2017
DOI: 10.21769/bioprotoc.2239
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Abstract: The RNA exosome complex plays a central role in RNA processing and regulated turnover. Present both in cytoplasm and nucleus, the exosome functions through associations with ribonucleases and various adapter proteins (reviewed in [Kilchert et al., 2016]). The RNA exosome-associated EXOSC10 protein is a distributive, 3’–5’ exoribonuclease. The following protocol describes an approach to monitor the ribonucleolytic activity of affinity-purified EXOSC10-containing RNA exosomes, originating from HEK-293 cells, as … Show more

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Cited by 2 publications
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“…The protocol designed to measure the ribonucleolytic activity of the fission yeast exosome complex was established based on a previously described procedure ( 43 ). Briefly, total cell extracts were prepared from frozen cells using freezer mill grinder and submitted to Csl4-TAP purification using magnetic Pan Mouse IgG beads (Dynabeads, Invitrogen).…”
Section: Methodsmentioning
confidence: 99%
“…The protocol designed to measure the ribonucleolytic activity of the fission yeast exosome complex was established based on a previously described procedure ( 43 ). Briefly, total cell extracts were prepared from frozen cells using freezer mill grinder and submitted to Csl4-TAP purification using magnetic Pan Mouse IgG beads (Dynabeads, Invitrogen).…”
Section: Methodsmentioning
confidence: 99%