Role of homocysteine, cystathionine and methylmalonic acid measurement for diagnosis of vitamin deficiency in high‐aged subjects

Herrmann, Wolfgang; Schorr, Heike; Bodis, Marion; Knapp, Jean-Pierre; Müller, Andreas J.; Stein, Günter; Geisel, Juergen

doi:10.1046/j.1365-2362.2000.00746.x

Cited by 78 publications

(59 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Table 3 of the present study indicate that some elderly respondents with raised MMA levels also have abnormal plasma urea and creatinine concentration, and therefore impaired kidney function. Many other studies have also detected a link between raised blood MMA levels and impaired kidney function (Stabler et al, 1986;Rasmussen et al, 1990a, b;Joosten et al, 1993;Lindenbaum et al, 1994;Koehler et al, 1996;Norman, 1998Norman, , 1999van Asselt et al, 1998;Herrmann et al, 2000;Hvas et al, 2000). According to Whitehead et al (1994), the 97.5th centile of plasma creatinine in adults aged 25 -55 y is approximately 122 mmol=l (males) and 100 mmol=l (females), and for plasma urea it is 7.6 mmol=l (males) and 6.8 mmol=l (females).…”

Section: Discussionmentioning

confidence: 97%

“…Following the early studies that demonstrated the basic relationship between vitamin B 12 and MMA in man (Gompertz et al, 1967;Chanarin et al, 1973;Norman et al, 1982;Carmel, 2000), and which established reliable assay techniques for MMA (Stabler et al, 1986), were a number of key studies showing a surprisingly high prevalence of raised MMA levels in the plasma of older people, where it appeared to provide a more reliable indicator of functional vitamin B 12 status than serum or plasma vitamin B 12 concentrations per se (Lindenbaum et al, 1988;Rasmussen et al, 1989;Moelby et al, 1990;Joosten et al, 1993;Allen & Casterline, 1994;Lindenbaum et al, 1994;Koehler et al, 1996;Baik & Russell, 1999;Bjorkegren & Svardsudd, 1999;Herrmann et al, 2000). Clearly, the ageing process in itself, and the increasing prevalence of age-related medical conditions that impair vitamin B 12 absorption, both increase the probability that people will become vitamin B 12 -deficient as they grow older.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship between methylmalonic acid, homocysteine, vitamin B12 intake and status and socio-economic indices, in a subset of participants in the British National Diet and Nutrition Survey of people aged 65 y and over

et al. 2003

View full text Add to dashboard Cite

Objective: Assessment of functional vitamin B 12 status in a subset of the respondents in the British National Diet and Nutrition Survey of people aged 65 y and over. Setting: National Diet and Nutrition Survey: a British nationwide cross-sectional sample of people aged 65 y and over, living either in the community or in institutions such as nursing homes, during one calendar year spanning 1994 -1995. Methods: Methylmalonic acid (MMA) concentrations were measured in plasma samples from 313 subjects (ca 14% of those originally enrolled in the survey). The results were compared with those for serum vitamin B 12 , vitamin B 12 intakes and other status and intake estimates and with socio-demographic indices. Results: Of the NDNS participants overall, 20% had serum vitamin B 12 concentrations < 150 pmol=l. In the subset studied here, 24% of free-living and 46% of institution-living participants had MMA >0.5 mmol=l. Geometric mean MMA increased with age, from 0.25 mmol=l in people aged 65 -74 y to 0.38 mmol=l in people aged 85 þ y. There was little evidence for any gender difference in MMA. It was inversely correlated with serum vitamin B 12 and with red blood cell folate; it was positively correlated directly with total homocysteine, but not significantly with serum folate or with vitamin B 12 intake. Among respondents with high MMA, a subgroup had normal serum vitamin B 12 but higher-than-average plasma urea and creatinine. Socio-demographic co-variates of MMA included receipt of State income benefits, social class of head of household, and educational attainment. These indices were not correlated with serum vitamin B 12 . Conclusions: The progressive increase in MMA with age is metabolic evidence for increasing risk of functional vitamin B 12 deficiency with increasing age in older people. There is evidence that renal function is linked to high MMA in some older people. Age and renal function are thus both important when establishing upper reference limits for MMA. The socio-demographic observations suggest a link between poverty and poor functional vitamin B 12 status in older British people.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Relationship between methylmalonic acid, homocysteine, vitamin B12 intake and status and socio-economic indices, in a subset of participants in the British National Diet and Nutrition Survey of people aged 65 y and over

