Role of collagen cross-linking on equine wound contraction and healing

Sardari, Kamran; Kazemi, Hossein; Emami, Mohammadreza; Movasaghi, A R; Goli, Amir Afkhami

doi:10.1007/s00580-008-0789-0

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…Wound healing of equine limb wounds is, similarly to wound healing in humans, mainly dependent on epithelialization with contraction playing a minor role. 7,8 This makes equine limb wounds more similar to wounds in humans than rodent wounds, as healing in rodents occurs mainly by contraction. 4,32 We created full thickness wounds, as these resemble the dermal losses seen in most human chronic wounds.…”

Section: Discussionmentioning

confidence: 99%

“…5,6 Contrary to the situation in loose-skinned animals such as rodents, contraction of wound edges play a small role (20–25%) in extremity/limb healing in humans and horses. 7,8 Due to the similarities in skin anatomy and relative importance of contraction and epithelialization in healing, horses seem to be valid model animals for wound healing in humans. 9,10 Horses are prone to impaired wound healing and chronic wound formation, in particular when wounds are located on the limbs.…”

Section: Introductionmentioning

confidence: 99%

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An Equine Wound Model to Study Effects of Bacterial Aggregates on Wound Healing

Jørgensen

Bay

Skovgaard

et al. 2019

Advances in Wound Care

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Objective: Relevant animal models to study effects of bacterial aggregates on wound healing are lacking. We aimed at establishing an equine wound model with bacterial aggregates to investigate the impact of bacterial inoculation on normal (thorax) and impaired (limb) wound healing. Approach: Wounds were created on three limbs and both thorax sides of six horses. Twelve out of 20 wounds per horse were inoculated with 10 4 Staphylococcus aureus and 10 5 Pseudomonas aeruginosa on day 4. Healing was monitored until day 27 by clinical assessment, including wound scoring, surface pH measurements, and digital photography for area determination. Biopsies were used for bacterial culture and for peptide nucleic acid fluorescence in situ hybridization to detect bacterial aggregates. Results: Inoculated limb wounds healed slower than noninoculated limb wounds from day 10 onward ( p < 0.0001). Inoculated and noninoculated thorax wounds healed equally well and faster than limb wounds. The odds ratio of detecting bacterial aggregates in inoculated limb wounds was 7.1 (2.4–21.0, p = 0.0086) compared with noninoculated limb wounds and 36.2 (3.8–348, p = 0.0018) compared with thorax wounds. Innovation: This equine wound model with bacterial aggregates might be superior to other animal wound models, as both normal and impaired healing can be studied simultaneously. In this model, many aspects of wound healing, including novel treatments, may be studied. Conclusions: The impaired healing observed in inoculated limb wounds may be related to the persistent bacterial aggregates. Both in capability of clearing inoculated bacteria from the wounds and in healing pattern, thorax wounds were superior to limb wounds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Equine Wound Model to Study Effects of Bacterial Aggregates on Wound Healing

Jørgensen

Bay

Skovgaard

et al. 2019

Advances in Wound Care

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“…The picture was taken after focusing onto the wound shape. The following measurements were recorded using the ImageJ software analysis as described before [ 49 ]: Percentage of wound size at day (x) compared to day (0) = wound size at day (x) mm 2 / wound size at day (0) mm 2 × 100 Percentage of wound contraction = 100 – the percentage of wound size at day (x) Percentage of epithelialization = size of epithelialization area at day (x) mm 2 / size of the wound at day (x) mm 2 × 100 …”

Section: Methodsmentioning

confidence: 99%

“…The picture was taken after focusing onto the wound shape. The following measurements were recorded using the ImageJ software analysis as described before [49]:…”

