Role of p53 codon 72 polymorphism in chromosomal aberrations and mitotic index in patients with chronic hepatitis B

Akbas, Halit; Yalçın, Kendal; Isi, Hilmi; Tekeş, Selahattin; Atay, Ahmet Engin; Akkuş, Zeki; Budak, Turgay

doi:10.1590/s0100-879x2012007500129

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…However, isolated anti-HBc refers to the presence of anti-HBc in the serum without HBsAg or HBsAb and that is because of the resolved HBV infection, due to that HBsAb had declined to the undetectable level. HBV testing during the window period or chronic infection, HBsAg cannot be detected due to protein mutation which makes it undetectable by certain diagnostic assays (Akbaş et al, 2012). In this study, our results indicated that the HBV serological markers, such as HBeAg, HBcAg and Anti-HBe were significantly decreased in HCC and cirrhosis groups compared to chronic group (P = 0.022, P = 0.001 and P = 0.041, respectively).…”

Section: Controlmentioning

confidence: 59%

Measurement of the Gene Expression and Polymorphisms of c-myc and p53 Genes in HBV Infected Patients

Koutb¹,

Abdel-Rahman²,

Hassouna³

et al. 2021

OnLine Journal of Biological Sciences

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This study explained the c-myc and p53 gene expression and polymorphisms in chronic (40), cirrhosis (30) and Hepatocellular Carcinoma, (HCC) (30) patients related to Hepatitis B Virus (HBV) infection and healthy control (50) in Egypt. Where, c-myc (intron 8) and p53 (codon 72) gene expression and polymorphisms were determined using qRT-PCR and PCR-RFLP techniques. The results showed that c-myc gene expression (2 ddct) was significantly increased in chronic (1.38), cirrhosis (1.47) and HCC (5.59) compared to the control group (1.00), while p53 gene expression (2 ddct) was significantly decreased compared to the control group (0.82, 0.65, 0.33 and 1.00, respectively). In HCC group, c-myc genotype (CC) was predominant (90%) more than cirrhosis, chronic and control (73.33, 22.5 and 6%, respectively), GG genotype was predominant in control (70%) more than chronic, cirrhosis and HCC groups (67.5, 6.66 and 6.66%, respectively) and GC genotype was high in control (24%) more than cirrhosis, chronic and HCC groups (20, 10 and 3.33%, respectively). p53 PP (88%) genotype was predominant in control more than chronic, cirrhosis and HCC groups (30, 30 and 6.66%, respectively), AA genotype was predominant in HCC group (73.33%) more than chronic, cirrhosis and control (50, 10 and 4%, respectively) and genotype PA was predominant in cirrhosis (60%) more than chronic, HCC and control (20, 20 and 8%, respectively). These results suggest clearly that both c-myc and p53 gene expression and polymorphisms influence clinical outcome and progression of HBV infection and then considered an accurate genetic biomarker to determine and predict the progression of HBV infection.

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Section: Controlmentioning

confidence: 59%

Measurement of the Gene Expression and Polymorphisms of c-myc and p53 Genes in HBV Infected Patients

Koutb¹,

Abdel-Rahman²,

Hassouna³

et al. 2021

OnLine Journal of Biological Sciences

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“…(10,17) Knowing the diagnostic o the patients, infected with HCV, medical specialists could aplay the proper treatment. (14,15) HCV infection affect both genders, male and female in specially adults. (3,9)There are known patients diagnosed with HCV, also with comorbidities.…”

Section: Introductionmentioning

confidence: 99%

Laboratory Methods in the Screening of Viral C Hepatitis

Cheşcă¹,

Medetova²,

Abdulina³

et al. 2022

Preprint

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Objective: The study aimed to manage and to analyse the results of the laboratory tests, available nowadays, used from routine clinical practice, for screening of hepatitis C. Methods: comparison of ELISA method results (Enzyme-Linked Immunosorbent Assay) and chemiluminescence methods results. Beside previously mentioned, the study show the structural comparison of normal liver and pathologic liver with hepatic cirrhosis, using permanent samples colored after the technique protocol. Statistical analysis of this study results, was performed using the laboratory informatic system. Results: The results of the study are substantial and intricate. For this purpose, the results of preliminary EСL screening method of patients at risk for HCV who took part in the study, are presented in tables and figures. Results of this study are various and are correlate from different perspectives. Also good to mention that the correlations of results were used in order to identify a possible relationships between indicators of ELISA method and ECL index. More than, correlations antibodies detected in ECL and ELISA are point out. Conclusion: EСL and ELISA method results, are relevant for screening and for diagnostic confirmation in HCV risk patients. Unfotunately in the present study, were impossible to conclude about false-negative results. Good to know our opinion that RT-PCR technique, it is considered proper for the diagnosis of HCV.

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Is hemodialysis a reason for unresponsiveness to hepatitis B vaccine? Hepatitis B virus and dialysis therapy

Şit¹

2015

WJH

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Impaired renal function is associated with a high risk of chronicity of hepatitis B virus (HBV) infection. Patients on hemodialysis (HD) or peritoneal dialysis are at an increased risk of viral transmission due to frequent necessity of blood product transfer as well as use of contaminated dialysate or dialysis materials. Additionally, health professionals may cause viral spread via contaminated hands and carelessness against hygiene rules. The frequency of chronic HBV infection may be as high as 80% in patients on renal replacement therapies. This is because HBV vaccination is essential to eliminate chronic HBV infection. However, response rates of HD patients to HBV vaccination vary between 10%-50%. Dialysis adequacy and early vaccination before the onset of dialysis therapy seem to be major determinants of high seroconversion rates. Older age, male gender, duration of dialysis therapy and nutritional status are other well-known factors associated with seroconversion rate. There are controversial reports regarding the role of the presence of diabetes mellitus, HCV positivity, erythropoietin resistance, hyperparathyroidism, and vitamin D inadequacy. The role of genetic alteration in the functions or production of cytokines still needs to be elucidated.

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Role of p53 codon 72 polymorphism in chromosomal aberrations and mitotic index in patients with chronic hepatitis B

Cited by 3 publications

References 33 publications

Measurement of the Gene Expression and Polymorphisms of c-myc and p53 Genes in HBV Infected Patients

Measurement of the Gene Expression and Polymorphisms of c-myc and p53 Genes in HBV Infected Patients

Laboratory Methods in the Screening of Viral C Hepatitis

Is hemodialysis a reason for unresponsiveness to hepatitis B vaccine? Hepatitis B virus and dialysis therapy

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