Role of bone marrow derived-mesenchymal stem cells against gastric ulceration: Histological, immunohistochemical and ultrastructural study

Abstract

The current study aimed to assess the antiulcerogenic impact of mesenchymal bone marrow stem cells (BMMSCs) against gastric ulcer induced by the use of piroxicam in rats and to compare this effect with the antiulcer drug “Pantoloc ®” proton pump inhibitors. The study included histological, histochemical, immunohistochemical and ultrastructural examination in stomach of rats in different study groups. In the ulcerated group, the glandular region of the stomach displayed clear mucosal lesions occurring as perforations along the stomach axis. In addition, stomach displayed degeneration of surface mucous cells accompanied by pyknosis, vacuolation among parietal cells in ishmus region, basal region with vacuolated chief cells and karyolitic nucleus of parietal cells. Moreover, Stomach sections of ulcer model rats showed intensive immunoreactivity to cytokeratin 20, Cox 2 and PCNA. Findings of the present study have shown that BMMSCs have an ameliorative effect against piroxicam-induced gastric ulcer in rats. Collectively, the proposed work has shown that BMMSCs have a curative capacity as an antiulcer due to their high antioxidant activity. Further studies are required in molecular levels to understand the mechanism of action during treatment.

Protective Effect of Pomegranate Peels Extracts Against Stomach Peptic-Ulcer Induced By Brexin In Albino Rats

“…Our work showed a signi cant decrease in the polysaccharides secretion for the ulcer-model rats compared with the other groups (Antodine, pomegranate, and control). This result is in agreement with Alazzouni et al (2020), who reported a noticeable decrease in the mucus polysaccharides distribution at the mucosal lining of rats due to the treatment with a single oral dose of Brexin as NSAIDs drug. Also, this nding agrees with the results by Mahmoud andAbd El-Ghffar (2019).…”

“…Our electron microscopic results for the ulcer-model group rats showed that Brexin caused several ultrastructural changes in the mucous cell organelles, including an irregularity in the nucleus membrane, mitochondria degeneration, and vacuolated zymogenic secretory granules. These results are in agreement with Alazzouni et al (2020), who demonstrated that a single oral dose of Brexin in rats caused degeneration in surface mucous cells with an irregular pyknotic nucleus, fragmented rER, and mitochondria. Also, our results are in agreement with Halter et al (2001), who demonstrated that treatment with aspirin in humans caused gastro mucosal damage, which resulted in a severe intramucosal petechial hemorrhage and erosions.…”

“…This result indicated proliferations in the gastric tissue following Brexin application (Polo et al 2012). A similar result has been reported by Alazzouni et al (2020), who reported an intensive immunoreactivity to PCNA in rat stomach tissue following Brexin application Pantolo as NSAIDs drug.…”

Therapeutic Versus Preventative Use of Ginkgo biloba Extract (EGb 761) against Indomethacin-Induced Gastric Ulcer in Mice

“…The expression of COX-2 was up-regulated in the indomethacin-treated mice, but down-regulated in the EGb 761-treated and pre-treated groups. This strong immunoreactivity to COX-2 in the ulcer model group was consistent with other studies [ 78 , 79 ]. EGb 761 therapy was found to suppress COX-2 expression in several studies, which were linked to its anti-inflammatory activity [ 80 , 81 , 82 ].…”

Therapeutic approach of adipose-derived mesenchymal stem cells in refractory peptic ulcer

“…In a study by Alazzouni et al, the anti-inflammatory effect of bone marrow mesenchymal stem cells (BM-MSCs) on piroxicam-induced gastric ulcers in rats was compared with anti-inflammatory drugs such as Pantoloc, which is a proton pump inhibitor. This study showed that BM-MSCs have a therapeutic capacity as anti-ulcers due to high antioxidant activity [ 98 ]. In another research, ADMSCs were used in aspirin-induced gastric ulcers.…”