Rickettsial spotted fever in capoeirão Village, Itabira, Minas Gerais, Brazil

Vianna, Manoella Campostrini Barreto; Horta, Maurício Cláudio; Sangioni, Luís Antonio; Cortéz, Adriana; Soares, Rodrigo Martins; Mafra, Cláudio; Galvão, Márcio Antônio Moreira; Labruna, Marcelo Bahia; Gennari, Solange Maria

doi:10.1590/s0036-46652008000500009

Cited by 31 publications

(16 citation statements)

References 27 publications

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“…Nearly half of this capybara population was seroreactive to R. rickettsii, similarly to what has been found in other BSF-endemic areas, where 40-100% of horses or capybaras (main hosts of A. cajennense ) have been found to be seroreactive to R. rickettsii [20,45,46] . In a recent study [14], capybaras experimentally infected with R. rickettsii via infected A. cajennense ticks developed high antibody endpoint titers (8,192-32,768) from 30 to 146 days post infestation.…”

Section: Discussionsupporting

confidence: 79%

Rickettsial infection in Amblyomma cajennense ticks and capybaras (Hydrochoerus hydrochaeris) in a Brazilian spotted fever-endemic area

Krawczak

Nieri-Bastos

Nunes³

et al. 2014

Parasites & Vectors

108

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BackgroundBrazilian spotted fever (BSF), caused by the bacterium Rickettsia rickettsii, is the deadliest spotted fever of the world. In most of the BSF-endemic areas, capybaras (Hydrochoerus hydrochaeris) are the principal host for the tick Amblyomma cajennense, which is the main vector of BSF.MethodsIn 2012, a BSF case was confirmed in a child that was bitten by ticks in a residential park area inhabited by A. cajennense-infested capybaras in Itú municipality, southeastern Brazil. Host questing A. cajennense adult ticks were collected in the residential park and brought alive to the laboratory, where they were macerated and intraperitoneally inoculated into guinea pigs. A tick-inoculated guinea pig that presented high fever was euthanized and its internal organs were macerated and inoculated into additional guinea pigs (guinea pig passage). Tissue samples from guinea pig passages were also used to inoculate Vero cells through the shell vial technique. Infected cells were used for molecular characterization of the rickettsial isolate through PCR and DNA sequencing of fragments of three rickettsial genes (gltA, ompA, and ompB). Blood serum samples were collected from 172 capybaras that inhabited the residential park. Sera were tested through the immunofluorescence assay using R. rickettsii antigen.ResultsA tick-inoculated guinea pig presented high fever accompanied by scrotal reactions (edema and marked redness). These signs were reproduced by consecutive guinea pig passages. Rickettsia was successfully isolated in Vero cells that were inoculated with brain homogenate derived from a 3rd passage-febrile guinea pig. Molecular characterization of this rickettsial isolate (designated as strain ITU) yielded DNA sequences that were all 100% identical to corresponding sequences of R. rickettsii in Genbank. A total of 83 (48.3%) out of 172 capybaras were seroreactive to R. rickettsii, with endpoint titers ranging from 64 to 8192.ConclusionsA viable isolate of R. rickettsii was obtained from the tick A. cajennense, comprising the first viable R. rickettsi isolate from this tick species during the last 60 years. Nearly half of the capybara population of the residential park was seroreactive to R. rickettsii, corroborating the findings that the local A. cajennense population was infected by R. rickettsii.

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Section: Discussionsupporting

confidence: 79%

Rickettsial infection in Amblyomma cajennense ticks and capybaras (Hydrochoerus hydrochaeris) in a Brazilian spotted fever-endemic area

Krawczak

Nieri-Bastos

Nunes³

et al. 2014

Parasites & Vectors

108

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“…According to a research carried out in Southern Brasil, R. rickettsii positivity was found to be as 33.7% in dogs (22). However, in another study carried out in Minas Gerais, Brazil, it was 81.3% in dogs (26). In the study presented here, we found the serologic evidence of spotted fever group rickettsial infection in 54.0% of dogs examined.…”

Section: Discussioncontrasting

confidence: 54%

“…According to the research carried out in Minas Gerais, Brazil, a high prevalence was also found among dogs, 81.3% of them had antibody titers of 64 and 128 (26). This prevalence value is higher than others previously reported for dogs from BSF-endemic areas in Minas Gerais (13.68%) and São Paulo (25 to 66%) (8,11,12).…”

Section: Discussionmentioning

confidence: 72%

Prevalence of Rickettsia rickettsii infection in dogs from the urban and rural areas of western Turkey

SAVAŞAN¹

2013

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this study, rickettsial infections in dogs living in rural and urban areas were evaluated in the Western Turkey. Dog sera has been tested indirect immunofluorescence test (IFA) by containing Rickettsia rickettsii antigen. Dog blood sera samples were obtained from 250 dogs (50 dogs from urban area and 200 dogs from rural area) covering the two-year period between March 2009 -March 2011. For each sera, the antigen reactive with Rickettsia rickettsii constitute the end point titers were determined. A total of 250 dogs sera samples were tested. Dog sera samples were found 54.0% (135/250) seropositive and 43.2% (108/250) were found seronegative for the Rickettsia rickettsii. However, of the 2.8% (7/250 dog sera samples were found to be suspicious for the Rickettsia rickettsii at ≥ 1:64 -1:256 < titer. In this study, 7 (14.0%) dogs in urban areas were found to be seropositive and 128 (64.0%) dogs in rural areas were found to be seropositive in the ≥ 1:256 titer with the IFA test. In conclusion, titers of antibodies against Rickettsia rickettsii obtained from rural and urban dogs in Western Turkey appeared to be remarkable and more research needs to be done on this subject reveals.Key

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“…Meanwhile in horses, the prevalence of SFG rickettsioses in the present study (72.0 %) agreed with Lemos et al [11], Horta et al [36], Medeiros et al [31], Alves et al [37] and Riveros-Pinilla et al [14], who had detected rickettsiae serologically in horses using immunofluorescence. In addition, Vianna et al [38] and Pacheco et al [39] found that the prevalence rate of rickettsiae in horses ranged from 68 to 81 %. However, the detection of SFG rickettsiae in the present study indicated the possibility for these pathogens to be present in dogs and horses in Egypt, and the importance of these domestic animals as potential infection amplifiers which play a more dominant role in the persistence of rickettsiae in the nature than previously thought [2,7].…”

Section: Primer Amplifying Sequence Of Rickettsiae Wasmentioning

confidence: 99%

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

et al. 2019

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Rickettsial spotted fever in capoeirão Village, Itabira, Minas Gerais, Brazil

Cited by 31 publications

References 27 publications

Rickettsial infection in Amblyomma cajennense ticks and capybaras (Hydrochoerus hydrochaeris) in a Brazilian spotted fever-endemic area

Rickettsial infection in Amblyomma cajennense ticks and capybaras (Hydrochoerus hydrochaeris) in a Brazilian spotted fever-endemic area

Prevalence of Rickettsia rickettsii infection in dogs from the urban and rural areas of western Turkey

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

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