RNA Interference Links Oxidative Stress to the Inhibition of Heat Stress Adaptation

Spiró, Zoltán; Arslan, Mehmet Alper; Somogyvári, Milán; Nguyen, Minh Tú; Smolders, Arne; Dancsó, Balázs; Németh, Nóra; Elek, Zsuzsanna; Braeckman, Bart P.; Csermely, Péter; Sőti, Csaba

doi:10.1089/ars.2011.4161

Cited by 17 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, CDC37, HSP90, FKBP4, and AHSA1 have been shown to play an important role in RNAi-mediated gene silencing in mammals ( Pare et al 2013 ). Oxidative stress can induce RNAi silencing and subsequently reduced thermal tolerance in C. elegans ( Spiró et al 2012 ). Examining the role of RNAi in the stress response of fish is a potentially interesting line of future inquiry.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional Response to Acute Thermal Exposure in Juvenile Chinook Salmon Determined by RNAseq

Tomalty

Meek

Stephens

et al. 2015

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Thermal exposure is a serious and growing challenge facing fish species worldwide. Chinook salmon (Oncorhynchus tshawytscha) living in the southern portion of their native range are particularly likely to encounter warmer water due to a confluence of factors. River alterations have increased the likelihood that juveniles will be exposed to warm water temperatures during their freshwater life stage, which can negatively impact survival, growth, and development and pose a threat to dwindling salmon populations. To better understand how acute thermal exposure affects the biology of salmon, we performed a transcriptional analysis of gill tissue from Chinook salmon juveniles reared at 12° and exposed acutely to water temperatures ranging from ideal to potentially lethal (12° to 25°). Reverse-transcribed RNA libraries were sequenced on the Illumina HiSeq2000 platform and a de novo reference transcriptome was created. Differentially expressed transcripts were annotated using Blast2GO and relevant gene clusters were identified. In addition to a high degree of downregulation of a wide range of genes, we found upregulation of genes involved in protein folding/rescue, protein degradation, cell death, oxidative stress, metabolism, inflammation/immunity, transcription/translation, ion transport, cell cycle/growth, cell signaling, cellular trafficking, and structure/cytoskeleton. These results demonstrate the complex multi-modal cellular response to thermal stress in juvenile salmon.

show abstract

Section: Resultsmentioning

confidence: 99%

Transcriptional Response to Acute Thermal Exposure in Juvenile Chinook Salmon Determined by RNAseq

Tomalty

Meek

Stephens

et al. 2015

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

show abstract

“…Recent emphases on miRNAs have led to their enormous potential role in cellular machinery and gene regulation of the cells, and their involvement in almost every biological process [2,3,35]. Mir-451 is a unique microRNA which undergoes Ago2-dependent cytoplasmic biogenesis pathway, independent from Dicer activity [15,16].…”

Section: Discussionmentioning

confidence: 99%

p47phox and reactive oxygen species production modulate expression of microRNA-451 in macrophages

Ranjan

Lee

Karpurapu

et al. 2014

Free Radical Research

View full text Add to dashboard Cite

The production of microRNAs (miRNA) is influenced by various stimuli, including environmental stresses. We hypothesized that reactive oxygen species (ROS)-associated stress could regulate macrophage miRNA synthesis. miRNAs undergo unique steps of maturation processing through either one of two pathways of cytoplasmic processing. Unlike the canonical pathway, the regulation of alternative cytoplasmic processing of miRNA has not been fully elucidated yet. We cultured bone marrow derived macrophages (BMDM) from wild type (WT) and p47phox−/− mice and profiled miRNA expression using microarrays. We analyzed 375 miRNAs including four endogenous controls to normalize the data. At resting state, p47phox−/− BMDM has the markedly reduced expression of miR-451 compared to WT BMDM, without other significant differences. Unlike majority of miRNAs, miR-451 goes through the unique alternative processing pathway, in which Ago2 plays a key role. In spite of significant reduction of mature miR-451, however, its precursor form, pre-mir-451, was similar in both BMDMs, suggesting that the processing of pre-mir-451 is impaired in p47phox−/− BMDM. Moreover, p47phox−/− BMDM expressed significantly reduced level of Ago2. In contrast, Ago2 mRNA levels were similar in WT and p47phox−/− BMDM, suggesting a post-transcriptional defect of Ago2 production in p47phox−/− macrophages, which resulted in impaired processing of pre-miR-451. In order to examine the functional significance of miR-451 in macrophages, we cultured BMDMs from miR-451 knock-out mice. Of interest, miR-451-deficient BMDM exhibited reduced ROS generation upon zymosan stimulation, compared to WT BMDM. Our studies suggest functional crosstalk between ROS and miR-451 in the regulation of macrophage oxidant stress.

show abstract

“…Furthermore, induction of HSP70s is attenuated with aging . If that is not enough, heat shock protein induction in response to oxidative stress is complicated by the recent finding that, ironically, some ROS can impair HSP72 induction through RNA interference with microRNAs …”

Section: Targeted Hsp72 Delivery Versus Gene Inductionmentioning

confidence: 99%

“…47,48 If that is not enough, heat shock protein induction in response to oxidative stress is complicated by the recent finding that, ironically, some ROS can impair HSP72 induction 49 through RNA interference with microRNAs. 50 Bimoclomol was considered a promising inducer of HSP72 synthesis and was evaluated in the clinic for diabetic neuropathy. It failed to show significant advantages over a placebo in phase II clinical trials.…”

Section: Targeted Hsp72 Delivery Versus Gene Inductionmentioning

confidence: 99%

Targeted heat shock protein 72 for pulmonary cytoprotection

Parseghian

Hobson

Richieri

2016

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

Heat shock protein 72 (HSP72) is perhaps the most important member of the HSP70 family of proteins, given that it is induced in a wide variety of tissues and cells to combat stress, particularly oxidative stress. Here we review independent observations of the critical role this protein plays as a pulmonary cytoprotectant and discuss the merits of developing HSP72 as a therapeutic for rapid delivery to cells and tissues after a traumatic event. We also discuss the fusion of HSP72 to a cell-penetrating single-chain Fv (scFv) antibody fragment derived from mAb 3E10, referred to as Fv-HSP70. This fusion construct has been validated in vivo in a cerebral infarction model and is currently in testing as a clinical therapeutic to treat ischemic events and as a fieldable medical countermeasure to treat inhalation of toxicants caused by terrorist actions or industrial accidents.

show abstract

RNA Interference Links Oxidative Stress to the Inhibition of Heat Stress Adaptation

Abstract: These results uncover a novel post-transcriptional element in the regulation of heat stress adaptation under oxidative conditions that may have implications in disease susceptibility and aging.

Cited by 17 publications

References 49 publications

Transcriptional Response to Acute Thermal Exposure in Juvenile Chinook Salmon Determined by RNAseq

Transcriptional Response to Acute Thermal Exposure in Juvenile Chinook Salmon Determined by RNAseq

p47phox and reactive oxygen species production modulate expression of microRNA-451 in macrophages

Targeted heat shock protein 72 for pulmonary cytoprotection

Contact Info

Product

Resources

About