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Our hypothesis was based on the assumption that the previously reported lack of association with prognosis might be due to the low sensitivity for total vitamin B 12 as a measure of vitamin B 12 status, making it a poor screening test for deficiency (Naurath et al, 1995;Herrmann et al, 2000Herrmann et al, , 2003Herrmann et al, , 2005. Over the years different serum markers, isolated and in combination, have been evaluated trying to obtain an optimal screening test for deficiency.…”

Section: Discussionmentioning

confidence: 99%

Holotranscobalamin and methylmalonic acid as prognostic markers following an acute myocardial infarction

Aarsetøy

Valente²,

Reine

et al. 2007

Eur J Clin Nutr

View full text Add to dashboard Cite

Objective: To evaluate whether low levels of holotranscobalamin (holoTC) or elevated levels of methylmalonic acid (MMA), both indicators of vitamin B 12 deficiency, might predispose to new cardiovascular events following an acute myocardial infarction (MI). Design: A prospective prognostic study. Setting: One hospital center in Stavanger, Norway. Subjects: A total of 300 patients admitted with an acute MI. Methods: Registration of new TnT positive coronary events (defined as TnT40.05 mg/l and a typical MI pattern) and/or cardiac death during a median follow-up time of 45 months. Results: We compared the recurrence of events in the lowest quartile of holoTC (Q1o73.9 pmol/l) to the event rate above the 25% percentile (Q2-4). For methylmalonic acid (MMA) the same comparison was carried out for the upper quartile (Q4X0.24 mmol/l) as compared with the event rate below the 75% percentile (Q1-3). After 18 and 45 months of follow-up, the odds ratio (OR) for Q1 vs Q2-4 for holoTC was 1.15 (95% confidence interval (CI) 0.91-1.46, P ¼ 0.25) and 1.05 (95% CI 0.86-1.29, P ¼ 0.64), respectively. For MMA the OR for Q4 vs Q1-3 was 0.95 (95% CI 0.76-1.19, P ¼ 0.67) after 18 months and 1.01 (95% CI 0.83-1.23, P ¼ 0.90) after 45 months. Conclusion: This study showed no increased risk of future cardiovascular events associated with low levels of holoTC or high levels of MMA following an acute MI.

show abstract

“…Serum levels of the functional markers of vitamin B12 status tHcy, MMA and Cys were measured using gas-chromatography-mass-spectrometry (GCMS) as described elsewhere (26). Serum concentrations of folate were measured with a commercial, competitive chemiluminescence immunoassay (Bayer Diagnostics, Fernwald, Germany) using an ACS Centaur automated analyzer (Bayer Diagnostics, Fernwald, Germany).…”

Section: Subjects and Study Designmentioning

confidence: 99%

Enhanced bone metabolism in vegetarians – the role of vitamin B12 deficiency

Herrmann

Obeid²,

Schorr³

et al. 2009

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Background: Vitamin B12 deficiency and bone fractures are common in vegetarians. However, a direct relationship between vitamin B12 status and bone metabolism in vegetarians has not been tested sufficiently. Methods: Our study included 96 vegetarians (23 German vegans, and 54 German and 19 Indian lacto-, lacto-ovo-vegetarians) and 89 omnivores (Germans and Asian-Indian immigrants in Oman). Blood concentrations of total vitamin B12, holotranscobalamin (holoTC), 25OH-vitamin D (25(OH)D), total homocysteine (tHcy), methylmalonic acid (MMA), and the bone turnover markers (BTMs) bone alkaline phosphatase (BAP), osteocalcin (OC), pro-collagen type I N-terminal peptide (PINP) and C-terminal telopeptides of collagen I (CTx) were measured. Results: Vegetarians from both population groups exhibited significantly higher concentrations of tHcy, MMA, folate, and BAP, but lower concentrations of holoTC and cobalamin compared with omnivores from the same population. Additionally, German vegetarians had higher circulating activities of BAP as well as higher CTx, OC, and PINP compared with their omnivorous controls. HoloTC and MMA were correlated with OC, CTx and BAP. Subjects with low vitamin B12 status (holoTC F35 pmol/L and MMA

show abstract

Role of homocysteine, cystathionine and methylmalonic acid measurement for diagnosis of vitamin deficiency in high‐aged subjects

Cited by 78 publications

References 42 publications

Relationship between methylmalonic acid, homocysteine, vitamin B12 intake and status and socio-economic indices, in a subset of participants in the British National Diet and Nutrition Survey of people aged 65 y and over

Relationship between methylmalonic acid, homocysteine, vitamin B12 intake and status and socio-economic indices, in a subset of participants in the British National Diet and Nutrition Survey of people aged 65 y and over

Holotranscobalamin and methylmalonic acid as prognostic markers following an acute myocardial infarction

Enhanced bone metabolism in vegetarians – the role of vitamin B12 deficiency

Contact Info

Product

Resources

About