Role of guard-cell ABA in determining maximal stomatal aperture and prompt vapor-pressure-deficit response

Yaaran, Adi; Negin, Boaz; Moshelion, Menachem

doi:10.1101/218719

Cited by 4 publications

(7 citation statements)

References 63 publications

(68 reference statements)

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“…These results suggest that the main role of basal ABA under SD conditions is to maintain the stomata closed during the night hours. This finding is consistent with previous data showing that endogenous ABA accumulates to a maximum during the dark period 19,36 , and with results showing that ABA-insensitive plants display more open stomata in the dark 37 . In addition, our results show that endogenous basal ABA is necessary for full stomatal closure during dusk, although other factor(s) are probably involved given that aba2 seedlings at ZT9 display more open stomata than Col-0 (~21 μm 2 compared to ~12 μm 2 ) (Fig.…”

Section: Resultssupporting

confidence: 93%

PIF transcriptional regulators are required for rhythmic stomatal movements

Rovira

Veciana

Locascio

et al. 2023

Preprint

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Stomata govern the gaseous exchange between the leaf and the external atmosphere, and their function is essential for photosynthesis and the global carbon and oxygen cycles. Rhythmic stomata movements in daily dark/light cycles prevent water loss at night and allow CO2 uptake during the day. How the actors involved are transcriptionally regulated and how this might contribute to rhythmicity is largely unknown. Here, we show that morning stomata opening depends on the previous night period. The transcription factors PHYTOCHROME- INTERACTING FACTORS (PIFs) accumulate at the end of the night and directly induce the guard cell-specific K+ channel KAT1. Remarkably, PIFs and KAT1 are required for blue light- induced stomata opening. Together, our data establish a molecular framework for daily rhythmic stomatal movements under well-water conditions, whereby PIFs are required for accumulation of KAT1 at night, which upon activation by blue light in the morning leads to the K+ intake driving stomata opening.

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Section: Resultssupporting

confidence: 93%

PIF transcriptional regulators are required for rhythmic stomatal movements

Rovira

Veciana

Locascio

et al. 2023

Preprint

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“…Defects in several ABA biosynthesis and ABA signaling mutants in Arabidopsis increase steady-state stomatal conductances and thus shift VPD responses to higher stomatal conductance values (5,37). Recent time-resolved stomatal conductance analyses have led to the model that the rapid stomatal conductance response to VPD elevation occurs in parallel to the ABA signaling pathway (5,34). A signaling pathway parallel to ABA signaling is the CO 2 signaling pathway (46).…”

Section: Resultsmentioning

confidence: 99%

“…Early studies on ABA-deficient (aba1-1) and ABAinsensitive (abi1-1 and abi2-1) mutants suggested that ABA and ABA signaling are not necessary for high VPD-induced stomatal closure (33). This is supported by the investigation of transgenic lines expressing a guard-cell-targeted dominant abi1-1 protein phosphatase, which showed a wild-type (WT)-like stomatal response to a rapid increase in VPD, including in time-resolved gas-exchange analyses (34). Overexpression of the Arabidopsis abi1-1 mutant allele in gray poplar, Populus x canescens (Aiton) Sm., exhibited a defect in steady-state stomatal conductance in response to a VPD increase (35).…”

Section: Significancementioning

confidence: 97%

“…Recent studies suggest that steady-state basal ABA signaling occurs in guard cells (46)(47)(48), and a model has been proposed that may explain many of the above findings, in which basal ABA signaling may contribute to the stomatal VPD response by amplifying the hydroactive stomatal closure pathway (20), similar to CO 2 -triggered stomatal closure (46,47). Furthermore, several studies suggest a key role for ABA in adjusting the steady-state stomatal conductance (34,35). In the present study, we pursued analyses of other guard-cell signal-transduction pathways and higher-order mutants given the limited knowledge of early VPD sensing and signaling mechanisms.…”

Section: Significancementioning

confidence: 99%

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Raf-like kinases and receptor-like (pseudo)kinase GHR1 are required for stomatal vapor pressure difference response

