Role of caspases and apoptosis-inducing factor (AIF) in cladribine-induced apoptosis of B cell chronic lymphocytic leukemia

Involvement of Sox-4 in the cytochrome c-dependent AIF-independent apoptotic pathway in HeLa cells induced by Δ12-prostaglandin J2

“…In murine glioma GL261 cells, CDK inhibitor, flavopiridol, accompanied the release of cytochrome c together with translocation of AIF (Newcomb et al, 2003). And in cells from patients of B cell chronic lymphocytic leukemia and human leukemic cell lines, apoptosis were induced by cladribine activated caspase cascade and translocated AIF to nuclei at the same time (Isabel et al, 2001;Perez-Galan et al, 2002). These reports lead us to exam ine the m itochondrial pathways of 12 -PGJ2-induced apoptosis in HeLa cells.…”

Farnesyltransferase inhibitor BMS-214662 induces apoptosis in B-cell chronic lymphocytic leukemia cells

“…Lymphocytes were isolated from blood samples by Ficoll-density centrifugation as described. 7 Flow cytometry analysis revealed that 490% purified cells were CD5 þ , CD19 þ , CD23 þ and CD38 À . B-CLL cells were cultured at 5 Â 10 6 cell/ml in RPMI 1640 medium supplemented with 10% fetal calf serum, 2 mM L-glutamine, antibiotics and, except when indicated, 20 ng/ml IL-4 (hereafter, complete medium).…”

“…Apoptosis was analyzed by flow cytometry by determining the changes in cell forward/side scatter and through the simultaneous determination of phosphatidylserine (PS) exposure and mitochondrial membrane potential (DC m ) loss with annexin V-PE (Caltag) and DiOC 6 (3) (Molecular Probes), respectively. 7 In brief, B-CLL cells (5 Â 10 5 ) were incubated with 5 nM DiOC 6 (3) in 200 ml of annexinV-binding buffer (10 mM Hepes/NaOH, pH 7.4, 140 mM NaCl, 2.5 mM CaCl 2 ) at 371C for 15 min. Then, 1 ml of annexin V-PE (0.1 mg/ml) was added and cells incubated for further 20 min at room temperature.…”

“…7 For the detection of the active, phosphorylated form of Akt, B-CLL cells (5 Â 10 6 cells/ ml) were incubated with either 10 ng/ml PMA for 1 h or 20 ng/ml IL-4 for 6 h in the presence or the absence of 0.25 mM BMS-214662. To revert the effect of IL-4, 0.1 mM wortmannin was added as a control.…”

“…[4][5][6] Resistance to apoptosis induction by these drugs may be one of the causes of chemoresistance. 7 Therefore, identification of new agents that induce apoptosis in B-CLL cells resistant to purine analogs would be useful to improve the therapy of this disease.…”