Role of Anisotropy in Tissue Engineering

Mitchell, Geoffrey R.; Tojeira, Ana

doi:10.1016/j.proeng.2013.05.100

Cited by 46 publications

(36 citation statements)

References 24 publications

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“…[ 11 ] Evidence has increasingly suggested that such anisotropic fi ber organizations induce cell elongation, promote cell migration, and regulate cell differentiation via integrin-mediated outside-in signaling. [ 12 ] Building various anisotropic features into electrospun matrices [13][14][15][16] is highly desirable because of its potential to better mimic the unique mechanical and biologic functions of tissue ECM and consequently guide preferred tissue formation. However, current electric fi eld intervention tactics can only modulate the fi ber arrangement without feasibility of accommodating many other hierarchical and complex characteristics of native tissues (e.g., various patterned organization of cells).…”

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confidence: 99%

Patterned Electrospun Nanofiber Matrices Via Localized Dissolution: Potential for Guided Tissue Formation

Jia

Lamarre

et al. 2014

Advanced Materials

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With the assistance of an ink-jet printer, solvent (the "ink") can be controllably and reproducibly printed onto electrospun nanofiber meshes (the "paper") to generate various micropatterns and subsequently guide distinct cellular organization and phenotype expression. In combination with the nanofiber-assisted layer-by-layer cell assembly, the patterned electrospun meshes will define an instructive microenvironment for guided tissue formation.

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confidence: 99%

Patterned Electrospun Nanofiber Matrices Via Localized Dissolution: Potential for Guided Tissue Formation

Jia

Lamarre

et al. 2014

Advanced Materials

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“…The use of isotropic porous scaffolds often results in the formation of a necrotic core due to random cell infiltration and associated constraints on nutrient diffusion, whereas anisotropic porous scaffolds inherently promote guided cell infiltration without affecting nutrient diffusion and therefore yield fully functional viable tissue. 30…”

Section: Typical Morphological Featuresmentioning

confidence: 99%

Bioengineering a pre-vascularized pouch for subsequent islet transplantation using VEGF-loaded polylactide capsules

et al. 2020

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“…48 In addition, while the overall electrospun fibrous mats are isotropic, individual nanofibers could be inherently anisotropic in their form and structure, mimicking the anisotropy of the cardiac tissue. 49 In our previous study, although collagen hydrogels (2 or 3 mg/mL) and 10 μM aza improved GATA4 and cTnI expression by days 12 and 28, respectively, 23 no cell alignment was evident (Supporting Information Fig. S1).…”

Section: Discussionmentioning

confidence: 76%

“…MSCs synthesize ECM molecules such as collagens, elastin, laminin, heparin sulfate, and aggrecan, and those matrix fibrils could provide new adhesion sites for better cell distribution and alignment . In addition, while the overall electrospun fibrous mats are isotropic, individual nanofibers could be inherently anisotropic in their form and structure, mimicking the anisotropy of the cardiac tissue …”

Section: Discussionmentioning

confidence: 99%

Cardiomyogenic differentiation of human bone marrow‐derived mesenchymal stem cell spheroids within electrospun collagen nanofiber mats

Joshi

Brennan

Beachley

et al. 2018

J Biomedical Materials Res

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Collagen is the major structural protein in myocardium and contributes to tissue strength and integrity, cellular orientation, and cell–cell and cell‐matrix interactions. Significant post‐myocardial infarction related loss of cardiomyocytes and cardiac tissue, and their subsequent replacement with fibrous scar tissue, negatively impacts endogenous tissue repair and regeneration capabilities. To overcome such limitations, tissue engineers are working toward developing a 3D cardiac patch which not only mimics the structural, functional, and biological hierarchy of the native cardiac tissue, but also could deliver autologous stem cells and encourage their homing and differentiation. In this study, we examined the utility of electrospun, randomly‐oriented, type‐I collagen nanofiber (dia = 789 ± 162 nm) mats on the cardiomyogenic differentiation of human bone marrow‐derived mesenchymal stem cells (BM‐MSC) spheroids, in the presence or absence of 10 μM 5‐azacytidine (aza). Results showed that these scaffolds are biocompatible and enable time‐dependent evolution of early (GATA binding protein 4: GATA4), late (cardiac troponin I: cTnI), and mature (myosin heavy chain: MHC) cardiomyogenic markers, with a simultaneous reduction in CD90 (stemness) expression, independent of aza‐treatment. Aza‐exposure improved connexin‐4 expression and sustained sarcomeric α‐actin expression, but provided only transient improvement in cardiac troponin T (cTnT) expression. Cell orientation and alignment significantly improved in these nanofiber scaffolds over time and with aza‐exposure. Although further quantitative in vitro and in vivo studies are needed to establish the clinical applicability of such stem‐cell laden collagen nanofiber mats as cardiac patches for cardiac tissue regeneration, our results underscore the benefits of 3D milieu provided by electrospun collagen nanofiber mats, aza, and spheroids on the survival, cardiac differentiation and maturation of human BM‐MSCs. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3303–3312, 2018.

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Role of Anisotropy in Tissue Engineering

Cited by 46 publications

References 24 publications

Patterned Electrospun Nanofiber Matrices Via Localized Dissolution: Potential for Guided Tissue Formation

Patterned Electrospun Nanofiber Matrices Via Localized Dissolution: Potential for Guided Tissue Formation

Bioengineering a pre-vascularized pouch for subsequent islet transplantation using VEGF-loaded polylactide capsules

Cardiomyogenic differentiation of human bone marrow‐derived mesenchymal stem cell spheroids within electrospun collagen nanofiber mats

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