 (P <.05); surfactant protein A1/surfactant protein A2 genotype 6A 2 6A 2 /1A 0 1A 0 (n ¼ 5) showed greater surfactant protein A1, A2, and D messenger RNA expression and surfactant protein A protein expression compared with the other surfactant protein A1/surfactant protein A2 genotypes (n ¼ 11) (P <.05).From the

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Section: Methylprednisolone Treatedmentioning

confidence: 99%