Role of nitric oxide in alcohol-induced changes in lipid profile of moderate and heavy alcoholics

Kavitha, G; Reddy, Vaddi Damodara; Maturu, Paramahamsa; Akhtar, Perveen; Nallanchakravarthula, Varadacharyulu

doi:10.1016/j.alcohol.2007.10.006

Cited by 11 publications

(4 citation statements)

References 55 publications

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“…A decrease in membrane uidity observed in alcoholics in the present study may be an adaptive biochemical change in alcoholics to counteract the uidizing effect of alcohol. This study also strengthens and con rms the earlier reports [27] where in the role of NO in alcohol induced changes in lipid pro le of alcoholics was being demonstrated.…”

Section: Discussionsupporting

confidence: 92%

Biochemical and biophysical changes in plasma and erythrocyte membranes of alcohol consuming type 2 diabetics: A clinical study

Naresh

Hebbani²,

Reddy³

et al. 2021

Preprint

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Background and aims: Effects of alcohol consumption on blood glucose levels is unpredictable and more so with a known type 2 diabetic. Since type 2 diabetes is a chronic condition with impairment of glucose metabolism, influence of excess alcohol consumption in such a derailed metabolism is ought to be investigated. Our aim was to understand the interpolating relationship between the metabolisms of glucose and alcohol, by investigating the biochemical and biophysical changes in plasma and erythrocytes respectively. Methods We performed a clinical study with 20 human subjects wherein non-alcoholics, non-diabetics were considered as controls and the test subjects were categorized as alcoholics, diabetics and alcoholic diabetics. Findings were analysed against the control group. Results Increased plasma AST, ALT, ALP, and LDH enzyme activity; higher levels of nitric oxide, thiobarbituric acid reactive species (TBARS) both in plasma and erythrocyte lysate; higher fasting and postprandial glucose, glycated haemoglobin levels (Hb1Ac) levels; elevated levels of erythrocyte membrane total cholesterol / phospholipids (C/P) ratio and altered erythrocyte membrane fluidity in the alcoholic diabetics was noted. Conclusion Alcohol induced oxidative and nitrosative stress during its metabolism and its worsening effects in type II diabetics leading to a failure in the overall metabolic homeostasis is evident from the study.

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Section: Discussionsupporting

confidence: 92%

Biochemical and biophysical changes in plasma and erythrocyte membranes of alcohol consuming type 2 diabetics: A clinical study

Naresh

Hebbani²,

Reddy³

et al. 2021

Preprint

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“…Lipids extraction and total cholesterol and phospholipids were determined as described previously. ( 29 ) To the lysed membrane preparations, methanol and chloroform was added in 1:2 ratio. After 30 min the filtrate was collected from the mixture, and the residue was used again for another extraction.…”

Section: Methodsmentioning

confidence: 99%

Association between alcohol-induced erythrocyte membrane alterations and hemolysis in chronic alcoholics

Bulle

Reddy

Padmavathi

et al. 2017

J. Clin. Biochem. Nutr.

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The present study aimed to understand the association between erythrocyte membrane alterations and hemolysis in chronic alcoholics. Study was conducted on human male volunteers aged between 35–45 years with a drinking history of 8–10 years. Results showed that plasma marker enzymes AST, ALT, ALP and γGT were increased in alcoholic subjects. Plasma and erythrocyte membrane lipid peroxidation, erythrocyte lysate nitric oxide (NOx) levels were also increased significantly in alcoholics. Furthermore, erythrocyte membrane protein carbonyls, total cholesterol, phospholipid and cholesterol/phospholipid (C/P) ratio were increased in alcoholics. SDS-PAGE analysis of erythrocyte membrane proteins revealed that increased density of band 3, protein 4.2, 4.9, actin and glycophorins, whereas glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glycophorin A showed slight increase, however, decreased ankyrin with no change in spectrins (α and β) and protein 4.1 densities were observed in alcoholics. Moreover, alcoholics red blood cells showed altered morphology with decreased resistance to osmotic hemolysis. Increased hemolysis showed strong positive association with lipid peroxidation (r = 0.703, p<0.05), protein carbonyls (r = 0.754, p<0.05), lysate NOx (r = 0.654, p<0.05) and weak association with C/P ratio (r = 0.240, p<0.05). Bottom line, increased lipid and protein oxidation, altered membrane C/P ratio and membrane cytoskeletal protein profile might be responsible for the increased hemolysis in alcoholics.

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“…Moderate alcohol consumers had higher plasma levels of nitrite and nitrate than teetotalers with a more favourable lipid profile. NO in plasma has been involved in the cardioprotective effect but was considered to have a biphasic role and a dose-dependent effect regarding the findings of even highest NO levels and higher lipid-related cardiovascular risk in heavy drinkers ( 37 ) . The moderate intake of red wine (100 ml daily for 3 weeks) enhanced circulating endothelial progenitor cell and plasma levels of NO in healthy subjects while beer (250 ml) and vodka (30 ml) consumption did not produce any effect ( 38 ) .…”

Section: Moderate Alcohol Consumption Inflammation and Cvdmentioning

confidence: 99%

Potential health benefits of moderate alcohol consumption: current perspectives in research

et al. 2012

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The benefits of moderate amounts of alcohol for a better health and longer life expectancy compared with abstinence have been suggested by the findings of numerous studies. However, controversies have emerged regarding the influence of confounding factors and the systematic errors that might have been inadvertently disregarded in the early studies. This review includes a description of the findings of those research studies published in the last 5 years on the effects of moderate alcohol consumption on all-cause mortality, CVD and inflammation, the immune system, insulin sensitivity, non-alcoholic fatty liver disease (NAFLD) and cancer. Promising evidences exist from both animal studies and human clinical trials regarding intermediate end-points of CHD and insulin sensitivity, such as HDL, adiponectin or fibrinogen. However, controversies and inconsistent findings exist regarding many of these diseases and related functions and biomarkers. Further research and human randomised-controlled trials with adequate standardisation of the study conditions are necessary in order to draw a comparison between studies, establish the causal effect of moderate alcohol intake on disease protection and reach consensus on the circumstances that allow the recommendation of moderate alcohol habitual intakes as a strategy for health maintenance.

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Role of nitric oxide in alcohol-induced changes in lipid profile of moderate and heavy alcoholics

Cited by 11 publications

References 55 publications

Biochemical and biophysical changes in plasma and erythrocyte membranes of alcohol consuming type 2 diabetics: A clinical study

Biochemical and biophysical changes in plasma and erythrocyte membranes of alcohol consuming type 2 diabetics: A clinical study

Association between alcohol-induced erythrocyte membrane alterations and hemolysis in chronic alcoholics

Potential health benefits of moderate alcohol consumption: current perspectives in research

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