RNA-templated DNA origami structures

Endo, Masayuki; Yamamoto, Shigehiro; Tatsumi, Koichi; Emura, Tomoko; Hidaka, Kumi; Sugiyama, Hiroshi

doi:10.1039/c3cc38804b

Cited by 51 publications

(43 citation statements)

References 16 publications

(17 reference statements)

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“…Furthermore, recent findings suggest that nuclease activity and immune recognition in vivo is reduced when either siRNAs are modified with 2′‐OMe groups or when DNA nanostructures are enveloped with a PEGylated lipid bilayer, and here we demonstrated a structure design that provided good stability in cell culture conditions over at least 24 h. These results suggest combined strategies of structure design and modification can yield sufficient structural stability. With respect to other delivery vehicles, the DNA origami approach allows unprecedented control over nanoscale geometry and precise functionalization with nucleotides (i.e., CpG sequences or RNA, a variety of proteins, inorganic nanomaterials, small drug molecules, and combinations thereof, which could provide opportunities to optimize nanostructure design and functionalization for cellular uptake, localization, tunable drug release and multifunctional drug delivery. It also offers the advantage of easy loading, which is not typical of many other nanoparticle delivery systems.…”

Section: Resultsmentioning

confidence: 99%

Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model

Halley

Lucas

McWilliams

et al. 2015

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Many cancers show primary or acquired drug resistance due to the overexpression of efflux pumps. A novel mechanism to circumvent this is to integrate drugs, such as anthracycline antibiotics, with nanoparticle delivery vehicles that can bypass intrinsic tumor drug-resistance mechanisms. DNA nanoparticles serve as an efficient binding platform for intercalating drugs (e.g. anthracyclines doxorubicin and daunorubicin, which are widely used to treat acute leukemias) and enable precise structure design and chemical modifications, for example for incorporating targeting capabilities. Here, we utilize DNA nanostructures to circumvent daunorubicin drug resistance at clinically relevant doses in a leukemia cell line model. We report the fabrication of a rod-like DNA origami drug carrier that can be controllably loaded with daunorubicin. We further directly verify that nanostructure-mediated daunorubicin delivery leads to increased drug entry and retention in cells relative to free daunorubicin at equal concentrations, which yields significantly enhanced drug efficacy. Our results indicate that DNA origami nanostructures can circumvent efflux pump-mediated drug resistance in leukemia cells at clinically relevant drug concentrations and provide a robust DNA nanostructure design that could be implemented in a wide range of cellular applications due to its remarkably fast self-assembly (~5 minutes) and excellent stability in cell culture conditions.

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Section: Resultsmentioning

confidence: 99%

Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model

Halley

Lucas

McWilliams

et al. 2015

Small

205

163

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show abstract

“…1b). We hypothesize that such a T-junction architecture will be equally compatible with RNA (A-form duplex) and DNA (B-form duplex) [23][24][25] . Here T a tail-L ab loop base pairing among the tiles will cause the tiles to tetramerize and form a square (Fig.…”

Section: Molecular Designmentioning

confidence: 99%

De novo design of an RNA tile that self-assembles into a homo-octameric nanoprism

Liu

Jiang

et al. 2015

Nat Commun

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Rational, de novo design of RNA nanostructures can potentially integrate a wide array of structural and functional diversities. Such nanostructures have great promises in biomedical applications. Despite impressive progress in this field, all RNA building blocks (or tiles) reported so far are not geometrically well defined. They are generally flexible and can only assemble into a mixture of complexes with different sizes. To achieve defined structures, multiple tiles with different sequences are needed. In this study, we design an RNA tile that can homo-oligomerize into a uniform RNA nanostructure. The designed RNA nanostructure is characterized by gel electrophoresis, atomic force microscopy and cryogenic electron microscopy imaging. We believe that development along this line would help RNA nanotechnology to reach the structural control that is currently associated with DNA nanotechnology.

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“…In addition to the classical DNA‐templated scaffold, RNA transcripts can also be employed as RNA scaffolds to build RNA–DNA hybrid origami. The group of Sugiyama successfully fabricated seven‐helix bundled rectangular structures and six‐helix bundled tubular structures by using RNA transcripts as scaffolds and DNA sequences as staple strands . The physical properties of the assembled RNA–DNA hybrid origami can be modified by the functionalized RNA scaffold.…”

Section: Evolution Of Dna Origami Nanotechnologymentioning

confidence: 99%

“…The group of Sugiyama successfully fabricateds even-helixb undled rectangular structures and sixhelix bundledt ubular structures by using RNA transcripts as scaffoldsa nd DNA sequences as staple strands. [20] The physical properties of the assembled RNA-DNA hybrid origami can be modifiedb yt he functionalized RNA scaffold. Moreover,v arious functions based on the structure of RNA, such as RNA catalysis and RNA interference (RNAi), can be introduced into the RNA-DNA hybrid origami.M eanwhile, Mao and co-workersa lso constructed ribbon,rectangle, and triangle RNA-DNA hybrid origami structures, depending on the length of the RNA scaffolds.…”

Section: Development Of Dna Origami Nanotechnologymentioning

confidence: 99%

Multifunctional DNA Origami Nanoplatforms for Drug Delivery

et al. 2019

Chemistry An Asian Journal

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DNA nanotechnology has been employed in the construction of self‐assembled nano‐biomaterials with uniform size and shape for various biological applications, such as bioimaging, diagnosis, or therapeutics. Herein, recent successful efforts to utilize multifunctional DNA origami nanoplatforms as drug‐delivery vehicles are reviewed. Diagnostic and therapeutic strategies based on gold nanorods, chemotherapeutic drugs, cytosine–phosphate–guanine, functional proteins, gene drugs, and their combinations for optoacoustic imaging, photothermal therapy, chemotherapy, immunological therapy, gene therapy, and coagulation‐based therapy are summarized. The challenges and opportunities for DNA‐based nanocarriers for biological applications are also discussed.

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RNA-templated DNA origami structures

Cited by 51 publications

References 16 publications

Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model

Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model

De novo design of an RNA tile that self-assembles into a homo-octameric nanoprism

Multifunctional DNA Origami Nanoplatforms for Drug Delivery

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