RNA Interference: A Novel Source of Resistance to Combat Plant Parasitic Nematodes

Banerjee, Sagar; Banerjee, Anamika; Gill, Sarvajeet Singh; Gupta, Om Prakash; Dahuja, Anil; Jain, Pradeep; Sirohi, Anil

doi:10.3389/fpls.2017.00834

Cited by 67 publications

(29 citation statements)

References 61 publications

(75 reference statements)

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“…This could be due to the fact that cuticle collagen genes belong to multigene family, therefore possibility of compensatory functions of similar genes could not be denied. However, the reduction in the nematode numbers and nematode multiplication factor is comparable to the previously reported studies on host delivered RNAi to combat PPNs (reviewed by Banerjee et al, 2017b ). Stacking of multiple genes for developing RNAi constructs for targeting more than one system of the nematode may be an efficient approach to achieve better degree of resistance in future studies.…”

Section: Discussionsupporting

confidence: 86%

Host Delivered RNAi of Two Cuticle Collagen Genes, Mi-col-1 and Lemmi-5 Hampers Structure and Fecundity in Meloidogyne incognita

et al. 2018

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Root-knot nematodes have emerged as devastating parasites causing substantial losses to agricultural economy worldwide. Tomato is the most favored host for major species of root-knot nematodes. Control strategies like use of nematicides have proved to be harmful to the environment. Other control methods like development of resistant cultivars and crop rotation have serious limitations. This study deals with the application of host generated RNA interference toward development of resistance against root-knot nematode Meloidogyne incognita in tomato. Two cuticle collagen genes viz. Mi-col-1 and Lemmi-5 involved in the synthesis and maintenance of the cuticle in M. incognita were targeted through host generated RNA interference. Expression of both Mi-col-1 and Lemmi-5 was found to be higher in adult females followed by egg masses and J2s. Tomato var. Pusa Ruby was transformed with the RNAi constructs of these genes to develop transgenic lines expressing the target dsRNAs. 30.80–35.00% reduction in the number of adult females, 50.06–65.73% reduction in the number of egg mass per plant and 76.47–82.59% reduction in the number of eggs per egg mass were observed for the T1 events expressing Mi-col-1 dsRNA. Similarly, 34.14–38.54% reduction in the number of adult females, 62.34–66.71% reduction in number of egg mass per plant and 67.13–79.76% reduction in the number of eggs per egg mass were observed for the T1 generation expressing Lemmi-5 dsRNA. The multiplication factor of M. incognita reduced significantly in both the cases and the structure of adult females isolated from transgenic plants were heavily distorted. This study demonstrates the role of the cuticle collagen genes Mi-col-1 and Lemmi-5 in the structure and development of M. incognita cuticle inside the host and reinforces the potential of host generated RNA interference for management of plant parasitic nematodes (PPNs).

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Section: Discussionsupporting

confidence: 86%

Host Delivered RNAi of Two Cuticle Collagen Genes, Mi-col-1 and Lemmi-5 Hampers Structure and Fecundity in Meloidogyne incognita

et al. 2018

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“…RNAi has been applied to a number of plant-parasitic nematode and insect-parasitic nematode species (Banerjee et al, 2017;Chaudhary et al, 2019;Lilley et al, 2012;Maule et al, 2011;Morris et al, 2017;Ratnappan et al, 2016). In the case of mammalian-parasitic nematodes, RNAi has been successful in some species but not others (Maule et al, 2011).…”

Section: Rnai In Parasitic Nematodesmentioning

confidence: 99%

Recent advances in functional genomics for parasitic nematodes of mammals

Castelletto

Gang

Hallem

2020

Journal of Experimental Biology

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Human-parasitic nematodes infect over a quarter of the world's population and are a major cause of morbidity in low-resource settings. Currently available treatments have not been sufficient to eliminate infections in endemic areas, and drug resistance is an increasing concern, making new treatment options a priority. The development of new treatments requires an improved understanding of the basic biology of these nematodes. Specifically, a better understanding of parasitic nematode development, reproduction and behavior may yield novel drug targets or new opportunities for intervention such as repellents or traps. Until recently, our ability to study parasitic nematode biology was limited because few tools were available for their genetic manipulation. This is now changing as a result of recent advances in the large-scale sequencing of nematode genomes and the development of new techniques for their genetic manipulation. Notably, skin-penetrating gastrointestinal nematodes in the genus Strongyloides are now amenable to transgenesis, RNAi and CRISPR/Cas9-mediated targeted mutagenesis, positioning the Strongyloides species as model parasitic nematode systems. A number of other mammalian-parasitic nematodes, including the giant roundworm Ascaris suum and the tissue-dwelling filarial nematode Brugia malayi, are also now amenable to transgenesis and/or RNAi in some contexts. Using these tools, recent studies of Strongyloides species have already provided insight into the molecular pathways that control the developmental decision to form infective larvae and that drive the host-seeking behaviors of infective larvae. Ultimately, a mechanistic understanding of these processes could lead to the development of new avenues for nematode control.

