2013
DOI: 10.1021/bi400324c
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Abstract: Cytochrome c undergoes structural variations during the apoptotic process; such changes have been related to modifications occurring in the protein when it forms a complex with cardiolipin, one of the phospholipids constituting the mitochondrial membrane. Although several studies have been performed to identify the site(s) of the protein involved in the cytochrome c−cardiolipin interaction, to date the location of this hosting region(s) remains unidentified and is a matter of debate. To gain deeper insight int… Show more

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Cited by 85 publications
(144 citation statements)
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“…The Arg mutation does not affect the protein, as the spectrum is identical to that of wt cyt c, while a slight decrease of the CT band at 695 is observed in the Ala mutant. Although significant changes have been observed previously for the Asn mutants [28], the CD properties of the Lys72Arg and Lys73Arg mutants are similar to those of the wt protein. The far-UV dichroic spectra (not shown) are typical of proteins possessing α-helix secondary structure, and the ellipticity is comparable to that of the native form.…”
Section: Structural Properties and Stability Of The Lys72arg And Lys7mentioning
confidence: 38%
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“…The Arg mutation does not affect the protein, as the spectrum is identical to that of wt cyt c, while a slight decrease of the CT band at 695 is observed in the Ala mutant. Although significant changes have been observed previously for the Asn mutants [28], the CD properties of the Lys72Arg and Lys73Arg mutants are similar to those of the wt protein. The far-UV dichroic spectra (not shown) are typical of proteins possessing α-helix secondary structure, and the ellipticity is comparable to that of the native form.…”
Section: Structural Properties and Stability Of The Lys72arg And Lys7mentioning
confidence: 38%
“…Due to their sensitivity to laser irradiation, all the mutants were cooled by a gentle flow of N 2 gas passed through liquid N 2 , to minimize local heating of the protein by the laser beam, and the sample was changed every 15 min. A relatively low laser power of 2 mW was used for the Arg and Ala mutants, compared to 6 mW used previously for the Asn mutants [28].…”
Section: Resonance Raman Measurementsmentioning
confidence: 99%
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“…Most studies of this kind have used the exterior surface of liposome membranes to follow the interaction of cytochrome c with cardiolipin. An impressive number have been conducted on the mechanism of this unfolding phase (4,29,45,47,48) and this gross structural perturbation leads to the activation of the peroxidase activity that subsequently initiates critical modifications of cardiolipin (26,28,29,49,50).…”
Section: Discussionmentioning
confidence: 99%