Section: Planimetry Of Woundsmentioning

confidence: 99%

Evaluation of fish skin as a biological dressing for metacarpal wounds in donkeys

et al. 2020

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Background The use of biological dressings has recently emerged in the management of burns and wounds. The aim of the present study was to evaluate the Nile tilapia skin as a biological dressing for full-thickness cutaneous metacarpal wounds in donkeys. The study was conducted on nine clinically healthy donkeys (n = 9). Here, fish skin dressings were obtained from fresh Nile tilapia (Oreochromis niloticus and sterilized by immersion in silver nanoparticles (AgNPs) solution for 5 min, with no change in collagen content. Bilateral, circular full-thickness excisional skin wounds (2 cm in diameter) were created on the dorsal aspect of the mid-metacarpals of each donkey. Wounds on the right metacarpals (treated wounds, n = 9) were dressed with sterile fish skins, while wounds on the left metacarpals (control wounds, n = 9) were dressed with sterile non-adherent dressing pads without any topical applications. Wound dressings were changed weekly. Wounds were evaluated microbiologically, grossly, and histologically on days 7, 14, and 21 post-wound inductions. Results Fish skin-dressed wounds showed a significant (P < 0.0001) reduction in microbial counts (Total viable bacterial count, Staphylococcal count, and Coliform count), a significant (P < 0.0001) decrease in the wound size, and a significant reduction (P < 0.0001) in the epithelial gap compared to the untreated wounds. No frequent dressing changes were needed. Conclusions Fish skin dressing accelerated the wound healing process and efficiently inhibited the local microbial activity and exuberant granulation tissue formation suggesting its reliable and promising application for metacarpal wounds of donkeys.

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“…In healing wounds, newly formed collagen fibers are not as cross-linked compared to the control, which is evidenced by an increased viscoelasticity (more viscous response), and therefore reduce the ability of the wounded tissue to bounce back after the application of a load. In theory, a decrease of viscoelasticity in wounds, that is, cross-linking, promotes wound closure by providing pathways to the transmission of compressive forces and facilitates the return to the “natural state” . Although it should be considered as a positive characteristic of healing, excessive cross-linking of fibers during early wound healing can promote premature wound stiffening and potentially delay the closure, which is a pathological deviation from a successful healing path .…”

Section: Discussionmentioning

confidence: 99%

Biomechanics of Wound Healing in an Equine Limb Model: Effect of Location and Treatment with a Peptide-Modified Collagen–Chitosan Hydrogel

Sparks

Sigaeva

Tarraf

et al. 2020

ACS Biomater. Sci. Eng.

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The equine distal limb wound healing model, characterized by delayed re-epithelialization and a fibroproliferative response to wounding similar to that observed in humans, is a valuable tool for the study of biomaterials poised for translation into both the veterinary and human medical markets. In the current study, we developed a novel method of biaxial biomechanical testing to assess the functional outcomes of healed wounds in a modified equine model and discovered significant functional and structural differences in both unwounded and injured skin at different locations on the distal limb that must be considered when using this model in future work. Namely, the medial skin was thicker and displayed earlier collagen engagement, medial wounds experienced a greater proportion of wound contraction during closure, and proximal wounds produced significantly more exuberant granulation tissue. Using this new knowledge of the equine model of aberrant wound healing, we then investigated the effect of a peptide-modified collagen–chitosan hydrogel on wound healing. Here, we found that a single treatment with the QHREDGS (glutamine–histidine–arginine–glutamic acid–aspartic acid–glycine–serine) peptide-modified hydrogel (Q-peptide hydrogel) resulted in a higher rate of wound closure and was able to modulate the biomechanical function toward a more compliant healed tissue without observable negative effects. Thus, we conclude that the use of a Q-peptide hydrogel provides a safe and effective means of improving the rate and quality of wound healing in a large animal model.

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Role of collagen cross-linking on equine wound contraction and healing

Cited by 7 publications

References 20 publications

An Equine Wound Model to Study Effects of Bacterial Aggregates on Wound Healing

An Equine Wound Model to Study Effects of Bacterial Aggregates on Wound Healing

Evaluation of fish skin as a biological dressing for metacarpal wounds in donkeys

Biomechanics of Wound Healing in an Equine Limb Model: Effect of Location and Treatment with a Peptide-Modified Collagen–Chitosan Hydrogel

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