RNAi-Mediated Silencing of ITPK Gene Reduces Phytic Acid Content, Alters Transcripts of Phytic Acid Biosynthetic Genes, and Modulates Mineral Distribution in Rice Seeds

Karmakar, Aritra; Bhattacharya, Sananda; Sengupta, Suman; Ali, Nusrat; Sarkar, Sailendra Nath; Datta, Karabi; Datta, Swapan K.

doi:10.1016/j.rsci.2020.05.007

Cited by 34 publications

(24 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ali et al reported that RNAi-mediated silencing of MIPS gene of phytic acid metabolism pathway increased zinc, calcium, and magnesium concentration in milled rice grain along with iron [32]. A similar result of increment in metal concentration including zinc was found when another gene of phytic acid metabolism, IPK1, was silenced by Ali et al [33] and ITPK (OsITP/6K-1) was silenced by Karmakar et al [31]. RNAi-mediated silencing of negative regulators of iron in rice (i.e., OsHRZ2) also increased zinc concentration along with iron in grain when compared to control rice [29].…”

Section: High Zinc Ricementioning

confidence: 80%

“…Although these mutant lines are effective, they compromised crop yield and overall performance. As an alternative strategy, transgenic crops were developed by manipulating the phytic acid biosynthetic pathway (Figure 4) by RNA interference (RNAi)-mediated silencing its key enzymes [31,64,65]. Many attempts have been made to reduce the phytic acid concentration in rice by generating mutant varieties exhibiting a low phytic acid (lpa) phenotype [62,63].…”

Section: Low Phytate Rice By Using Rnai Technologymentioning

confidence: 99%

“…Karmakar et al reported phytic acid downregulation of 46.2% by seed-specific RNAi-mediated gene silencing of an inositol triphosphate kinases (ITPK) homolog (OsITP/6K-1) in Khitish indica rice variety and found an 1.3-fold increment of iron accumulation in seed with 1.6-fold zinc and 3.2-fold bioavailability of Pi [31].…”

Section: Low Phytate Rice By Using Rnai Technologymentioning

confidence: 99%

See 2 more Smart Citations

Rice Biofortification: High Iron, Zinc, and Vitamin-A to Fight against “Hidden Hunger”

Majumder

Datta

2019

Agronomy

Self Cite

View full text Add to dashboard Cite

One out of three humans suffer from micronutrient deficiencies called “hidden hunger”. Underprivileged people, including preschool children and women, suffer most from deficiency diseases and other health-related issues. Rice (Oryza sativa), a staple food, is their source of nutrients, contributing up to 70% of daily calories for more than half of the world’s population. Solving “hidden hunger” through rice biofortification would be a sustainable approach for those people who mainly consume rice and have limited access to diversified food. White milled rice grains lose essential nutrients through polishing. Therefore, seed-specific higher accumulation of essential nutrients is a necessity. Through the method of biofortification (via genetic engineering/molecular breeding), significant increases in iron and zinc with other essential minerals and provitamin-A (β-carotene) was achieved in rice grain. Many indica and japonica rice cultivars have been biofortified worldwide, being popularly known as ‘high iron rice’, ‘low phytate rice’, ‘high zinc rice’, and ‘high carotenoid rice’ (golden rice) varieties. Market availability of such varieties could reduce “hidden hunger”, and a large population of the world could be cured from iron deficiency anemia (IDA), zinc deficiency, and vitamin-A deficiency (VAD). In this review, different approaches of rice biofortification with their outcomes have been elaborated and discussed. Future strategies of nutrition improvement using genome editing (CRISPR/Cas9) and the need of policy support have been highlighted.

show abstract

Section: High Zinc Ricementioning

confidence: 80%

Section: Low Phytate Rice By Using Rnai Technologymentioning

confidence: 99%

Section: Low Phytate Rice By Using Rnai Technologymentioning

confidence: 99%

See 1 more Smart Citation

Rice Biofortification: High Iron, Zinc, and Vitamin-A to Fight against “Hidden Hunger”