Hsu

Takahashi

Merilo

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Stomatal pores close rapidly in response to low-air-humidity-induced leaf-to-air vapor pressure difference (VPD) increases, thereby reducing excessive water loss. The hydroactive signal-transduction mechanisms mediating high VPD–induced stomatal closure remain largely unknown. The kinetics of stomatal high-VPD responses were investigated by using time-resolved gas-exchange analyses of higher-order mutants in guard-cell signal-transduction branches. We show that the slow-type anion channel SLAC1 plays a relatively more substantial role than the rapid-type anion channel ALMT12/QUAC1 in stomatal VPD signaling. VPD-induced stomatal closure is not affected in mpk12/mpk4GC double mutants that completely disrupt stomatal CO2 signaling, indicating that VPD signaling is independent of the early CO2 signal-transduction pathway. Calcium imaging shows that osmotic stress causes cytoplasmic Ca2+ transients in guard cells. Nevertheless, osca1-2/1.3/2.2/2.3/3.1 Ca2+-permeable channel quintuple, osca1.3/1.7-channel double, cngc5/6-channel double, cngc20-channel single, cngc19/20crispr-channel double, glr3.2/3.3-channel double, cpk-kinase quintuple, cbl1/4/5/8/9 quintuple, and cbl2/3rf double mutants showed wild-type-like stomatal VPD responses. A B3-family Raf-like mitogen-activated protein (MAP)-kinase kinase kinase, M3Kδ5/RAF6, activates the OST1/SnRK2.6 kinase in plant cells. Interestingly, B3 Raf-kinase m3kδ5 and m3kδ1/δ5/δ6/δ7 (raf3/6/5/4) quadruple mutants, but not a 14-gene raf-kinase mutant including osmotic stress-linked B4-family Raf-kinases, exhibited slowed high-VPD responses, suggesting that B3-family Raf-kinases play an important role in stomatal VPD signaling. Moreover, high VPD–induced stomatal closure was impaired in receptor-like pseudokinase GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 (GHR1) mutant alleles. Notably, the classical transient “wrong-way” VPD response was absent in ghr1 mutant alleles. These findings reveal genes and signaling mechanisms in the elusive high VPD–induced stomatal closing response pathway.

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“…Stomatal imprints were taken from adaxial and abaxial epidermises of glasshouse‐grown plants, as described in Yaaran et al . (2019). See Methods S1 for a detailed description.…”

Section: Methodsmentioning

confidence: 99%

Tree tobacco (Nicotiana glauca) cuticular wax composition is essential for leaf retention during drought, facilitating a speedy recovery following rewatering

et al. 2022

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Summary Despite decades of extensive study, the role of cuticular lipids in sustaining plant fitness is far from being understood. We utilized genome‐edited tree tobacco (Nicotiana glauca) to investigate the significance of different classes of epicuticular wax in abiotic stress such as cuticular water loss, drought, and light response. We generated mutants displaying a range of wax compositions. Four wax mutants and one cutin mutant were extensively investigated for alterations in their response to abiotic factors. Although the mutations led to elevated cuticular water loss, the wax mutants did not display elevated transpiration or reduced growth under nonstressed conditions. However, under drought, plants lacking alkanes were unable to reduce their transpiration, leading to leaf death, impaired recovery, and stem cracking. By contrast, plants deficient in fatty alcohols exhibited elevated drought tolerance, which was part of a larger trend of plant phenotypes not clustering by a glossy/glaucous appearance in the parameters examined in this study. We conclude that although alkanes have little effect on whole N. glauca transpiration and biomass gain under normal, nonstressed conditions, they are essential during drought responses, since they enable plants to seal their cuticle upon stomatal closure, thereby reducing leaf death and facilitating a speedy recovery.

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Role of guard-cell ABA in determining maximal stomatal aperture and prompt vapor-pressure-deficit response

Abstract: 21Abscisic acid (ABA) is known to be involved in stomatal closure. However, its role in

Cited by 4 publications

References 63 publications

PIF transcriptional regulators are required for rhythmic stomatal movements

PIF transcriptional regulators are required for rhythmic stomatal movements

Raf-like kinases and receptor-like (pseudo)kinase GHR1 are required for stomatal vapor pressure difference response

Tree tobacco (Nicotiana glauca) cuticular wax composition is essential for leaf retention during drought, facilitating a speedy recovery following rewatering

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