Nondestructive testing (NDT) can play an important role in improving the quality and reliability of tropical hardwood as an engineering material. By means of these methods, the stiffness of the material can be determined and the information used to improve its structural performance. Although, it is a usual approach for qualifying the material used to manufacture engineered wood products made mainly from softwoods, it is not so common for tropical hardwoods. Additionally, the lack of information regarding properties of glulam beam made from these kinds of wood is evident. In this context, the paper aimed at evaluating the theoretical and experimental deflection of glulam beams made from the Brazilian hardwood lourovermelho (Sextonia rubra). Initially, the stiffness of each lamina was determined nondestructively using transverse vibration method (E dtv ), which has been demonstrated to be the most suitable method for this wood species. Then, ten 5-lamina glulam beams were assembled according to descending lamina E dtv values. The experimental evaluation was performed using a four-point bending schedule. In general, the theoretical values of deflection were 2% higher than the experimental ones. The transverse vibration showed to be a suitable method to both measure lamina stiffness and predict glulam beam deflection.