Role of long noncoding RNA-mediated competing endogenous RNA regulatory network in hepatocellular carcinoma

Niu, Zhaojian; Wang, Wenhong; Dong, Xiaofeng; Tian, Li-Mei-Li

doi:10.3748/wjg.v26.i29.4240

Cited by 46 publications

(35 citation statements)

References 169 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 32 Growing evidence illuminated that cytoplasmic lncRNAs could operate as ceRNAs for specific miRNAs, thereby upregulating the target mRNAs at the post-transcriptional level. 33–35 Using cell cytoplasmic/nuclear fractionation assay, most NOP14-AS1 was identified to be located in the cytoplasm of TSCC cells, implying that lncRNAs may execute its roles via the ceRNA theory. By searching the ENCORI Starbase 3.0, NOP14-AS1 was found to contain theoretical binding sites of miR-665.…”

Section: Discussionmentioning

confidence: 99%

LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis

Li¹,

Fan²,

Liu³

et al. 2021

CMAR

View full text Add to dashboard Cite

Purpose The expression profile, clinical effects, and detailed roles of NOP14 antisense RNA 1 (NOP14-AS1) in tongue squamous cell carcinoma (TSCC) remain ambiguous and need to be further explored. Thus, this work was initiated to offer further solid evidence regarding the expression and roles of NOP14-AS1 in TSCC. Furthermore, additional efforts were exerted to reveal the molecular events by which NOP14-AS1 affects the malignant behaviours of TSCC. Methods NOP14-AS1 expression was detected in TSCC tissues and cell lines using quantitative reverse transcription-polymerase chain reaction. Cell Counting Kit-8 assay, flow cytometric analysis, Transwell migration and invasion assays, and xenograft tumor model analysis were performed to assess the malignant biological behaviors of TSCC cells after NOP14-AS1 depletion. Mechanistic studies were performed using bioinformatics analysis, luciferase reporter assay, RNA immunoprecipitation, and rescue experiments. Results NOP14-AS1 upregulation was identified in TSCC tissues and cell lines. Patients with TSCC exhibiting a high NOP14-AS1 expression faced shorter overall survival than those with a low NOP14-AS1 expression. Functionally, NOP14-AS1 depletion facilitated apoptosis and impeded cell proliferation, migration, and invasion in TSCC. In vivo, the growth of TSCC cells was hindered by NOP14-AS1 depletion. Mechanically, NOP14-AS1 functioned as a competing endogenous RNA by sponging microRNA-665 (miR-665), thereby overexpressing the target high mobility group box 3 (HMGB3) of miR-665. Lastly, rescue experiments confirmed that the introduction of HMGB3 overexpression plasmid or miR-665 inhibitor could abrogate the inhibition of aggressive phenotypes triggered by NOP14-AS1 knockdown. Conclusion NOP14-AS1 executed pro-oncogenic activities in TSCC cells by targeting the miR-665/HMGB3 axis. The NOP14-AS1/miR-665/HMGB pathway may be a valuable prognostic indicator and therapeutic target for preventing TSCC.

show abstract

Section: Discussionmentioning

confidence: 99%

LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis

Li¹,

Fan²,

Liu³

et al. 2021

CMAR

View full text Add to dashboard Cite

show abstract

“…8 lncRNAs that act as competing endogenous RNAs (ceRNAs) are able to sequester miRNAs (also called miRNA sponges) via competing with endogenous mRNAs to bind to the miRNA response elements (MREs) in a sequence-specific manner, which results in the reduction of combination between miRNAs and their target mRNAs, and thus weaken the suppressive function of miRNAs on the downstream target gene expressions. 9,10 To date, increasing evidence has identified the carcinogenic or tumor suppressive role of lncRNA-mediated ceRNA networks involved in NSCLC occurrence and dominating crucial cancer hallmark processes like proliferation, apoptosis, angiogenesis, immune escape, drug resistance, as well as epithelial-mesenchymal transition (EMT) and metastasis across NSCLC progression, [11][12][13] accordingly, clarifying the molecular mechanism of the complex "ceRNA-lncRNA-miRNA-mRNA" networks underlying tumor biology will provide novel insight into the potential diagnostic and therapeutic biomarkers of NSCLC (Figure 1). lncRNA prostate androgen regulated transcript 1 (PART1) is located on chromosome 5q12 and is considered to be modulated by androgens and predominantly expressed in the prostate gland.…”

