Role of Phosphodiesterase Type 3A in Rat Oocyte Maturation1

Richard, François J.; Tsafriri, A.; Conti, Marco

doi:10.1095/biolreprod65.5.1444

Cited by 144 publications

(113 citation statements)

References 57 publications

(91 reference statements)

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“…However, the CDC2A activity was lower in adult than in prepubertal mouse oocytes. Activities of PDE3 in rat oocytes increased significantly in vitro prior to meiotic resumption, but returned to the basal level at the peak of GVBD (Richard et al 2001). In this study, while cAMP levels declined significantly prior to GVBD in both prepubertal and adult oocytes, the cAMP level of adult was still higher than that of prepubertal oocytes.…”

contrasting

confidence: 41%

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Han¹,

Cao²,

Wang³

et al. 2010

Reproduction

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Although studies suggest that the low competence of oocytes from prepubertal animals is due to their insufficient cytoplasmic maturation and that FSH improves oocyte maturation possibly by retarding meiotic progression and allowing more time for cytoplasmic maturation, the mechanisms by which puberty and gonadotropins regulate meiotic progression require additional detailed studies. For the first time, we observed that while meiotic progression was significantly slower, the maturation-promoting factor (MPF) activity of oocytes was significantly higher in prepubertal than in adult mice. To resolve this contradiction, we specified the molecules regulating the MPF activity and their localization during oocyte maturation in prepubertal and adult mice primed with or without gonadotropins. Our tests using corresponding enzyme regulators suggested that while activities of protein kinase A were unaffected, the activity of adenylate cyclase (ADCY) and phosphodiesterase increased while cell division cycle 2 homolog A (CDC2A) decreased significantly after puberty. While most of the adult oocytes had CDC2A protein concentrated in the germinal vesicle (GV) region, the majority of prepubertal oocytes showed no nuclear concentration of CDC2A. Maximally priming mice with equine chorionic gonadotropin brought the above parameters of prepubertal oocytes close to those in adult oocytes. Together, the results suggest that puberty and gonadotropin control oocyte meiotic progression mainly by regulating the ADCY activity and the concentration of the activated MPF toward the GV region.

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contrasting

confidence: 41%

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Han¹,

Cao²,

Wang³

et al. 2010

Reproduction

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“…In that system, treatment with the uncoupler 1-heptanol induced abrupt chromatin condensation and decreased in transcription. However, the addition of the oocyte-specific PDE3 inhibitor cilostamide [31][32][33] to the culture medium nullified this effect indicating that the functional status of GJC may affect both transcriptional activity and remodeling of largescale chromatin configuration in growing oocytes, potentially through cAMP-dependent mechanism(s) [19].…”

Section: Discussionmentioning

confidence: 99%

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Lodde

Franciosi

Tessaro

et al. 2013

J Assist Reprod Genet

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Purpose This study was aimed to test the hypothesis that gap junction mediated communications (GJC) are required to allow the progressive chromatin configuration remodeling (from GV1 to GV3) process to occur in fully grown oocytes in order to gain the final step of developmental competence acquisition, and that a premature disruption of GJC can alter this process. Methods Bovine cumulus-oocytes complexes collected from medium antral follicles were cultured for 2, 4, 6 and 8 h in the presence of 10 −4 IU/ml of r-hFSH and with 2 mM of the nonselective PDE inhibitor 3-isobutyl-1-methyl-xanthine (IBMX) to prevent meiotic resumption. GJC functionality and chromatin configuration were monitored during the culture period. After meiotic arrest, the developmental capability of oocytes was assessed after IVM and IVF. Results IBMX was effective in significantly sustaining GJC up to 6 h and maintaining meiotic arrest, when compared to control group. Moreover, the percentage of oocytes with less condensed chromatin (GV1) decreased within 4 h of culture, while the proportion of GV2 oocytes gradually increased up to 6 h.Interestingly, a decline in the proportion of GV2 oocytes and an increase in the proportion of GV3 oocytes were observed after 6 h of culture, when the major drop of GJC occurred. On the contrary, when GJC were uncoupled by adding 3 mM of 1-heptanol or through cumulus cells removal, chromatin condensation occurred rapidly throughout the culture period, more promptly in denuded oocytes. Moreover, the maintenance of GJC during meiotic arrest was accompanied by a significant increase of developmental competence compared to the control, as indicated by a higher percentage of hatched blastocysts and blastocyst cell number. Conclusions Altogether, our data indicate that both paracrine and junctional mechanisms are involved in modulating largescale chromatin structure during the final phase of oocyte differentiation.

