Role of redox metabolism for adaptation of aquatic animals to drastic changes in oxygen availability

Welker, Alexis F.; Moreira, Daniel C.; Campos, Élida G.; Hermes‐Lima, Marcelo

doi:10.1016/j.cbpa.2013.04.003

Cited by 165 publications

(119 citation statements)

References 246 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Journal of Experimental Biology (2014) (Hermes-Lima and Zenteno-Savín, 2002;Welker et al, 2013). Here, we found a clear response to (or preparation for) oxidative damage in G. demissa as a consequence of prolonged aerial exposure.…”

Section: Research Articlesupporting

confidence: 49%

Changes in protein expression in the salt marsh musselGeukensia demissa: evidence for a shift from anaerobic to aerobic metabolism during prolonged aerial exposure

Fields

Eurich

Gao

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

During aerial exposure (emersion), most sessile intertidal invertebrates experience cellular stress caused by hypoxia, and the amount and types of hypoxia-induced stress will differ as exposure time increases, likely leading to altered metabolic responses. We examined proteomic responses to increasing emersion times and decreasing recovery (immersion) times in the mussel Geukensia demissa, which occurs in salt marshes along the east coast of North America. Individuals are found above mean tide level, and can be emersed for over 18 h during spring tides. We acclimated mussels to full immersion at 15°C for 4 weeks, and compared changes in gill protein expression between groups of mussels that were continually immersed (control), were emersed for 6 h and immersed during recovery for 18 h (6E/18R), were emersed for 12 h and recovered for 12 h (12E/12R), or were emersed for 18 h with a 6 h recovery (18E/6R). We found clear differences in protein expression patterns among the treatments. Proteins associated with anaerobic fermentation increased in abundance in 6E/18R but not in 12E/12R or 18E/6R. Increases in oxidative stress proteins were most apparent in 12E/12R, and in 18E/6R changes in cytoskeletal protein expression predominated. We conclude that G. demissa alters its strategy for coping with emersion stress over time, relying on anaerobic metabolism for short-to medium-duration exposure, but switching to an air-gaping strategy for long-term exposure, which reduces hypoxia stress but may cause structural damage to gill tissue.

show abstract

Section: Research Articlesupporting

confidence: 49%

Changes in protein expression in the salt marsh musselGeukensia demissa: evidence for a shift from anaerobic to aerobic metabolism during prolonged aerial exposure

Fields

Eurich

Gao

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…The review by Miller et al (2010) provides a large compendium of evidence that supports the hypothesis of an oxidative stress-induced acclimation to abiotic stress. Exposure to other stressors such as hypoxia results in increased ROS formation, which has a protective effect on different cell types as demonstrated by many (see, for example, review by Welker et al, 2013;Hermes-Lima et al, 2015). The increased ROS formation activates the antioxidant defense in many species, ranging from flatworms (Zelck and Von Janowsky, 2004) to plants (Guan et al, 2000;Jiang and Zhang, 2003), and this increase in antioxidants has been coined 'preparation for oxidative stress' (Welker et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Rivera‐Ingraham

Barri

Boël

et al. 2015

Journal of Experimental Biology

View full text Add to dashboard Cite

Osmoregulating decapods such as the Mediterranean green crab Carcinus aestuarii possess two groups of spatially segregated gills: anterior gills serve mainly respiratory purposes, while posterior gills contain osmoregulatory structures. The co-existence of similar tissues serving different functions allows the study of differential adaptation, in terms of free radical metabolism, upon salinity change. Crabs were immersed for 2 weeks in seawater (SW, 37 ppt), diluted SW (dSW, 10 ppt) and concentrated SW (cSW, 45 ppt). Exposure to dSW was the most challenging condition, elevating respiration rates of whole animals and free radical formation in hemolymph (assessed fluorometrically using C-H 2 DFFDA). Further analyses considered anterior and posterior gills separately, and the results showed that posterior gills are the main tissues fueling osmoregulatory-related processes because their respiration rates in dSW were 3.2-fold higher than those of anterior gills, and this was accompanied by an increase in mitochondrial density (citrate synthase activity) and increased levels of reactive oxygen species (ROS) formation (1.4-fold greater, measured through electron paramagnetic resonance). Paradoxically, these posterior gills showed undisturbed caspase 3/ 7 activity, used here as a marker for apoptosis. This may only be due to the high antioxidant protection that posterior gills benefit from [superoxide dismutase (SOD) in posterior gills was over 6 times higher than in anterior gills]. In conclusion, osmoregulating posterior gills are better adapted to dSW exposure than respiratory anterior gills because they are capable of controlling the deleterious effects of the ROS production resulting from this salinity-induced stress.

show abstract

“…Low or transient ROS produced by mitochondria during hypoxic stress can act as signal agents to initiate the metabolic adjustments that may proportionate hypoxia tolerance (Hamanaka and Chandel 2009;Martínez-Reyes and Cuezva 2014). However, excessive ROS produced by the respiratory chain as a result of energy deprivation may lead to lipid peroxidation, protein carbonylation and DNA damage (Welker et al 2013). Enhanced ROS production and energy deprivation are the two major components of the pathogenic mechanism that are closely related to each other.…”

Section: Introductionmentioning

confidence: 98%

Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress

Zeng

Wang

et al. 2016

Fish Physiol Biochem

View full text Add to dashboard Cite

The aim of the present study was to evaluate the effect of β-glucan on acute hypoxia-induced oxidative stress and the changes in energy metabolism by determining ROS production, activities and mRNA levels of energy metabolism enzyme (PK, F-ATPase, SDH and MDH), and in gene expression of HIF-1α in the liver of large yellow croaker. Fish were injected with β-glucan at a dose of 0 or 5 mg kg(-1) body weight on 6, 4 and 2 days before exposed to 1.5 and 7.0 mg DO L(-1) for 48 h. The results showed that β-glucan enhanced survival rate and reduced ROS during the lethal hypoxic stress, indicating that β-glucan could ameliorate hypoxia-induced oxidative stress. Obtained results also showed that β-glucan could up-regulate activities and mRNA levels of PK, demonstrating that β-glucan increased anaerobic glycolysis capacity. Furthermore, a coordinated transcriptional regulation of energy metabolism enzyme genes was observed, suggesting that HIF-1α is required for regulating these genes. In conclusion, β-glucan could alleviate cute hypoxia-induced oxidative stress in large yellow croker by enhancing anaerobic glycolysis capacity, emphasizing a central role of transcription factor HIF-1α in the process.

show abstract

Role of redox metabolism for adaptation of aquatic animals to drastic changes in oxygen availability

Cited by 165 publications

References 246 publications

Changes in protein expression in the salt marsh musselGeukensia demissa: evidence for a shift from anaerobic to aerobic metabolism during prolonged aerial exposure

Changes in protein expression in the salt marsh musselGeukensia demissa: evidence for a shift from anaerobic to aerobic metabolism during prolonged aerial exposure

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress

Contact Info

Product

Resources

About