Majumder

Datta

2019

Agronomy

Self Cite

View full text Add to dashboard Cite

show abstract

“…Phytic acid is the principal storage form of phosphorus (P) in rice grain, which will chelate with Zn to form a complex (O'Dell et al 1972;Iwai et al 2012). The negative relationship between phytic acid and Zn had been observed in previous studies, in which the decreased phytic acid in lpa mutants would increase grain Zn content (Karmakar et al 2020;Liu et al 2004). However, Sakai et al (2015) concluded that the phytic acid content did not affect the Zn accumulation in rice seed.…”

Section: Discussionmentioning

confidence: 96%

Combination of High Zn Density and Low Phytic Acid for Improving Zn Bioavailability in Rice (Oryza stavia L.) Grain

Wang

Meng

et al. 2021

Rice

View full text Add to dashboard Cite

Background Zn deficiency is one of the leading public health problems in the world. Staple food crop, such as rice, cannot provide enough Zn to meet the daily dietary requirement because Zn in grain would chelate with phytic acid, which resulted in low Zn bioavailability. Breeding new rice varieties with high Zn bioavailability will be an effective, economic and sustainable strategy to alleviate human Zn deficiency. Results The high Zn density mutant LLZ was crossed with the low phytic acid mutant Os-lpa-XS110–1, and the contents of Zn and phytic acid in the brown rice were determined for the resulting progenies grown at different sites. Among the hybrid progenies, the double mutant always displayed significantly higher Zn content and lower phytic acid content in grain, leading to the lowest molar ratio of phytic acid to Zn under all environments. As assessed by in vitro digestion/Caco-2 cell model, the double mutant contained the relatively high content of bioavailable Zn in brown rice. Conclusions Our findings suggested pyramiding breeding by a combination of high Zn density and low phytic acid is a practical and useful approach to improve Zn bioavailability in rice grain.

show abstract

“…However, reducing PA in grains results in reduced germination rate and grain weight (Sakai et al, 2015), as well as impaired seedling development, ultimately hampering their agricultural applications. Low-PA rice lines were obtained through RNAi-or antisense-mRNA-mediated post-transcriptional silencing of genes in the PA biosynthetic pathway (Ali et al, 2013a(Ali et al, , 2013bKarmakar et al, 2020) (supplemental Table 1, nos. 24-26).…”

Section: Genetic Biofortification Approaches For Znmentioning

confidence: 99%

Zinc in plants: Integrating homeostasis and biofortification

et al. 2022

View full text Add to dashboard Cite

Zinc plays many essential roles in life. As a strong Lewis acid that lacks redox activity under environmental and cellular conditions, the Zn 2+ cation is central in determining protein structure and catalytic function of nearly 10% of most eukaryotic proteomes. While specific functions of zinc have been elucidated at a molecular level in a number of plant proteins, wider issues abound with respect to the acquisition and distribution of zinc by plants. An important challenge is to understand how plants balance between Zn supply in soil and their own nutritional requirement for zinc, particularly where edaphic factors lead to a lack of bioavailable zinc or, conversely, an excess of zinc that bears a major risk of phytotoxicity. Plants are the ultimate source of zinc in the human diet, and human Zn deficiency accounts for over 400 000 deaths annually. Here, we review the current understanding of zinc homeostasis in plants from the molecular and physiological perspectives. We provide an overview of approaches pursued so far in Zn biofortification of crops. Finally, we outline a ''push-pull'' model of zinc nutrition in plants as a simplifying concept. In summary, this review discusses avenues that can potentially deliver wider benefits for both plant and human Zn nutrition.

show abstract

RNAi-Mediated Silencing of ITPK Gene Reduces Phytic Acid Content, Alters Transcripts of Phytic Acid Biosynthetic Genes, and Modulates Mineral Distribution in Rice Seeds

Cited by 34 publications

References 41 publications

Rice Biofortification: High Iron, Zinc, and Vitamin-A to Fight against “Hidden Hunger”

Rice Biofortification: High Iron, Zinc, and Vitamin-A to Fight against “Hidden Hunger”

Combination of High Zn Density and Low Phytic Acid for Improving Zn Bioavailability in Rice (Oryza stavia L.) Grain

Zinc in plants: Integrating homeostasis and biofortification

Contact Info

Product

Resources

About