Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells

Abstract

Gingival recession (GR) potentially leads to the exposure of tooth root to the oral cavity microenvironment and increases susceptibility to dental caries, dentin hypersensitivity, and other dental diseases. Even though many etiological factors were reported, the specific mechanism of GR is yet to be elucidated. Given the species richness concerning marine biodiversity, it could be a treasure trove for drug discovery. In this study, we demonstrate the effects of a marine compound, (+)-rhodoptilometrin from crinoid, on gingival cell migration, wound healing, and oxidative phosphorylation (OXPHOS). Experimental results showed that (+)-rhodoptilometrin can significantly increase wound healing, migration, and proliferation of human gingival fibroblast cells, and it does not have effects on oral mucosa fibroblast cells. In addition, (+)-rhodoptilometrin increases the gene and protein expression levels of focal adhesion kinase (FAK), fibronectin, and type I collagen, changes the intracellular distribution of FAK and F-actin, and increases OXPHOS and the expression levels of complexes I~V in the mitochondria. Based on our results, we believe that (+)-rhodoptilometrin might increase FAK expression and promote mitochondrial function to affect cell migration and promote gingival regeneration. Therefore, (+)-rhodoptilometrin may be a promising therapeutic agent for GR.

NRF2 regulates the sensitivity of human NSCLC cells to cystine deprivation-induced ferroptosis via FOCAD-FAK signaling pathway

“…Even though our results indicates the function of FOCAD in the regulation of mitochondrial TCA cycle and ETC, the detailed mechanism is still unclear, and no evidences reveal the direct link between FOCAD and mitochondria. However, the position of focal adhesion kinase (FAK) in mitochondrial regulation has been demonstrated by several groups [ [34] , [35] , [36] , [37] ]. Therefore, the relationship between FOCAD and FAK was further investigated in our study because of the role of FOCAD in focal adhesion.…”

Sinularin, an Anti-Cancer Agent Causing Mitochondria-Modulated Apoptosis and Cytoskeleton Disruption in Human Hepatocellular Carcinoma

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Establish a teleost skin explant model for studying the fish-pathogen interaction

“…The thermal cycling pro le consisted of an initial denaturation at 95℃ for 2 min, followed by 40 cycles of denaturation at 95℃ for 3 sec, an appropriate annealing/extension temperature at 60℃, for 30 sec. The comparative Ct (ΔΔCt) method was used to evaluate the expression of candidate genes 60 . The housekeeping gene elongation factor 1-alpha (EF1-α) was used as the reference gene, and relative fold changes were calculated based on the cycle threshold (Ct) values generated by Real-Time PCR.…”