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“…Finally, it is necessary to maintain cAMP concentrations within the oocyte during sample collection and processing before the assay. This is often accomplished by including IBMX in the collection medium, since the majority of PDE activity in mammalian oocytes is due to PDE3, which is sensitive to IBMX (Downs et al 1989;Conti et al 1998;Richard et al 2001;Jensen et al 2002;Thomas et al 2002;Liang et al 2005;Nogueira et al 2006;Sasseville et al 2009). Although IBMX was capable of inhibiting enough PDE activity to maintain meiotic arrest in feline oocytes, it is possible that other IBMX-insensitive PDEs are present and active in feline oocytes and capable of degrading some cAMP before analysis.…”

Section: Reversible Meiotic Arrest In Feline Oocytesmentioning

confidence: 99%

“…Both of these chemicals have been used extensively in multiple species with consistent, predicted effects (Bilodeau et al 1993;Fan et al 2002;Thomas et al 2002;Chen et al 2009;Downs 2011). In mice, rats, pigs, cattle, macaques and humans, the majority of PDE activity within the oocyte is due to PDE3, which is sensitive to IBMX (Downs et al 1989;Conti et al 1998;Richard et al 2001;Jensen et al 2002;Thomas et al 2002;Liang et al 2005;Nogueira et al 2006;Sasseville et al 2009). By inhibiting PDE3 activity, IBMX maintains meiotic arrest by preventing a decline in cAMP concentrations within the oocyte after recovery (Schultz et al 1983;Luciano et al 2004;Albuz et al 2010) or permitting the accumulation of cAMP produced by intra-oocyte adenylate cyclase(s) (Mehlmann 2005).…”

Section: Introductionmentioning

confidence: 99%

Reversible meiotic arrest in feline oocytes

Herrick

2014

Reprod. Fertil. Dev.

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Abstract. Increasing intracellular concentrations of cyclic adenosine monophosphate (cAMP) within the cumulusoocyte complex (COC) inhibits or delays spontaneous oocyte maturation and improves the developmental competence of the oocyte in many species, but information for carnivores is limited. The objectives of the present study were to describe the effects of isobutyl methylxanthine (IBMX), which decreases cAMP degradation, and forskolin, which increases cAMP production, on spontaneous and induced maturation (by equine chorionic gonadotrophin (eCG) and epidermal growth factor (EGF)) of feline oocytes and to evaluate the reversibility of IBMX-induced arrest by measuring the resumption of meiosis and embryonic development following IVF. IBMX decreased (P , 0.05) the incidence of spontaneous (6.7% vs 42.0%, metaphase II (MII)) and induced (5.6% vs 66.1% MII) maturation after 24 h of culture. In contrast, forskolin stimulated meiosis (81.7% MII; P , 0.05). Following 12 h of culture with IBMX and an additional 24 h with eCG and EGF in the absence of IBMX, the proportions of oocytes reaching MII (66.1%), cleaving (79.9%) and developing to the blastocyst stage (15.3%) were similar (P . 0.05) to oocytes cultured continuously with eCG and EGF (70.2%, 83.0% and 18.1%, respectively). These results demonstrate that IBMX reversibly inhibits both spontaneous and eCGþEGF-induced meiosis in feline oocytes without compromising the oocyte's developmental competence.

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Role of Phosphodiesterase Type 3A in Rat Oocyte Maturation1

Cited by 144 publications

References 57 publications

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Reversible meiotic arrest in feline oocytes

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