RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells

Sedzinski, Jakub; Hannezo, Édouard; Tu, Fan; Biro, Maté; Wallingford, John B.

doi:10.1242/dev.149286

Cited by 11 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with the force balance argument, cell-autonomous actin-based protrusion causes apical emergence (i.e. the opposite of extrusion), as is seen when multiciliated cells are added to an epithelium in Xenopus 70,71 . Thus, apical/basal contraction or expansion have distinct effects depending on whether they occur at the single-cell or population level.…”

Section: Localized Contraction and Expansion In Epithelial Morphogenesismentioning

confidence: 55%

Actin-based force generation and cell adhesion in tissue morphogenesis

Clarke

Martin

2021

Current Biology

View full text Add to dashboard Cite

Section: Localized Contraction and Expansion In Epithelial Morphogenesismentioning

confidence: 55%

Actin-based force generation and cell adhesion in tissue morphogenesis

Clarke

Martin

2021

Current Biology

View full text Add to dashboard Cite

“…Cytoskeletal forces are known to be integral cell autonomous features of the intercalation process. Actin-based forces, as well as small small GTPase activity, drive apical expansion of MCCs (Ioannou et al, 2013;Kim et al, 2012;Kulkarni et al, 2018;Sedzinski et al, 2016Sedzinski et al, , 2017. Additionally, we have previously shown that centriole number and the subsequent changes to microtubule (MT) accumulation can regulate the timing of apical insertion, such that cells with more centrioles or more MTs insert earlier than cells with less (Collins et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

Tubulin Acetylation Promotes Penetrative Capacity of Cells Undergoing Radial Intercalation

Collins

Kim

Ventrella

et al. 2021

Preprint

View full text Add to dashboard Cite

The post-translational modification of tubulin provides a wide diversity of differential functions to microtubule networks. Here we address the role of tubulin acetylation on the penetrative capacity of cells undergoing radial intercalation in the skin of Xenopus embryos. Radial intercalation is the process by which cells move apically and penetrate the epithelial barrier via inserting into the outer epithelium. As such there are two opposing forces that regulate the ability of cells to intercalate: the restrictive forces of the epithelial barrier versus the penetrative forces of the intercalating cell. By positively and negatively modulating tubulin acetylation specifically in the intercalating cells, the timing of intercalation can be altered such that cells with more acetylated microtubules penetrate the epithelium faster. Moreover, the Xenopus epithelium is a complex array of variable types of vertices and we find that intercalating cells preferentially penetrate at higher order "rosette" vertices as opposed to the more prevalent tricellular vertices. We observed differential timing in the ability of cells to penetrate different types of vertices, indicating lower order vertices represent more restrictive sites of insertion. Interestingly, we are able to shift the accessibility of early intercalating cells towards the more restrictive tricellular junctions by modulating the level of tubulin acetylation and the subsequent penetrative capacity of intercalating cells. Overall our data implicate tubulin acetylation in driving tissue penetration of intercalating cells.

show abstract

“…(a) basal bodies begin to migrate, dock, and become organized (uniformly spaced) at the apical membrane (Boutin & Kodjabachian, 2019; Spassky & Meunier, 2017). (b) F‐actin and microtubules undergo dramatic reorganization just below the apical surface (Sedzinski et al, 2017; Spassky & Meunier, 2017; Werner et al, 2011). While microtubules govern cilia polarity at the local scale, that is, the polarity between neighboring cilia within a multiciliated cell, F‐actin is required for coordination of cilia polarity at the global cell, that is, between ciliated cells (Werner et al, 2011).…”

Section: Patient Driven Gene Discovery and Characterization—case Studiesmentioning

confidence: 99%

Xenopus to the rescue: A model to validate and characterize candidate ciliopathy genes

Rao

Kulkarni

2021

Genesis

View full text Add to dashboard Cite

Summary Cilia are present on most vertebrate cells and play a central role in development, growth, and homeostasis. Thus, cilia dysfunction can manifest into an array of diseases, collectively termed ciliopathies, affecting millions of lives worldwide. Yet, our understanding of the gene regulatory networks that control cilia assembly and functions remain incomplete. With the advances in next‐generation sequencing technologies, we can now rapidly predict pathogenic variants from hundreds of ciliopathy patients. While the pace of candidate gene discovery is exciting, most of these genes have never been previously implicated in cilia assembly or function. This makes assigning the disease causality difficult. This review discusses how Xenopus, a genetically tractable and high‐throughput vertebrate model, has played a central role in identifying, validating, and characterizing candidate ciliopathy genes. The review is focused on multiciliated cells (MCCs) and diseases associated with MCC dysfunction. MCCs harbor multiple motile cilia on their apical surface to generate extracellular fluid flow inside the airway, the brain ventricles, and the oviduct. In Xenopus, these cells are external and present on the embryonic epidermal epithelia, facilitating candidate genes analysis in MCC development in vivo. The ability to introduce patient variants to study their effects on disease progression makes Xenopus a powerful model to improve our understanding of the underlying disease mechanisms and explain the patient phenotype.

show abstract

RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells

Cited by 11 publications

References 26 publications

Actin-based force generation and cell adhesion in tissue morphogenesis

Actin-based force generation and cell adhesion in tissue morphogenesis

Tubulin Acetylation Promotes Penetrative Capacity of Cells Undergoing Radial Intercalation

Xenopus to the rescue: A model to validate and characterize candidate ciliopathy genes

Contact Info

Product

Resources

About