Role for two conserved intermembrane space proteins, Ups1p and Ups2p, in intra-mitochondrial phospholipid trafficking.

Tamura, Yasushi; Onguka, Ouma; Hobbs, Alyson E. Aiken; Jensen, Robert E.; Iijima, Miho; Claypool, Steven M.; Sesaki, Hiromi

doi:10.1074/jbc.a111.338665

Cited by 22 publications

(61 citation statements)

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“…We show that conversion of PS to PE occurs at physiological temperatures in a time-dependent manner. The PE formation is efficient, and its time course is consistent with a pulse-chase study with [ 14 C]serine in which conversion of PS to PE was examined in cells (34). Previously, mitochondrial PE production has been studied using permeabilized cells and purified mitochondria (51)(52)(53)(54)(55).…”

Section: Discussionsupporting

confidence: 57%

“…We have shown previously that Ups1p promotes the export of PE from the IM to the OM in pulse-chase studies with [ 14 C]serine in cells (34). We suggest that, in PE metabolism, Ups1p is required for both the maintenance of Psd1p levels and the export of PE from the IM to the OM when cells are grown under fermentable conditions.…”

Section: Discussionmentioning

confidence: 57%

“…Mdm31p is an IM protein required for mitochondrial morphology (44,45). A recent study has shown that Mdm31p has functions similar to Ups1p in CL metabolism (34). Yta10p and Yta12p are subunits of m-AAA ATPases Associated with diverse cellular Activities protease in the IM (46).…”

Section: Psd1p Is Not Required For the Transfer Of Ps From The Om To mentioning

confidence: 99%

“…Previous studies have identified intermembrane space (IMS) proteins Ups1p and Ups2p, which regulate the metabolism of CL and PE in mitochondria (10,(32)(33)(34). Ups1p and Ups2p are homologous to each other and evolutionarily conserved.…”

mentioning

confidence: 99%

“…In addition to CL metabolism, Ups1p and Ups2p are also important for the metabolism of PE. It has been suggested that Ups1p promotes the export of PE from the IM to the OM after its synthesis (34). On the other hand, Ups2p negatively regulates and suppresses this process, and loss of Ups2p decreases levels of PE in mitochondria due to accelerated export (34).…”

mentioning

confidence: 99%

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Phosphatidylethanolamine Biosynthesis in Mitochondria

Tamura¹,

Onguka

Itoh³

et al. 2012

Journal of Biological Chemistry

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Background: Phosphatidylserine is imported into mitochondria and decarboxylated during phosphatidylethanolamine biosynthesis. Results: Phosphatidylserine is transported from the mitochondrial outer membrane to the inner membrane independently of two intermembrane space proteins, Ups1p and Ups2p, and phosphatidylserine decarboxylase, Psd1p. Conclusion: Transport and decarboxylation of phosphatidylserine are mechanistically separable reactions. Significance: Phosphatidylserine trafficking is important for the phosphatidylethanolamine metabolism in mitochondria.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 57%

Section: Psd1p Is Not Required For the Transfer Of Ps From The Om To mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Phosphatidylethanolamine Biosynthesis in Mitochondria

Tamura¹,

Onguka

Itoh³

et al. 2012

Journal of Biological Chemistry

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Identification of multi‐copy suppressors for endoplasmic reticulum‐mitochondria tethering proteins in Saccharomyces cerevisiae

et al. 2016

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In yeast, the endoplasmic reticulum (ER)-mitochondria encounter structure (ERMES) tethers the ER to mitochondria, but its primary function remains unclear. To gain insight into ERMES functions, we screened multi-copy suppressors of the growth-defective phenotype of mmm1Δ cells, which lack a core component of ERMES, and identified MCP1, MGA2, SPT23, and YGR250C (termed RIE1). Spt23 and Mga2 are homologous transcription factors known to activate transcription of the OLE1 gene, which encodes the fatty acid Δ9 desaturase. We found that Ole1 partially relieves the growth defects of ERMES-lacking cells, thus uncovering a relationship between fatty acid metabolism and ERMES functions.

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Metabolic channeling of lipids via the contact zones between different organelles

Hanada

2024

BioEssays

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Various lipid transfer proteins (LTPs) mediate the inter‐organelle transport of lipids. By working at membrane contact zones between donor and acceptor organelles, LTPs achieve rapid and accurate inter‐organelle transfer of lipids. This article will describe the emerging paradigm that the action of LTPs at organelle contact zones generates metabolic channeling events in lipid metabolism, mainly referring to how ceramide synthesized in the endoplasmic reticulum is preferentially metabolized to sphingomyelin in the distal Golgi region, how cholesterol and phospholipids receive specific metabolic reactions in mitochondria, and how the hijacking of host LTPs by intracellular pathogens may generate new channeling‐like events. In addition, the article will discuss how the function of LTPs is regulated, exemplified by a few representative LTP systems, and will briefly touch on experiments that will be necessary to establish the paradigm that LTP‐mediated inter‐organelle transport of lipids is one of the mechanisms of compartmentalization‐based metabolic channeling events.

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Role for two conserved intermembrane space proteins, Ups1p and Ups2p, in intra-mitochondrial phospholipid trafficking.

Cited by 22 publications

References 0 publications

Phosphatidylethanolamine Biosynthesis in Mitochondria

Phosphatidylethanolamine Biosynthesis in Mitochondria

Identification of multi‐copy suppressors for endoplasmic reticulum‐mitochondria tethering proteins in Saccharomyces cerevisiae

Metabolic channeling of lipids via the contact zones between different organelles

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