RNA Synthesis in Rat Seminal Vesicles: Stimulation by Testosterone

Wicks, Wesley D.; Kenney, Francis T.

doi:10.1126/science.144.3624.1346

Cited by 62 publications

(16 citation statements)

References 10 publications

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“…At this time no changes in the pool size of nucleotides were detected, but at longer times of exposure to the steroid changes do occur (Wicks & Kenney, 1964). This could mean that, in this tissue, the levels of RNA precursors are under androgen control.…”

Section: Transcriptional and Post-transcriptional Aspects Of The Mechmentioning

confidence: 80%

“…There are several lines of evidence demonstrating the stimulatory effect of androgens on RNA synthesis in various androgen-sensitive tissues, such as rat ventral prostate gland (Liao, 1968;Williams-Ashman & Reddi, 1971 (Wicks & Kenney, 1964;Dubovsky & Kochakian, 1973), mouse kidney (Avdalovic & Kochakian, 1969;Frieden & Ku, 1971;Dubovsky & Kochakian, 1973), flank organ (Giegel, Stolti, Weinstein & Frost, 1971), bone marrow (Minguell et al 1971;Sierralta et al 1974), submaxillary gland (Chrétien, 1972), muscle (Florini, 1970) and chick oviduct (Palmiter & Haines, 1973).…”

Section: Transcriptional and Post-transcriptional Aspects Of The Mechmentioning

confidence: 97%

See 1 more Smart Citation

Molecular Mechanism of Action of the Male Sex Hormones

Minguell¹,

Sierralta²

1975

Journal of Endocrinology

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Section: Transcriptional and Post-transcriptional Aspects Of The Mechmentioning

confidence: 80%

Section: Transcriptional and Post-transcriptional Aspects Of The Mechmentioning

confidence: 97%

Molecular Mechanism of Action of the Male Sex Hormones

Minguell¹,

Sierralta²

1975

Journal of Endocrinology

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“…Wicks & Kenny (1964), using radioactively labelled nucleotides, demonstrated a 50°, rise in uptake of radioactivity into seminal vesicle RNA only 70 min after Fig. 9.…”

Section: Discussionmentioning

confidence: 96%

Cell proliferation in the prostate complex of the castrate mouse

Alison

Wright

Morley

et al. 1976

Journal of Microscopy

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SUMMARY Cell proliferation during 100 h of continuous androgen challenge was studied in the seminal vesicle and coagulating gland of Balb/c mice castrated 3 days or 14 days prior to the first daily injection of 250 μg testosterone propionate. Continuous labelling with [3H] thymidine indicated that the seminal vesicle was almost totally responsive to androgen, as early as 3 days after castration, whereas the androgen sensitivity of the coagulating gland increased from 30% at 3 days after castration to 85% at 14 days after castration. In both tissues the magnitude of the proliferative reaction could be related to the extent of cell loss prior to stimulation. The duration of the pre‐replicative phase in the response of the seminal vesicle to androgen was 20–25 h both at 3 and 14 days after castration. In the coagulating gland the pre‐replicative phase was 40 h at 3 days after castration and 20 h at 14 days after castration. The maximum uptake of [7α‐3H] testosterone administered to mice 3 days after castration was significantly greater (P < 001) in the seminal vesicle compared to the coagulating gland. At 14 days the seminal vesicle and coagulating gland exhibited a similar capacity for uptake. The in vivo metabolism of [7α‐3H] testosterone was studied by thin layer chromatography 30 min and 120 min after administration. A high proportion of the radioactivity extracted from all the tissues was associated with highly polar steroids. At 3 days after castration, the seminal vesicle, 2 h after administration of radioactive testosterone, retained a much higher proportion of radioactivity associated with dihydrotestosterone than did the coagulating gland. The localization of steroid in mice 3 days after castration was studied by drymount autoradiography at intervals up to 2 h after the injection of [1,2,6,7(n)‐3H]‐testosterone. A heavier deposition of silver grains was observed over autoradiographs of the seminal vesicle. In the seminal vesicle the grains were primarily located over nuclear areas whereas in the coagulating gland the grains were diffusely distributed over both nuclear areas and over cytoplasmic areas.

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“…Using the in-vivo labelling technique, Wicks & Kenny (1964) observed an increase in the specific activity of RNA in the seminal vesicle of recently castrated rats within 30 min of testosterone administration. Mainwaring & Peterken (1971) reported that androgens induced a rapid and transient increase in incorporation of [3H]uridine into prostatic nuclei of castrated rats.…”

Section: Discussionmentioning

confidence: 96%

Androgenic Regulation of Rapidly Synthesized Rna in the Rat Ventral Prostate

Honma¹,

Noumura²

1975

Journal of Endocrinology

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The influence of castration on the incorporation of nucleotide precursors into RNA of isolated prostatic tissue has been investigated. Castration brought about an apparent increase of incorporation by increasing the specific activity of the uridine nucleotide pool and not by an enhancement in synthesis of rapidly labelled RNA. An actual decrease in this reaction was shown by dividing the radioactivity incorporated into RNA by the specific activity of the uridine nucleotide. Administration of testosterone brought about enhanced synthesis of RNA in the prostates of rats castrated for 3 days as early as 4 h after injection, reaching maximum effect at 8 h. No significant difference was found between the turnover rates of rapidly labelled RNA from castrated, androgen-treated castrated and intact rats. The increased prostatic permeability to nucleosides as an early action of androgens in this organ is discussed.

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RNA Synthesis in Rat Seminal Vesicles: Stimulation by Testosterone

Cited by 62 publications

References 10 publications

Molecular Mechanism of Action of the Male Sex Hormones

Molecular Mechanism of Action of the Male Sex Hormones

Cell proliferation in the prostate complex of the castrate mouse

Androgenic Regulation of Rapidly Synthesized Rna in the Rat Ventral Prostate

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