Role of horizontal gene transfer in the dissemination of antimicrobial resistance in food animal production

Vinayamohan, Poonam Gopika; Pellissery, Abraham Joseph; Venkitanarayanan, Kumar

doi:10.1016/j.cofs.2022.100882

Cited by 38 publications

(27 citation statements)

References 61 publications

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“…The evolution of AMR and the dynamics of ARGs spread across these sectors are critical for predicting emerging pathogens and controlling AMR dissemination. HGT is primarily responsible for rapid AMR spread and dissemination of ARGs between bacteria and across species (Sun et al, 2020;Vinayamohan et al, 2022). AMR can be established either vertically by point mutations or horizontally by acquiring MGEs such as plasmids and transposons (Vinayamohan et al, 2022).…”

Section: Horizontal Gene Transfermentioning

confidence: 99%

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Metagenomic-based surveillance systems for antibiotic resistance in non-clinical settings

et al. 2022

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The success of antibiotics as a therapeutic agent has led to their ineffectiveness. The continuous use and misuse in clinical and non-clinical areas have led to the emergence and spread of antibiotic-resistant bacteria and its genetic determinants. This is a multi-dimensional problem that has now become a global health crisis. Antibiotic resistance research has primarily focused on the clinical healthcare sectors while overlooking the non-clinical sectors. The increasing antibiotic usage in the environment – including animals, plants, soil, and water – are drivers of antibiotic resistance and function as a transmission route for antibiotic resistant pathogens and is a source for resistance genes. These natural compartments are interconnected with each other and humans, allowing the spread of antibiotic resistance via horizontal gene transfer between commensal and pathogenic bacteria. Identifying and understanding genetic exchange within and between natural compartments can provide insight into the transmission, dissemination, and emergence mechanisms. The development of high-throughput DNA sequencing technologies has made antibiotic resistance research more accessible and feasible. In particular, the combination of metagenomics and powerful bioinformatic tools and platforms have facilitated the identification of microbial communities and has allowed access to genomic data by bypassing the need for isolating and culturing microorganisms. This review aimed to reflect on the different sequencing techniques, metagenomic approaches, and bioinformatics tools and pipelines with their respective advantages and limitations for antibiotic resistance research. These approaches can provide insight into resistance mechanisms, the microbial population, emerging pathogens, resistance genes, and their dissemination. This information can influence policies, develop preventative measures and alleviate the burden caused by antibiotic resistance.

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Section: Horizontal Gene Transfermentioning

confidence: 99%

“…HGT is primarily responsible for rapid AMR spread and dissemination of ARGs between bacteria and across species (Sun et al, 2020;Vinayamohan et al, 2022). AMR can be established either vertically by point mutations or horizontally by acquiring MGEs such as plasmids and transposons (Vinayamohan et al, 2022).…”

Section: Horizontal Gene Transfermentioning

confidence: 99%

Metagenomic-based surveillance systems for antibiotic resistance in non-clinical settings

et al. 2022

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“…The transmission of AR E. coli by HGT depends mainly on the fact that resistant plasmids can be transmitted between different environments and hosts [ 27 , 82 , 83 ]. HGT mainly involves ARGs carried in MGEs, such as transposons, plasmids, and integrons [ 84 , 85 ]. The transfer of ARGs between E. coli of animal origin and other bacterial populations by HGT is governed mainly by three mechanism modes, including conjugation, transformation, and transduction [ 84 , 86 , 87 ].…”

Section: Prevalence Of Ar E Coli From Farm Animal-...mentioning

confidence: 99%

“…Transformation refers to the transfer of short free DNA from the environment, which does not need living donor cells, and incorporation into recipient cells. Transduction is the process of transfer of DNA via bacteriophages [ 84 , 85 ]. Tetracyclines with sub-minimum inhibitory concentration (MIC) can promote conjugate transfer of E. coli plasmid (PR4) and accelerate HGT effects [ 90 ].…”

Section: Prevalence Of Ar E Coli From Farm Animal-...mentioning

confidence: 99%

Mini-Review: Antibiotic-Resistant Escherichia coli from Farm Animal-Associated Sources

Kong

Tian

et al. 2022

Antibiotics

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Escherichia coli is one of the most frequent causes of gastro-intestinal and extra-intestinal diseases in animals and humans. Due to overuse and misuse of antibiotics, recent years have seen a rapidly increasing prevalence of antibiotic-resistant (AR) Escherichia coli globally; particularly, AR E. coli from farm animal-associated sources and its antibiotic resistance genes (ARGs) are becoming a global concern, with clinical negative effects on both human and animal health. The aim of this review was to explore the prevalence trends of AR E. coli from farm animals, waste treatment, and aquatic environments. The disinfection methods of AR E. coli and possible alternatives to antibiotics were also highlighted. The current review highlights that the prevalence of AR E. coli from food animals, products, and animal waste is increasing at an alarming rate, but is reduced at waste treatment plants. Ultraviolet (UV) treatment, surface plasma oxidation, and biochar are commonly used to effectively eliminate AR E. coli. Some probiotics, plant extracts, and antimicrobial peptides (AMPs) are arousing interest as promising alternatives to antibiotics to fight against AR E. coli. The current review suggests that AR E. coli from farm animal-associated sources is prevalent and poses a serious global threat to public health. This review provides an avenue for further research, development, and application of novel strategies to minimize antibiotic resistance in E. coli of farm animal origin.

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“…This indicates that treatment for the infection of such ARG-hosted bacteria is likely to become extremely difficult for humans. Horizontal gene transfer, which is triggered by mobile genetic elements (MGEs), including plasmids, integrons, and transposons, could trigger and enhance the dissemination of ARGs among various bacterial populations and various species [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Shifts of Antibiotic Resistomes in Soil Following Amendments of Antibiotics-Contained Dairy Manure

Kang

Liu

Chen

et al. 2022

IJERPH

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Dairy manure is a nutrition source for cropland soils and also simultaneously serves as a contamination source of antibiotic resistance genes (ARGs). In this study, five classes of antibiotics including aminoglycosides, beta-lactams, macrolides, sulfonamides, and tetracyclines, were spiked in dairy manure and incubated with soil for 60 days. The high throughput qPCR and 16S rRNA amplicon sequencing were used to detect temporal shifts of the soil antibiotic resistomes and bacterial community. Results indicated dairy manure application increased the ARG abundance by 0.5–3.7 times and subtype numbers by 2.7–3.7 times and changed the microbial community structure in soils. These effects were limited to the early incubation stage. Selection pressure was observed after the addition of sulfonamides. Bacterial communities played an important role in the shifts of ARG profiles and accounted for 44.9% of the resistome variation. The incubation period, but not the different antibiotic treatments, has a strong impact on the bacteria community. Firmicutes and Bacteroidetes were the dominant bacterial hosts for individual ARGs. This study advanced our understanding of the effect of dairy manure and antibiotics on the antibiotic resistome in soils and provided a reference for controlling ARG dissemination from dairy farms to the environment.

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Role of horizontal gene transfer in the dissemination of antimicrobial resistance in food animal production

Cited by 38 publications

References 61 publications

Metagenomic-based surveillance systems for antibiotic resistance in non-clinical settings

Metagenomic-based surveillance systems for antibiotic resistance in non-clinical settings

Mini-Review: Antibiotic-Resistant Escherichia coli from Farm Animal-Associated Sources

Shifts of Antibiotic Resistomes in Soil Following Amendments of Antibiotics-Contained Dairy Manure

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