Role of Receptor for Hyaluronic Acid-mediated Motility (RHAMM) in Low Molecular Weight Hyaluronan (LMWHA)-mediated Fibrosarcoma Cell Adhesion

Kouvidi, Katerina; Berdiaki, Aikaterini; Nikitovic, Dragana; Katonis, Pavlos; Afratis, Nikolaos A.; Hascall, Vincent C.; Karamanos, Nikos K.; Tzanakakis, George N.

doi:10.1074/jbc.m111.275875

Cited by 117 publications

(94 citation statements)

References 78 publications

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“…[46–50] Many studies of cell adhesion allow cells to attach to the substrate in the absence of serum, in part because serum proteins can adsorb to the substrate and block or interfere with the matrix-integrin interactions. Because we are using monolayers that are effective at preventing the non-specific adsorption of protein, we allowed cells to attach in the presence of serum.…”

Section: Resultsmentioning

confidence: 99%

A gene expression-based comparison of cell adhesion to extracellular matrix and RGD-terminated monolayers

Sobers¹,

Wood²,

Mrksich³

2015

Biomaterials

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This work uses global gene expression analysis to compare the extent to which model substrates presenting peptide adhesion motifs mimic the use of conventional extracellular matrix protein coated substrates for cell culture. We compared the transcriptional activities of genes in cells that were cultured on matrix-coated substrates with those cultured on self-assembled monolayers presenting either a linear or cyclic RGD peptide. Cells adherent to cyclic RGD were most similar to those cultured on native ECM, while cells cultured on monolayers presenting the linear RGD peptide had transcriptional activities that were more similar to cells cultured on the uncoated substrates. This study suggests that biomaterials presenting the cyclic RGD peptide are substantially better mimics of extracellular matrix than are uncoated materials or materials presenting the common linear RGD peptide.

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Section: Resultsmentioning

confidence: 99%

A gene expression-based comparison of cell adhesion to extracellular matrix and RGD-terminated monolayers

Sobers¹,

Wood²,

Mrksich³

2015

Biomaterials

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“…Among the receptors involved in HA signaling, the CD44 is present in almost all human cells and activates signaling cascades that includes PI3K/PDK1/AKT pathway as well as Ras phosphorylation cascade involving RAF1, MEK and ERK1/2 (50, 51, 52). RHAMM receptor was described in tumor cell line (53) and in several cell types, including endothelial cells (54), and reported to trigger several cellular signaling including Ras, focal adhesion kinase (FAK), ERK1/2, protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K) (55-56). …”

Section: Discussionmentioning

confidence: 99%

Hyaluronan based hydrogels provide an improved model to study megakaryocyte–matrix interactions

Currao

Malara

Buduo

et al. 2016

Experimental Cell Research

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Hyaluronan (HA) is a glycosamminoglican involved in cell biology as well as a relevant polymer for tissue engineering and regenerative medicine. Megakaryocytes (Mks) are immersed in a mesh of extracellular matrix (ECM) components that regulate their maturation in the bone marrow (BM) and the release of platelets into the bloodstream. While fibrous ECMs such as collagens and fibronectin have been demonstrated to differently regulate Mk function and platelet release, the role of HA, that fills the majority of the BM extracellular interstitial space, has not been investigated so far. Here we demonstrated that, although human Mks express HA receptors, they are not affected by HA in terms of in vitro differentiation, maturation and platelet formation. Importantly, chemical properties of HA were exploited to generate hydrogels with entrapped ECMs that represent a useful model to more closely mimic the tridimensional characteristics of the BM environment for studying Mk function. In conclusion, in this work we demonstrated that HA is an ideal candidate for a 3D ex vivo model of human BM ECM component environment.

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“…As noted in the Introduction, many reports in various cell types and animal models have documented different biological effects of HA based on its size. In addition to HARE, the HA receptors CD44 and RHAAM also signal in response to smaller but not larger HA (62,63). Small HA fragments are thought to occur at inflammation sites and be active in inducing expression of inflammatory genes, such as TNF-␣, IL-1␤ (64).…”

Section: Discussionmentioning

confidence: 99%

The Hyaluronan Receptor for Endocytosis (HARE) Activates NF-κB-mediated Gene Expression in Response to 40–400-kDa, but Not Smaller or Larger, Hyaluronans

Pandey

Baggenstoss

Washburn

et al. 2013

Journal of Biological Chemistry

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Background: HARE mediates systemic clearance of hyaluronan (HA), which turns over continuously in tissues. Results: HARE uptake of 40 -400-kDa, but not larger or smaller, HA stimulated NF-B activation. Conclusion: HA-HARE signal complexes activate NF-B and gene transcription only with optimally sized HA. Significance: HARE responsiveness to a narrow size range of HA degradation products may be a sensing system to detect tissue ECM stress.

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Role of Receptor for Hyaluronic Acid-mediated Motility (RHAMM) in Low Molecular Weight Hyaluronan (LMWHA)-mediated Fibrosarcoma Cell Adhesion

Cited by 117 publications

References 78 publications

A gene expression-based comparison of cell adhesion to extracellular matrix and RGD-terminated monolayers

A gene expression-based comparison of cell adhesion to extracellular matrix and RGD-terminated monolayers

Hyaluronan based hydrogels provide an improved model to study megakaryocyte–matrix interactions

The Hyaluronan Receptor for Endocytosis (HARE) Activates NF-κB-mediated Gene Expression in Response to 40–400-kDa, but Not Smaller or Larger, Hyaluronans

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