Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

Olivares-Navarrete, René; Rodil, S.E.; Hyzy, Sharon L.; Dunn, Ginger R.; Almaguer‐Flores, Argelia; Schwartz, Zvi; Boyan, Barbara D.

doi:10.1016/j.biomaterials.2015.01.035

Cited by 93 publications

(61 citation statements)

References 68 publications

(61 reference statements)

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“…The pattern of integrin expression is similar to that reported by other authors studying the effect of the microtopography; the cells exhibited an increase in the expression of α1, α2, and β1 mRNA on the surface with the highest differentiation, while α5 showed an opposite behavior. In this work, it was observed that in all cases, the SLA surfaces (coated or uncoated) presented the lowest attachment and cell number, but the highest gene and protein expression associated with osteogenic differentiation.…”

Section: Discussionsupporting

confidence: 88%

“…In this work, it was observed that in all cases, the SLA surfaces (coated or uncoated) presented the lowest attachment and cell number, but the highest gene and protein expression associated with osteogenic differentiation. Previous studies have shown that common integrin subunits are upregulated by the surface roughness in biomaterials with different chemistries; expression of α1, α2, and β1 were higher on rough titanium than on smooth surfaces. Moreover, it has been demonstrated that integrin expression is modulated when cells are grown on Ti‐based surfaces with different roughness, and control of integrin expression is partially regulated by Wnt signaling .…”

Section: Discussionmentioning

confidence: 88%

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Enhancing the osteoblastic differentiation through nanoscale surface modifications

Silva-Bermúdez

Almaguer‐Flores

García

et al. 2016

J Biomedical Materials Res

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Human mesenchymal stem cells (MSCs) showed larger differentiation into osteoblasts on nanoscale amorphous titanium oxide (TiO ) coatings in comparison to polycrystalline TiO coatings or native oxide layers. In this article, we showed that the subtle alterations in the surface properties due to a different atomic ordering of titanium oxide layers could substantially modify the osteoblastic differentiation of MSCs. Amorphous (a) and polycrystalline (c) TiO coatings were deposited on smooth (PT) and microstructured sandblasted/acid-etched (SLA) Ti substrates using a magnetron sputtering system. The surface roughness, water contact angle, structure, and composition were measured using confocal microscopy, drop sessile drop, X-ray diffraction, and X-ray photoelectron spectroscopy, respectively. The ∼70-nm-thick coatings presented a well-passivated and uniform TiO (Ti ) surface composition, while the substrates (native oxide layer) showed the presence of Ti atoms in lower valence states. The polycrystalline TiO -coated surfaces (cPT and cSLA) showed the same cell attachment as the uncoated metallic surfaces (PT and SLA), and in both cases, it was lower on the rough than on the smooth surfaces. However, attachment and differentiation were significantly increased on the amorphous TiO -coated surfaces (aPT and aSLA). The amorphous coated Ti surfaces presented the highest expression of integrins and production of osteogenic proteins in comparison to the uncoated and crystalline-coated Ti surfaces. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 498-509, 2017.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 88%

Enhancing the osteoblastic differentiation through nanoscale surface modifications

Silva-Bermúdez

Almaguer‐Flores

García

et al. 2016

J Biomedical Materials Res

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“…41 In particular, β1 integrin interacting with FN is known to play a major role in roughness recognition on graphitic carboncoated substrates. 42 In this study, GPS was coated with FN to increase hNSC adhesion, and thus 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 hNSCs ( Figure 5B). qRT-PCR analysis for FAK and vinculin revealed that the gene expression levels of those focal adhesion proteins in hNSCs grown on the GPS with 5-µm groove width was remarkably downregulated by the treatments with β1 integrin antibodies, blebbistatin, and Y27632, compared with no treatment ( Figure 5C).…”

Section: Mechanisms Of Enhanced Differentiation Of Hnscs By Hierarchimentioning

confidence: 98%

Graphene Oxide Hierarchical Patterns for the Derivation of Electrophysiologically Functional Neuron-like Cells from Human Neural Stem Cells