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“…HIGS strategy against parasitic nematodes employs an in planta approach in which susceptible host plants are engineered to express dsRNA of essential nematode genes and deliver resultant siRNAs to the feeding pests. These small regulatory RNAs achieve silencing of targeted endogenous gene transcripts in the nematodes and confer protection on host plants [20]. In this study, we targeted M. incognita PolA1 gene for HIGS considering that it plays a crucial role in protein synthesis and its effective silencing in eukaryotes (including nematodes) could lead to deleterious effects.…”

Section: Discussionmentioning

confidence: 99%

Mitigating Root Knot Nematode Propagation on Transgenic Tobacco via <i>in Planta</i> Hairpin RNA Expression of <i>Meloidogyne incognita</i>—Specific PolA1 Sequence

Chukwurah¹,

Poku²,

Yokoyama³

et al. 2019

AJPS

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Root knot nematodes are top priority nematode pests that significantly constrain agricultural productivity globally especially in developing countries. However, expressing double stranded RNA (dsRNA) of essential nematode genes in susceptible plants is known to confer protection against these pests via RNA silencing. This molecular-based strategy is called host induced gene silencing (HIGS) and the selection of appropriate target nematode gene is critical to its success. In this study, therefore, we focused on root knot nematode PolA1, an essential single copy nuclear gene encoding the largest subunit of RNA polymerase I enzyme and evaluated its effectiveness as a target in conferring nematode resistance on Agrobacterium-mediated transformed tobacco plants. Transgenic tobacco expressing Meloidogyne incognita-specific (MiS) dsRNA of PolA1 gene showed significant reduction in nematode fecundity and multiplication compared to wild type plants in both T 0 and T 1 generations. T 0 plants showed varying degrees of agronomic vigorover WT plants possibly due to varying levels of processed siRNA. However, production of MiS siRNAs in the transgenic plants coupled with significant reduction of PolA1 transcript expression in nematodes feeding on roots of transgenic plants provided evidence of HIGS. Taken together, our results show that PolA1 is a potentially effective target for HIGS-mediated reduction of root knot nematode damage on transgenic tobacco. Given the homology of our target sequence among Meloidogyne species, this protection could be broad range against other root knot nematodes aside M. incognita.

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RNA Interference: A Novel Source of Resistance to Combat Plant Parasitic Nematodes

Cited by 67 publications

References 61 publications

Host Delivered RNAi of Two Cuticle Collagen Genes, Mi-col-1 and Lemmi-5 Hampers Structure and Fecundity in Meloidogyne incognita

Host Delivered RNAi of Two Cuticle Collagen Genes, Mi-col-1 and Lemmi-5 Hampers Structure and Fecundity in Meloidogyne incognita

Recent advances in functional genomics for parasitic nematodes of mammals

Mitigating Root Knot Nematode Propagation on Transgenic Tobacco via <i>in Planta</i> Hairpin RNA Expression of <i>Meloidogyne incognita</i>—Specific PolA1 Sequence

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RNA Interference: A Novel Source of Resistance to Combat Plant Parasitic Nematodes

Cited by 67 publications

References 61 publications

Host Delivered RNAi of Two Cuticle Collagen Genes, Mi-col-1 and Lemmi-5 Hampers Structure and Fecundity in Meloidogyne incognita

Host Delivered RNAi of Two Cuticle Collagen Genes, Mi-col-1 and Lemmi-5 Hampers Structure and Fecundity in Meloidogyne incognita

Recent advances in functional genomics for parasitic nematodes of mammals

Mitigating Root Knot Nematode Propagation on Transgenic Tobacco via &lt;i&gt;in Planta&lt;/i&gt; Hairpin RNA Expression of &lt;i&gt;Meloidogyne incognita&lt;/i&gt;—Specific PolA1 Sequence

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Mitigating Root Knot Nematode Propagation on Transgenic Tobacco via <i>in Planta</i> Hairpin RNA Expression of <i>Meloidogyne incognita</i>—Specific PolA1 Sequence