Section: Introductionmentioning

confidence: 99%

LncRNA PART1 promotes lung squamous cell carcinoma progression via miR-185-5p/Six1 axis

et al. 2020

View full text Add to dashboard Cite

Dysregulation of the long non-coding RNA prostate androgen regulated transcript 1 (lncRNA PART1) is involved in the tumorigenesis of various cancers. However, little is known about its function and molecular mechanism in the development of lung squamous cell carcinoma (LSCC). In this study, we examined the expression of PART1 in LSCC clinical tissue samples and cell lines, and gain- and loss-of-function experiments were performed to explore the function of PART1 in LSCC proliferation, invasion and migration. We found that PART1 was overexpressed in both LSCC tissues and cell lines. Functional studies revealed that PART1 knockdown significantly suppressed cell proliferation, invasion and migration but enhanced apoptosis in LSCC cells, whereas overexpression of PART1 showed the opposite results. Mechanistically, we identified that PART1 acted as a sponge of miR-185-5p, and sineoculis homeobox homolog 1 (Six1) was a direct downstream target of miR-185-5p. Moreover, restoration of miR-185-5p or silencing of Six1 partially abolished the oncogenic effect of PART1 in LSCC cells. Clinically, The areas under the receiver operating characteristic (ROC) curve of PART1, miR-185-5p, and Six1 were 0.7857, 0.7332, 0.8112, respectively. Notably, high PART1, low miR-185-5p, and high Six1 expressions were significantly associated with severe clinical parameters and were the independent risk factors for poor prognosis of LSCC patients. Thus, we concluded that the PART1/miR-185-5p/Six1 axis might serve as a novel biomarker for the diagnosis and treatment of LSCC.

show abstract

“…Existing evidence indicated that there are interactions among RNA molecules, such as miRNAs and mRNAs, lncRNAs and mRNAs, and lncRNAs and miRNAs; these RNA molecules collaborate to form a dynamic regulatory network acting as competitive endogenous RNAs (ceRNAs) ( Niu et al, 2020 ). In the present study, a lncRNA-miRNA-mRNA network was built based on the interactions between lncRNA-miRNA, miRNA-mRNA, and lncRNA-mRNA.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics-Based Analysis of the lncRNA-miRNA-mRNA Network and TF Regulatory Network to Explore the Regulation Mechanism in Spinal Cord Ischemia/Reperfusion Injury

Wang

Han

et al. 2021

Front. Genet.

View full text Add to dashboard Cite

BackgroundSpinal cord ischemia/reperfusion injury (SCII) is a catastrophic complication involved with cardiovascular, spine, and thoracic surgeries and can lead to paraplegia. Nevertheless, the molecular mechanism of SCII remain ill-defined.MethodsExpression profiling (GSE138966) data were obtained from GEO database. Then, differentially expressed (DE) lncRNAs and DEmRNAs were screened out with p < 0.05, and | fold change| > 1.5. Aberrant miRNAs expression in SCII was obtained from PubMed. Functional enrichment analysis of overlapping DEmRNAs between predicted mRNAs in miRDB database and DEmRNAs obtained from GSE138966 was performed using cluster Profiler R package. The lncRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) network was established in light of ceRNA theory. The key lncRNAs in the ceRNA network were identified by topological analysis. Subsequently, key lncRNAs related ceRNA-pathway network and transcription factors (TFs)-mRNAs network were constructed. Simultaneously, the expression levels of hub genes were measured via qRT-PCR.ResultsThe results in this study indicated that 76 miRNAs, 1373 lncRNAs, and 4813 mRNAs were differentially expressed in SCII. A SCII-related ceRNA network was constructed with 154 ncRNAs, 139 mRNAs, and 51 miRNAs. According topological analysis, six lncRNAs (NONRATT019236.2, NONRATT009530.2, NONRATT026999.2, TCONS_00032391, NONRATT023112.2, and NONRATT021956.2) were selected to establish the ceRNA-pathway network, and then two candidate hub lncRNAs (NONRATT009530.2 and NONRATT026999.2) were identified. Subsequently, two lncRNA-miRNA-mRNA regulatory axes were identified. NONRATT026999.2 and NONRATT009530.2 might involve SCII via miR-20b-5p/Map3k8 axis based on the complex ceRNA network. SP1 and Hnf4a acting as important TFs might regulate Map3k8. Furthermore, qRT-PCR results showed that the NONRATT009530.2, NONRATT026999.2, Map3k8, Hfn4a, and SP1 were significantly upregulated in SCII of rats, while the miR-20b-5p was downregulated.ConclusionOur results offer a new insight to understand the ceRNA regulation mechanism in SCII and identify highlighted lncRNA-miRNA-mRNA axes and two key TFs as potential targets for prevention and treatment of SCII.

show abstract

Role of long noncoding RNA-mediated competing endogenous RNA regulatory network in hepatocellular carcinoma

Cited by 46 publications

References 169 publications

LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis

LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis

LncRNA PART1 promotes lung squamous cell carcinoma progression via miR-185-5p/Six1 axis

Bioinformatics-Based Analysis of the lncRNA-miRNA-mRNA Network and TF Regulatory Network to Explore the Regulation Mechanism in Spinal Cord Ischemia/Reperfusion Injury

Contact Info

Product

Resources

About