Yang

Lee

et al. 2016

ACS Appl. Mater. Interfaces

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Graphene has shown great potential for biomedical engineering applications due to its electrical conductivity, mechanical strength, flexibility, and biocompatibility. Topographical cues of culture substrates or tissue-engineering scaffolds regulate the behaviors and fate of stem cells. In this study, we developed a graphene oxide (GO)-based patterned substrate (GPS) with hierarchical structures capable of generating synergistic topographical stimulation to enhance integrin clustering, focal adhesion, and neuronal differentiation in human neural stem cells (hNSCs). The hierarchical structures of the GPS were composed of microgrooves (groove size: 5, 10, and 20 μm), ridges (height: 100-200 nm), and nanoroughness surfaces (height: ∼10 nm). hNSCs grown on the GPS exhibited highly elongated, aligned neurite extension along the ridge of the GPS and focal adhesion development that was enhanced compared to that of cells grown on GO-free flat substrates and GO substrates without the hierarchical structures. In particular, GPS with a groove width of 5 μm was found to be the most effective in activating focal adhesion signaling, such as the phosphorylation of focal adhesion kinase and paxillin, thereby improving neuronal lineage commitment. More importantly, electrophysiologically functional neuron-like cells exhibiting sodium channel currents and action potentials could be derived from hNSCs differentiated on the GPS even in the absence of any of the chemical agents typically required for neurogenesis. Our study demonstrates that GPS could be an effective culture platform for the generation of functional neuron-like cells from hNSCs, providing potent therapeutics for treating neurodegenerative diseases and neuronal disorders.

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“…These discoveries led us to further attempt to delineate effects of surface nanotopography and wettability Olivares-Navarrete, Rodil, et al 2015).…”

Section: Surface Roughnessmentioning

confidence: 99%

“…To more clearly determine the specific contributions of topography and chemistry, we compared responses of human MSCs and MG63 cells to smooth and microtextured titanium and to the same surfaces coated with a nanofilm of graphitic carbon (Olivares-Navarrete, Rodil, et al 2015). Osteogenic differentiation and maturation were enhanced on rougher surfaces, regardless of the chemistry.…”

Section: Cell Morphology and Integrin Signalingmentioning

confidence: 99%

Implant Surface Design Regulates Mesenchymal Stem Cell Differentiation and Maturation

et al. 2016

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Changes in dental implant materials, structural design, and surface properties can all affect biological response. While bulk properties are important for mechanical stability of the implant, surface design ultimately contributes to osseointegration. This article reviews the surface parameters of dental implant materials that contribute to improved cell response and osseointegration. In particular, we focus on how surface design affects mesenchymal cell response and differentiation into the osteoblast lineage. Surface roughness has been largely studied at the microscale, but recent studies have highlighted the importance of hierarchical micron/submicron/nanosurface roughness, as well as surface roughness in combination with surface wettability. Integrins are transmembrane receptors that recognize changes in the surface and mediate downstream signaling pathways. Specifically, the noncanonical Wnt5a pathway has been implicated in osteoblastic differentiation of cells on titanium implant surfaces. However, much remains to be elucidated. Only recently have studies been conducted on the differences in biological response to implants based on sex, age, and clinical factors; these all point toward differences that advocate for patient-specific implant design. Finally, challenges in implant surface characterization must be addressed to optimize and compare data across studies. An understanding of both the science and the biology of the materials is crucial for developing novel dental implant materials and surface modifications for improved osseointegration.

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Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

Cited by 93 publications

References 68 publications

Enhancing the osteoblastic differentiation through nanoscale surface modifications

Enhancing the osteoblastic differentiation through nanoscale surface modifications

Graphene Oxide Hierarchical Patterns for the Derivation of Electrophysiologically Functional Neuron-like Cells from Human Neural Stem Cells

Implant Surface Design Regulates Mesenchymal Stem Cell Differentiation and Maturation

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