Rhodium nanocatalyst-based lateral flow immunoassay for sensitive detection of staphylococcal enterotoxin B

Rhodium‐Based Nanozymes: Recent Advances and Challenges

“…Xiaoli Cai et al synthesize Rh-mAb by hydrothermal method. [17] The DMF solution containing polymethyl meth-acrylate was mixed with aqueous Na 3 RhCl 6 and ascorbic acid to obtain a light brown transparent solution, which was held in a water bath to turn black. The samples were collected by centrifugation and washing to obtain Rh nanomaterials.…”

“…The main idea was to use a flow-through immunoassay technique, and the final quantitative detection was done by colorimetric detection. [17] Flow-through immuno-assay is an emerging rapid detection technique developed in recent years, and nanomaterials can further optimize the detection. [62] The detection method and mechanism (Figure 4d): The anti-SEB monoclonal antibody and Rh-based nanozymes are combined to form couples of Rh-anti-SEB mAbs immobilized on a conjugate pad, and then the sample SEB solution to be tested is added to the sample pad for detection.…”

“…The minimum detection concentration limit of SEB was 1.2 pg cm À 3 , 250-fold higher than that of commercial ELISA, with stable results and excellent detection (Figure 4a-4c). [17] spiked into whole milk sample before (red) and after amplification (blue) in a mixture of TMB and H 2 O 2 for 5 min using Rh labels. Black data dots represent the test line intensity of gold colloid-LFA.…”

“…made Rh‐LFA (Lateral flow immunoassay) to detect SEB. The main idea was to use a flow‐through immunoassay technique, and the final quantitative detection was done by colorimetric detection [17] . Flow‐through immunoassay is an emerging rapid detection technique developed in recent years, and nanomaterials can further optimize the detection [62] .…”

Ultrasensitive lateral flow immunoassay for staphylococcal enterotoxin B using nanosized fluorescent metal–organic frameworks

“…Lateral flow immunoassay (LFA), a widely used paper-based rapid diagnostic test, is of low-cost, simple and user-friendly, with high sensitivity and speed, thus attracting increasing interest and showing distinct advantages in biomarker detection. [1][2][3][4] Existing LFAs are mainly capable of target determinations through a specific antibody and a colorimetric reader. 5,6 Although many rapid and sensitive lateral flow assay platforms have been developed, the sensitivity of most LFAs is in the mM to μM range, which lacks the high sensitivity of laboratorybased testing methods, such as the enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR).…”